A species-level taxonomic review and host associations of Glyptapanteles (Hymenoptera, Braconidae, Microgastrinae) with an emphasis on 136 new reared species from Costa Rica and Ecuador Author Arias-Penna, Diana Carolina Author Whitfield, James B. Author Janzen, Daniel H. Author Winifred Hallwachs, Author Dyer, Lee A. Author Smith, M. Alex Author Hebert, Paul D. N. Author Fernandez-Triana, Jose L. text ZooKeys 2019 890 1 685 http://dx.doi.org/10.3897/zookeys.890.35786 journal article http://dx.doi.org/10.3897/zookeys.890.35786 1313-2970-890-1 FD8F695311F64DF2950F6A387340BCE5 2691DADB7BA352BEBA377C901FC0AC97 Glyptapanteles stephaniecluttsae Arias-Penna, sp. nov. Figs 204 , 205 Female. Body length 2.17 mm , antenna length 2.73 mm [distal flagellomere is missing], fore wing length 2.48 mm . Type material. Holotype : COSTA RICA • 1♀ ; 07-SRNP-2903, DHJPAR0020275; Area de Conservacion Guanacaste , Guanacaste , Sector San Cristobal , Sendero Vivero ; rain forest; 730 m ; 10.86739 , -85.38744 ; 22.vi.2007 ; Elda Araya leg.; caterpillar collected in fourth instar; dark brown hard cocoons adhered to the leaf substrate; ( CNC ) . Paratypes . • 5 (0 ♀ , 1♂ ) ( 4♀ , 0 ♂ ); 07-SRNP-2903, DHJPAR0020275; same data as for holotype; ( CNC ) . Diagnosis. Lateral grooves delimiting the median area on T2 distally losing definition, edges of median area on T2 polished and followed by a deep groove ( Figs 204H , 205D ), axillary trough of metanotum completely smooth ( Figs 204G , 205C ), precoxal groove shallow, but visible ( Figs 204J , 205E ), anteroventral contour of mesopleuron straight/angulate or nearly so ( Figs 204J , 205E ), and fore wing with r vein curved, outer side of junction of r and 2RS veins forming a distinct stub ( Fig. 204L ). Figure 204. Glyptapanteles stephaniecluttsae sp. nov. female 07-SRNP-2903 DHJPAR0020275 A Habitus B, D Head B Frontal view D Dorsal view C Head, pronotum, propleuron, lateral view E Genitalia: hypopygium, ovipositor, ovipositor sheaths, lateral view F Mesonotum, dorsal view G Scutellum, metanotum, propodeum, dorsal view H T1-3, dorsal view I, K Metasoma I Dorsal view K Lateral view J Mesosoma, lateral view L Fore and hind wings. Figure 205. Glyptapanteles stephaniecluttsae sp. nov. male 07-SRNP-2903 DHJPAR0020275 A Habitus B Mesonotum, dorsal view C Scutellum, metanotum, propodeum, dorsal view D T1-2, dorsal view E Mesosoma, lateral view F, G Metasoma F Lateral view G Dorsal view. Coloration ( Fig. 204 A-L ). General body coloration yellow except dark brown-black head; scape and pedicel yellow-brown; all antennal flagellomeres dorsally lighter (light brown) that ventrally (dark brown); tegulae yellow; mesoscutum brown; lunules and area between lunules, ATS groove, PFM dark brown-black; glossa, maxillary and labial palps pale yellow/ivory. Eyes silver and ocelli yellow. Fore and middle legs dark yellow except brown claws; hind legs dark yellow except tibiae distally light brown and tarsomeres brown. Petiole on T1 yellow, contours yellow-brown, and sublateral areas ivory/pale yellow; T2 with median area yellow, adjacent area yellow-brown, and lateral ends yellow; T3 yellow-brown with brown tints; T4 and beyond completely brown; distally each tergum with a narrow hyaline band. In lateral view, T1-3 dark yellow; T4 and beyond yellow, but dorsally yellow-brown. S1-5 yellow; hypopygium yellow-brown. Description. Head ( Fig. 204 A-D ). Head rounded with pubescence long and dense. Proximal three antennal flagellomeres longer than wide (0.19:0.06, 0.20:0.06, 0.20:0.06), antenna longer than body (2.73 [distal antennal flagellomere missing], 2.17); antennal scrobes-frons shallow. Face convex, punctate-lacunose, interspaces wavy and longitudinal median carina present. Frons smooth. Temple wide, punctate-lacunose and interspaces wavy. Inner margin of eyes diverging slightly at antennal sockets; in lateral view, eye anteriorly convex and posteriorly straight. POL shorter than OOL (0.08, 0.11). Malar suture present. Median area between lateral ocelli without depression. Vertex laterally pointed or nearly so and dorsally wide. Mesosoma ( Fig. 204A, F, G, J ). Mesosoma dorsoventrally convex. Distal 1/3 of mesoscutum with lateral margin slightly dented, punctation proximally distinct, but distally with a polished area, interspaces wavy/lacunose. Scutellum