Systematic revision of the genus Peronia Fleming, 1822 (Gastropoda, Euthyneura, Pulmonata, Onchidiidae) Author Dayrat, Benoit Department of Biology, Pennsylvania State University, University Park, PA 16802, USA https://orcid.org/0000-0002-1514-4854 bdayrat@gmail.com Author Goulding, Tricia C. Department of Biology, Pennsylvania State University, University Park, PA 16802, USA Author Apte, Deepak Bombay Natural History Society, Hornbill House, Opp. Lion Gate, Shaheed Bhagat Singh Road, Mumbai 400 001, Maharashtra, India Author Aslam, Sadar Centre of Excellence in Marine Biology, University of Karachi, Karachi 75270, Pakistan https://orcid.org/0000-0002-4340-7885 Author Bourke, Adam College of Engineering, Information Technology and the Environment, Charles Darwin University, Ellengowan Dr, Casuarina, NT 0810, Australia Author Comendador, Joseph National Museum of the Philippines, Taft Ave, Ermita, Manila, 1000, Metro Manila, Philippines Author Khalil, Munawar Department of Marine Science, Universitas Malikussaleh, Reuleut Main Campus, Kecamatan Muara Batu, North Aceh, Aceh, 24355, Indonesia https://orcid.org/0000-0002-8264-5317 Author Ngo, Xuan Qu ảng Institute of Tropical Biology, Vietnam Academy of Science and Technology, 85 Tran Quoc Toan Street, District 3, Ho Chi Minh City, Vietnam & Graduate University of Science and Technology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Cau Giay, Hanoi, Vietnam https://orcid.org/0000-0003-2587-1999 Author Tan, Siong Kiat Lee Kong Chian Natural History Museum, 2 Conservatory Dr, National University of Singapore, 117377, Singapore Author Tan, Shau Hwai Centre for Marine and Coastal Studies, Universiti Sains Malaysia, 11800, Minden Penang, Malaysia & Marine Science Laboratory, School of Biological Sciences, Universiti Sains Malaysia, 11800, Minden Penang, Malaysia text ZooKeys 2020 972 1 224 http://dx.doi.org/10.3897/zookeys.972.52853 journal article http://dx.doi.org/10.3897/zookeys.972.52853 1313-2970-972-1 791674942E9242C38D1FD4DE7264D7B7 2751774FF66A5579AE66145BE5055C8E Peronia okinawensis Dayrat & Goulding sp. nov. Figs 17 , 18 , 19 , 20 Type material. Holotype . Japan • holotype, hereby designated, 27/25 mm [696-4 H]; Okinawa, Kunigami, Bay just SE of Cape Hedo; 26°51.803'N , 128°15.863'E ; 3 Jul 2004; G Paulay, J Jeller, M Malay & Y Hiratsuka leg.; reef flat; UF 352288. Additional material examined. Japan • 2 specimens 25/20 mm [696-3] and 20/17 mm [696-2]; same collection data as for the holotype; UF 352288. Distribution (Fig. 6 ). Endemic to Okinawa, Japan. Etymology. Peronia okinawensis is named after its type locality: okinawensis is a latinized adjective that agrees in gender (feminine) with the generic name ( ICZN 1999 : Article 31.2). Habitat. The only specimens known were found on a reef flat. Peronia okinawensis seems to be rare compared to P. verruculata (unit #1) but may be more abundant at some other sites in Okinawa. It would be interesting, in the future, to map in detail at what exact sites the three Peronia species that are sympatric in Okinawa ( P. okinawensis , P. peronii , and P. verruculata ) overlap or not, in Okinawa and possibly in the rest of the Ryukyu Islands. Color and morphology. No picture of live animals is available. The color of preserved specimens is beige mottled with darker areas dorsally and whitish ventrally. The dorsal notum of live animals is covered by dozens of papillae of various sizes. Some papillae bear black dorsal eyes at their tip. The number of papillae with dorsal eyes is variable (8-15). The largest specimens are 27 mm long. Digestive system (Figs 17A , 18 ). Examples of radular formulae are presented in Table 5 . The median cusp of the rachidian teeth is approximately 45 μm long. The hook of the lateral teeth is approximately 110 μm long. The intestinal loops are of type I, with a transitional loop oriented between 12 to 3 o'clock . Figure 17. Peronia okinawensis , Japan, Okinawa, holotype [696-4 H] (UF 352288) A digestive system, dorsal view, the arrow indicates the orientation of the transitional loop B posterior, hermaphroditic (female) reproductive system C anterior, male, copulatory apparatus. Scale bars: 3 mm ( A-C ). Abbreviations: ag accessory penial gland, dd deferent duct, ddg dorsal digestive gland, fgm female gland mass, hg hermaphroditic gland, i intestine, ms muscular sac, ov oviduct, pdg posterior digestive gland, ps penial sheath, rm retractor muscle, rs receptaculum seminis, sp spermatheca, st stomach, v vestibule. Figure 18. Radula, Peronia okinawensis , Japan, Okinawa A, B holotype [696-4 H] (UF 352288) C-F [696-2] (UF 352288) A left half rows of teeth B rachidian and innermost lateral teeth C rachidian and innermost lateral teeth D rachidian and lateral teeth E lateral teeth F outermost lateral teeth. Scale bars: 200 μm ( A ), 80 μm ( B ), 20 μm ( C, F ), 60 μm ( D ), 100 μm ( E ). Reproductive system (Figs 17B, C , 19 , 20 ). In the anterior (male) parts, the muscular sac of the accessory penial gland is less than 15 mm long. The hollow spine of the accessory penial gland is narrow, elongated, and straight or slightly curved, and its shape (including at its tip) varies between individuals. Its length ranges from 1.8 mm ([696-3] UF 352288) to 2.3 mm ([696-4 H] UF 352288). Its diameter at the conical base ranges from 240 to 300 μm . Its diameter at the tip ranges from 115 to 150 μm . The retractor muscle is shorter or longer than the penial sheath and inserts near the heart. Inside the penial sheath, the penis is a narrow, elongated, soft, hollow tube. Its distal end bears conical hooks which are less than 35 μm long. Diagnostic features (Table 4 ). Peronia okinawensis is characterized by a unique combination of anatomical traits: muscular sac shorter than 15 mm, intestinal loops of type I (with a transitional loop oriented between 12 and 3 o'clock ), retractor muscle inserting near the heart. Remarks. A new species name is needed because no existing name applies to the species described here. The specimen [696-2] was tentatively identified as Peronia cf. verruculata by Dayrat et al. (2011) . This identification should be disregarded because the specimen [696-2] belongs to the species described here (Figs 2 - 4 ). Peronia okinawensis is one of the four Peronia species in Japanese waters (Fig. 6 ). For a comparison of their geographic range, see remarks on P. setoensis . For their identification, see the identification key as well as Table 4 . It is possible that P. okinawensis is not strictly endemic to Okinawa. Figure 19. Penial hooks, Peronia okinawensis , Japan, Okinawa A, B holotype [696-4] (UF 352288) C, D [696-3] (UF 352288). Scale bars: 4 μm ( A, C, D ), 2 μm ( B ). Figure 20. Accessory penial gland spine, Peronia okinawensis , Japan, Okinawa A holotype [696-4] (UF 352288) B [696-3] (UF 352288) C [696-2] (UF 352288) D same as A ; E same as B ; F same as C . Scale bars: 400 μm ( A-C ), 40 μm ( D-F ).