Chalarus (Diptera: Pipunculidae) of Colombia, with description of two new species and an updated key to males of the Neotropical species Author Ramos-Pastrana, Yardany Universidad de la Amazonia, Grupo de Investigación en Entomología Universidad de la Amazonia - GIEUA-, Laboratorio de Entomología, - LEUA-, Florencia, Caquetá, Colombia: e-mail: ya. ramos @ udla. edu. co; ORCID: 0000 - 0002 - 3193 - 6659 & Instituto Nacional de Pesquisas da Amazônia, INPA, Manaus, Amazonas, Brazil; e-mails and ORCIDs: willkenia @ gmail. com, 0000 - 0001 - 7260 - 5760; jarafael @ inpa. gov. br, 0000 - 0002 - 0170 - 0514 Author Marques, Dayse Willkenia A. Instituto Nacional de Pesquisas da Amazônia, INPA, Manaus, Amazonas, Brazil; e-mails and ORCIDs: willkenia @ gmail. com, 0000 - 0001 - 7260 - 5760; jarafael @ inpa. gov. br, 0000 - 0002 - 0170 - 0514 Author Rafael, José Albertino Instituto Nacional de Pesquisas da Amazônia, INPA, Manaus, Amazonas, Brazil; e-mails and ORCIDs: willkenia @ gmail. com, 0000 - 0001 - 7260 - 5760; jarafael @ inpa. gov. br, 0000 - 0002 - 0170 - 0514 text Acta Entomologica Musei Nationalis Pragae 2023 2023-12-28 63 2 383 395 http://dx.doi.org/10.37520/aemnp.2023.024 journal article 10.37520/aemnp.2023.024 1804-6487 10621434 B94E1D89-5827-404D-821F-2DA8339FFB58 Chalarus connexus Rafael, 1988 ( Figs 33–43 , 55 ) Chalarus connexus Rafael, 1988: 6 , Figs 1–13, 20–21 . Chalarus connexus : RAFAEL (1990: 48) ; JERVIS (1992: 346) ; DE MEYER (1996:15) ; KEHLMAIER &ASSMANN (2010:12) ; RODRÍGUEZ & RAFAEL (2012: 17, 28, 43). Material examined (1 J). COLOMBIA , Caquetá , San Vicente del Caguán, / Vda.[Vereda] Alto Quebradón, Fca.[Finca] Rancho Veracruz , / Bosque piso, 02º17’55.8’’N / 74º44’29.3’’W , / 414 m [eters], 1–15. Feb[ II ].2017, / trampa Malaise en el piso del bosque, / Y.Ramos-Pastrana ( LEUA –00000049808) (photographed specimen). Diagnosis. Cell r 1 closed to the confluence of the veins R 1 and R 2+3. Both surstyli with one lobe apically, inward directed, inner margins curved and outer margins nearly straight; both surstyli without sinus at junction with epandrium; left surstylus with lower margin rounded; right surstylus with upper margin nearly straight; both surstyli with apex upwards directed, without sinus in union with epandrium in lateral view. Gonopods thickened, with apices acute; right gonopod slightly more acute than left one. Tip of distiphallus straight and acute, without phallic processes. Phallus trifid, all ejaculatory ducts situated little before apex; lateral ejaculatory ducts with inner margins forming straight line in dorsal view. Intraspecific variability. RAFAEL (1988) provided a complete description and illustration of the Brazilian holotype ; however, we found intraspecific variation, therefore, we provide a comparison of the Colombian and Brazilian specimens (between parenthesis, when comparable). Male . Head ( Figs 33–34 ). Occiput dark brown, brown pruinose. Antenna brown ( Fig. 35 ) ( versus antenna black in the holotype ). Thorax ( Figs 34–36 ). Postpronotal lobe light brown, light brown pruinose. Notopleuron concolorous with scutum. Mesopleuron brown, brown pruinose. Mediotergite brown pruinose. Wing ( Fig. 37 ). Length 2.2 mm ( versus 1.8 mm ), width 0.7 mm ( versus 0.6 mm ), membrane slightly infuscate, pterostigma occupying 2/3 of third costal section ( versus pterostigma occupying a little more than half of third costal section). Halter brown ( versus halter dark brown). Abdomen ( Figs 34–38 ). Tergite 1 light brown basally, dark brown posterolaterally; syntergosternite 8 dark brown, gray-brown pruinose, with long apical setae. Terminalia ( Figs 39–43 ). Epandrium and surstyli brown ( Fig. 39 ). Surstyli ( Figs 39–41 ) subsymmetrical, completely setose, with long setae apically; both surstyli with one lobe apically, directed inward, inner margin curved and outer margin nearly straight and lower margin slightly truncated ( Fig. 39 ); left surstylus with lower margin rounded, right surstylus with upper margin nearly straight, both surstyli with apex upwards directed, without sinus in union with epandrium in lateral view ( Figs 40–41 ). Gonopods subsymmetrical, thickened, with apices acute, right slightly more acute than left one ( Figs 40–41 ). Tip of distiphallus straight and acute, without phallic processes ( Fig. 42A ). Ejaculatory apodeme parasol-shaped ( Fig. 43 ). Phallus trifid, all ejaculatory ducts situated little before apex in dorsal and lateral view ( Figs 42A–B ). Habitat. The specimen was collected in preserved areas of tropical rainforest in the Colombian Amazon, Department of Caquetá . Geographical distribution. Brazil ( Amazonas ) ( RAFAEL 1990 ), Colombia ( Caquetá ) (new record) ( Fig. 55 ).