triangular, apex sloped and fused with BS , scutellar punctation indistinct throughout, in profile scutellum flat and on same plane as mesoscutum; phragma of the scutellum partially exposed; BS not overlapping the MPM ; ATS demilune with a little, incomplete and parallel carinae only proximally; dorsal ATS groove with carinae only proximally. Transscutal articulation with small and heterogeneous foveae, area just behind transscutal articulation with a smooth and shiny sloped transverse strip. Metanotum with BM upward; MPM circular without median longitudinal carina; AFM with a small lobe and not as well delineated as PFM ; PFM thick, smooth and with a proximal flat flange; ATM completely smooth. Propodeum with indistinct sculpture and without median longitudinal carina, proximal half weakly curved; distal edge of propodeum with a flange at each side and without stubs; propodeal spiracle without distal carina; nucha surrounded by very short radiating carinae. Pronotum with a distinct dorsal furrow, dorsally with a well-defined smooth band; central area of pronotum and dorsal furrow smooth, but ventral furrow with short parallel carinae. Propleuron with a mix of rugae and fine punctation, dorsally with a carina. Metasternum flat or nearly so. Contour of mesopleuron straight/angulate or nearly so; precoxal groove smooth, shiny and shallow, but visible; epicnemial ridge convex, teardrop-shaped. Legs ( Fig. 204A ). Ventral margin of fore telotarsus entire without seta, fore telotarsus almost same width throughout and longer than fourth tarsomere (0.11, 0.08). Hind coxa with punctation only on ventral surface, dorsal outer depression present. Inner spur of hind tibia longer than outer spur (0.25, 0.17), entire surface of hind tibia with dense strong spines clearly differentiated by color and length. Hind telotarsus longer than fourth tarsomere (0.14, 0.11). Wings ( Fig. 204L ). Fore wing with r vein slightly curved; 2RS vein straight; r and 2RS veins forming a weak, even curve at their junction and outer side of junction forming a slight stub; 2M vein straight; distally fore wing [where spectral veins are] with microtrichiae more densely concentrated than the rest of the wing; anal cell 1/3 proximally lacking microtrichiae; subbasal cell with microtrichiae virtually throughout; veins 2CUa and 2CUb completely spectral; vein 2 cu-a present as spectral vein, sometimes difficult to see; vein 2-1A proximally tubular and distally spectral, although sometimes difficult to see; tubular vein 1 cu-a curved and complete, but junction with 1-1A vein spectral. Hind wing with vannal lobe narrow, subdistally and subproximally straightened, and setae present proximally, but absent distally. Metasoma ( Fig. 204A, E, H, I, K ). Metasoma laterally compressed. Petiole on T1 completely smooth and polished, with faint, satin-like sheen, with parallel-sided in proximal half and then narrowing (length 0.35, maximum width 0.15, minimum width 0.09), and with scattered pubescence concentrated in the first distal third. Lateral grooves delimiting the median area on T2 distally losing definition (length median area 0.06, length T2 0.10), edges of median area polished and lateral grooves deep, median area broader than long (length 0.06, maximum width 0.12, minimum width 0.08); T2 with scattered pubescence only distally. T3 longer than T2 (0.20, 0.10) and with scattered pubescence throughout. Pubescence on hypopygium dense. Cocoons. Dark brown oval cocoons adhered to the leaf substrate. Male ( Fig. 205 A-G ). Similar in coloration to female. The head and the legs are missing. Etymology. Stephanie A. Clutts is a research analyst at the University of Kentucky , Lexington, KY, USA . Her primary responsibilities include obtaining and analyzing molecular data related to the revision of Agathidinae wasps ( Braconidae ) and maintaining and distributing samples pertaining to the TIGER project ( Thailand Inventory Group for Entomological Research). Distribution. The parasitized caterpillar was collected in Costa Rica , ACG, Sector San Cristobal (Sendero Vivero), during June 2007 at 730 m in rain forest. Biology. The lifestyle of this parasitoid species is gregarious. Host. Bertholdia albipuncta Schaus ( Erebidae : Arctiinae ) feeding on Guazuma ulmifolia ( Malvaceae ). Caterpillar was collected in fourth instar.