The ichthyofauna of a poorly known area in the middle-southern Espinhaco mountain range, state of Minas Gerais, Brazil: diagnostics and identification keys Author dos Santos, Sergio Alexandre https://orcid.org/0000-0003-4340-4139 Museu Nacional, Universidade Federal do Rio de Janeiro, Departamento de Vertebrados, Quinta da Boa Vista s / n. CEP 20.940 - 040, Rio de Janeiro, RJ, Brazil sergio.pisces@gmail.com Author de Britto, Marcelo Ribeiro Museu Nacional, Universidade Federal do Rio de Janeiro, Departamento de Vertebrados, Quinta da Boa Vista s / n. CEP 20.940 - 040, Rio de Janeiro, RJ, Brazil text ZooKeys 2021 2021-08-03 1054 25 66 http://dx.doi.org/10.3897/zookeys.1054.67554 journal article http://dx.doi.org/10.3897/zookeys.1054.67554 1313-2970-1054-25 BAEC60898F874A56BAD08E2922E22F60 C0E2746E8DE65F77826FCC7FF2B54A4C Characidium sp. A Fig. 3C Distribution. Upper rio Santo Antonio , rio Doce basin. Diagnosis. Characidium sp. A differs from its congeners of this study by having very tapered snout; wide and conspicuous vertical bars in the lateral of body in both juveniles and adults specimens; longitudinal dark band occupying one or more scales; one-two dark, wide and conspicuous bands in half of caudal fin rays and another in the base of first and posterior caudal fin ray. Remarks. In this study, we refer Characidium sp. A such as a first putative new species from the rio Doce basin, due to differences in morphology and color pattern between this species and another similar ones from Southeastern Brazil river basins such as C. alipioi , C. grajahuense , C. lagosantense , C. lauroi , and C. timbuiense .