New species and records of Diaporthe from Jiangxi Province, China
Author
Yang, Qin
The Key Laboratory for Silviculture and Conservation of the Ministry of Education, Beijing Forestry University, Beijing 100083, China & Forestry Biotechnology Hunan Key Laboratories, Central South University of Forestry and Technology, Changsha 410004, China & The Key Laboratory for Non-Wood Forest Cultivation and Conservation of the Ministry of Education, Central South University of Forestry and Technology, Changsha 410004, China
Author
Jiang, Ning
https://orcid.org/0000-0002-9656-8500
The Key Laboratory for Silviculture and Conservation of the Ministry of Education, Beijing Forestry University, Beijing 100083, China
Author
Tian, Cheng-Ming
https://orcid.org/0000-0002-3352-7664
The Key Laboratory for Silviculture and Conservation of the Ministry of Education, Beijing Forestry University, Beijing 100083, China
chengmt@bjfu.edu.cn
text
MycoKeys
2021
2021-01-14
77
41
64
http://dx.doi.org/10.3897/mycokeys.77.59999
journal article
http://dx.doi.org/10.3897/mycokeys.77.59999
1314-4049-77-41
FAE5387DCE535A42BEF6F4E81E9297AA
Diaporthe citri (H.S. Fawc.) F.A. Wolf, J. Agric. Res., Washington 33(7): 625, 1926.
Figure 4
Description.
Leaf spots subcircular to irregular, pale brown, with dark brown at margin. Pycnidia solitary, scattered on the leaf surface. Pycnidial conidiomata in culture, globose, erumpent, single or clustered in groups of 3-5 pycnidia, coated with hyphae, cream to yellowish translucent conidial droplets exuded from ostioles. Conidiophores reduced to conidiogenous cells. Conidiogenous cells hyaline, unbranched, septate, straight, slightly tapering towards the apex, 14.5-25
x
2-3
μm
. Alpha conidia hyaline, aseptate, rounded at one end, apex at the other end, usually with two large guttulate, (9.5-)10.5-12
x
3.5-4.5
μm
. Beta conidia not observed.
Figure 4.
Diaporthe citri
on
Citrus sinensis
(BJFC-S1658)
a, b
symptoms on leaves of host plant
c
culture on PDA (30d)
d
conidiomata
e
alpha conidia
f
conidiophores and alpha conidia. Scale bars: 10
μm
(
e, f
).
Culture characters.
Colony originally flat with white fluffy aerial mycelium, becoming greyish mycelium with age, with yellowish-cream conidial drops exuding from the ostioles.
Specimens examined.
China. Jiangxi Province: Ganzhou City, on leaves of
Citrus sinensis
,
24°59'44"N
,
115°31'01"E
, 13 May 2018,
Q. Yang
,
Y. Liu
&
Y.M. Liang
(BJFC-S1658; living culture: CFCC 53079 and CFCC 53080);
24°59'45"N
,
115°31'02"E
, 13 May 2018,
Q. Yang
,
Y. Liu
&
Y.M. Liang
(BJFC-S1659; living culture: CFCC 53081 and CFCC 53082).
Notes.
Diaporthe citri
is a widely distributed species in citrus-growing regions. In the present study, four isolates (CFCC 53079, CFCC 53080, CFCC 53081 and CFCC 53082) from symptomatic leaves of
Citrus sinensis
were congruent with
D. citri
, based on DNA sequence and morphological data (Fig.
1
). The clade was, therefore, confirmed to be
D. citri
.