Taxonomic review of the elampine cuckoo wasps from northeastern Brazil (Hymenoptera: Chrysididae), with the description of three new species Author Lucena, Daercio A. A. 0000-0002-3250-3775 Laboratório de Biologia Comparada e Abelhas (LBCA), Departamento de Biologia, Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto (FFCLRP), Universidade de São Paulo, Avenida Bandeirantes, 3900, 14040 - 901, Ribeirão Preto-SP, Brazil Universidade Federal do Pernambuco, Avenida Professor Moraes Rego S / N, 50670 - 901, Recife-PE, Brazil & daerciobio @ gmail. com; https: // orcid. org / 0000 - 0002 - 3250 - 3775 daerciobio@gmail.com Author Santos-Neto, Pedro E. 0000-0002-2353-529X pedrosantineto @ gmail. com; https: // orcid. org / 0000 - 0002 - 2353 - 529 X pedrosantineto@gmail.com Author Zanella, Fernando C. V. 0000-0002-4817-1373 Universidade Federal da Integração Latino-Americana (UNILA), Avenida Tancredo Neves, 6731, 85867 - 970, Foz do Iguaçu-PR, Brazil & fcvzanella @ gmail. com; https: // orcid. org / 0000 - 0002 - 4817 - 1373 fcvzanella@gmail.com Author Almeida, Eduardo A. B. 0000-0001-6017-6364 Laboratório de Biologia Comparada e Abelhas (LBCA), Departamento de Biologia, Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto (FFCLRP), Universidade de São Paulo, Avenida Bandeirantes, 3900, 14040 - 901, Ribeirão Preto-SP, Brazil Universidade Federal do Pernambuco, Avenida Professor Moraes Rego S / N, 50670 - 901, Recife-PE, Brazil & eduardoalmeida @ usp. br; https: // orcid. org / 0000 - 0001 - 6017 - 6364 eduardoalmeida@usp.br text Zootaxa 2022 2022-11-25 5213 3 201 235 journal article 194623 10.11646/zootaxa.5213.3.1 5729cbd6-fefb-4b94-a3f8-9a57c6c4f8d8 1175-5326 7359903 76F7CE54-A0D8-4004-B8F4-7A7D08166C33 Holopyga lunae Lucena sp. nov. ( Figs 13 , 14 ) Diagnosis. Holopyga lunae sp. nov. resembles H. luzulina Dahlbom. These species are distinguished from other Brazilian Holopyga based on the combination of the following features: moderate-sized body (about 7 mm ), tarsal claw with two subsidiary teeth, and shape of the head (higher than broad). The new species is distinguished from H. luzulina based on the following combination of characters: vertex densely punctate (vertex with transverse band of sparse tiny punctures in H. luzulina ); lateral margin of pronotum bordered with discrete carina (lateral margin of pronotum lacking carina in H. luzulina ); male S3 dark brown (male S3 metallic green in H. luzulina ). Description. Holotype , male ( Fig. 13 ). Body length: 6.9 mm . FIGURE 13. Holopyga lunae Lucena sp. nov. , holotype male [RPSP]. A. Habitus, lateral left view; B. Head and pronotum, dorsal view; C. Head, frontal view; D. Habitus, dorsal view; E. Head and mesosoma, lateral left view; F. Metasoma, dorsal view; G. Metasoma, lateral left view. Scale bars: 0.5 mm (B, C, G), 1 mm (A, D, E, F). Head. High 1.2× breadth; least distance between antennal rims 0.6× MOD; scape more or less cylindrical, gently curved medially, slightly wider submedially, 2.9× longer than its maximum width; F1 length 2.1× breadth, 1.4× longer than F2, F2 slightly longer than F3; F3–F10 subequal in size; F11 somewhat truncate apically; lower medial margin of clypeus straight; subantennal distance about 0.6× MOD; malar space shorter than 0.5× MOD; POL 2.1× OL; 0.8× OOL; lateral ocelli linked with transverse shallow sulcus; inner ocular margin slightly convergent submedially, LID 1.3× scape length; eye height 1.7× breadth. Mesosoma. Anterior declivity of pronotum with shallow medial pit, lateral margin bordered with discrete carina; notaulus and parapsidal signum faintly marked; R1 2 v very short, barely visible; inner margin of tarsal claw with two subsidiary teeth. Metasoma. T3 slightly depressed postero-medially, apical margin slightly truncate, with faintly marked medial notch, lateral margin slightly angulate. Coloration. Head mostly metallic green, with purplish blue tints around ocelli; base of mandible green with faint purplish blue reflection; golden highlights basally on inner margin of compound eye and on upper gena; scape, pedicel and base of stipe metallic green; apex of clypeus, half mandible and flagellomeres brown; mesosoma mainly metallic green, with purple tints anteriorly on pronotum, scutum, and posteriorly on metapectal-propodeal complex; bluish highlights on lateral pronotum, and metapleuron-propodeum; golden highlights on pronotal lobe, mesopleuron, and legs; tegula brown; wing membrane light fuscous with brown veins; coxa, femur, tibia and outer surface of basitarsomere green; distal tarsomeres and inner surface of basitarsomeres light brown; dorsum of metasoma mainly metallic green with light bluish highlights on terga; disc of anterior declivity of T1 purplish brown; T2 with anterior transverse purplish blue band; golden highlights laterally on terga, brighter on T3; T3 distal edge hyaline; laterotergites mostly metallic green, with dark margins; S1–S2 metallic green, S3 dark brown. Sculpture . Vertex and frons densely sculptured, becoming sparser on gena and lower on face; scapal basin densely cross-ridged, with small punctures marginally; clypeus punctulate, sparser on disc; scape densely punctulate; gena rugose-striate, with distinct longitudinal ridge; dorsum of mesosoma and mesopleuron densely foveate, larger and deeper on metanotum; lateral pronotal depression and metapleuron-propodeum with transverse striae; posterior mesopleuron slightly striate; outer surface of legs punctulate, except tarsomeres; dorsum of metasoma heavily sculptured, with double, dense, punctation pattern, with small punctures among larger foveae, becoming transversely ridged lateroposteriorly on T1–T2, and anterolaterally on T3; anterior declivity of T1 mostly impunctate, with only marginal punctures; laterotergites of T1–T2 sparsely punctulate, becoming denser on laterotergite of T3; S1–S2 sparsely punctulate, becoming much denser on S3. Vestiture . Head with short, sparse, pale setae on vertex, becoming longer and denser on frons, along inner ocular margin, gena, and occiput; scapal basin glabrous; clypeus with long, sparse, pale setae, becoming denser marginally; distal margin of galea and mandible with long, sparse, gold setae; scape with short, gold setae; flagellomeres with short, suberect, pale setae; eye glabrous (magnification above 100×); dorsum of mesosoma with short, erect, sparse, yellowish pale setae, becoming longer and denser on scutum and scutellum; propleuron with long, dense, pale setae; lateral pronotum and metapleuron-propodeum almost glabrous, except for some sparse marginal setae; wing membrane entirely setose; coxae and femora with long, dense, pale setae; protibia with some outstanding, long, pale setae; meso- and metatibiae with short, dense, even, pale setae; venter of tarsomeres with dense, even, spine-like setae; dorsum of metasoma with short, suberect, pale setae; S1 and S2 with long, sparse, pale setae; S3 with long, dense, pale setae. Female ( Fig. 14 ). Same as male, except: F1 length 2.4× breadth; F11 not truncate; LID 1.4× scape; eye with microtrichia; flagellomeres with short, decumbent, pale setae; lacking golden highlights on head, pronotum and metasoma; S3 metallic green; T3 not depressed postero-medially; T3 apical margin not truncate, lacking medial notch, and lateral margin not angulate. Material examined. Holotype ♂ . BRAZIL , Sergipe : Canindé de São Francisco , Sta. Maria , 20.vi.2005 , Debora Moura leg. / 23885 UFPE / L172 P874, Waltheria indica (RPSP) . Allotype ♀ : Canindé de São Francisco , Cana Brava , 30.viii.2002 / 10391 UFPE / L140 P755, Melochia tomentosa ( RPSP : 1♀ ); other paratypes : Canindé de São Francisco , Poço Verde , 24.v.2005 / 22577 UFPE / L132 P994, Cissus erosa ( RPSP : 1♂ ); Canindé de São Francisco , Cana Brava , 30.viii.2002 / 10292 UFPE / L139 P874, Waltheria indica ( RPSP : 1♀ ) . Alagoas : Piranhas , Poço da Ingazeira , Mata Ciliar , 29.vi.2005 , Debora Moura leg. / 23914 UFPE / L180 P1191, Hydrolea spinosa / 23998 UFPE / L180 P1063, Tephrosia purpurea ( CEDU-UNILA : 2♂ 1♀ ) . Distribution. BRAZIL ( Alagoas , Sergipe ) ( Fig. 17 ). FIGURE 14. Holopyga lunae Lucena sp. nov. , allotype female [RPSP]. A. Habitus, lateral left view; B. Head and pronotum, dorsal view; C. Head, frontal view; D. Habitus, dorsal view; E. Head and mesosoma, lateral left view; F. Metasoma, dorsal view; G. Metasoma, lateral left view. Scale bars: 0.5 mm (B, C, E, G), 1 mm (A, D, F). Host. Unknown. Floral records. Waltheria indica (Malvaceae) , Cissus erosa (Vitaceae) , Melochia tomentosa (Malvaceae) , Hydrolea spinosa (Hydroleaceae) , and Tephrosia purpurea (Fabaceae) . Remarks. The holotype is missing part of the right antenna, and right pro- and mesolegs. Male paratypes are lacking parts of their legs and flagellomeres. The allotype and the female paratype are lacking parts of their legs. Rosa & Xu (2015) designated the lectotype for H. luzulina Dahlbom and provided photographs of the male typespecimen hosted in the MRSN. Type locality of this species is an unknown locality in Brazil . We have examined one couple from Ribeirão Preto ( São Paulo state , southeastern Brazil —RPSP), and the male precisely matches the original description and diagnostic features observed in the images of the male lectotype of this species. According to Kimsey & Bohart (1991) , H. luzulina is widespread in the Neotropical region, reaching the south of the Nearctic region through Mexico and southwest USA , though, we did not find this species sympatric with H. lunae sp. nov. Etymology. The new species is named after Danielle Luna, first author´s wife.