Lasiodiplodia syzygii sp. nov. (Botryosphaeriaceae) causing post-harvest water-soaked brown lesions on Syzygium samarangense in Chiang Rai, Thailand Author Meng, Chao-Rong https://orcid.org/0000-0001-5549-1028 Department of Plant Pathology, Agricultural College, Guizhou University, Guiyang, China Author Zhang, Qian Department of Plant Pathology, Agricultural College, Guizhou University, Guiyang, China Author Yang, Zai-Fu Department of Plant Pathology, Agricultural College, Guizhou University, Guiyang, China Author Geng, Kun Guiyang plant protection and inspection station, Guiyang, China Author Zeng, Xiang-Yu Department of Plant Pathology, Agricultural College, Guizhou University, Guiyang, China Author Thilini Chethana, K. W. https://orcid.org/0000-0002-5816-9269 Center of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai, Thailand & School of Science, Mae Fah Luang University, Chiang Rai, Thailand Author Wang, Yong Department of Plant Pathology, Agricultural College, Guizhou University, Guiyang, China yongwangbis@aliyun.com text Biodiversity Data Journal 2021 2021-01-07 9 60604 60604 http://dx.doi.org/10.3897/BDJ.9.e60604 journal article http://dx.doi.org/10.3897/BDJ.9.e60604 1314-2828-9-e60604 529C29AC5B6A5436A869DD8753970275 Lasiodiplodia syzygii C.R. Meng, Qian Zhang & Yong Wang bis sp. nov. Materials Type status: Holotype . Occurrence: catalogNumber: HGUP 9719 ; recordedBy: Wang Yong ; Taxon: scientificName: Lasiodiplodiasyzygii; kingdom: Fungi; class: Dothideomycetes; order: Botryosphaeriales; family: Botryosphaeriaceae; genus: Lasiodiplodia; Location: country: Thailand ; stateProvince: Chiang Rai; Identification: identifiedBy: Chao-Rong Meng ; dateIdentified: 2020; Record Level: type: ex-type living culture GUCC 9719.1; MFLU 19-0565, isotype, isotype living culture MFLUCC 19-0257. Type status: Other material . Occurrence: catalogNumber: HGUP 9720 and HGUP 9721 ; recordedBy: Wang Yong ; Taxon: scientificName: Lasiodiplodiasyzygii; kingdom: Fungi; class: Dothideomycetes; order: Botryosphaeriales; family: Botryosphaeriaceae; genus: Lasiodiplodia; Location: country: China ; stateProvince: Guiyang; Identification: identifiedBy: Chao-Rong Meng ; dateIdentified: 2020; Record Level: type: living cultures GUCC 9719.2, GUCC 9719.3 and GUCC 9719.4 Description Pathogenic on Syzygium samarangense . Sexual morph : Undetermined. Asexual morph (Fig. 2 ): Conidiomata up to 2 mm diam., pycnidial, covered with hyphae, black, globose, ostiolate, solitary, separate, uniloculate, immersed to semi-immersed. Conidiomatal wall composed of thick-walled, dark brown cells of textura angularis , becoming thin-walled and hyaline towards the inner region. Paraphyses cylindrical, aseptate, hyaline. Conidiophores reduced to conidiogenous cells. Conidiogenous cells 10-14.5 x 3.5-4.5 μm (average = 11 x 3.7 μm , n = 20), hyaline, smooth, holoblastic forming conidia at their tips. Conidia thick-walled, Conidiomatal wall up to 1 μm wide, ovoid with both ends rounded, hyaline and remaining so for a long time, becoming pale brown with obsolete striations and occasionally with 1-septate after discharging from the conidioma, (27-)30-32(-36) x (13-)15-17(-20) μm (average = 31.3 x 16.4 μm , n = 50), L/W = 1.9. Culture characteristics : Conidia germinate on PDA within 24 hours at room temperature (25-30°C) with germ tubes produced from both ends of the conidia. Colonies with white fluffy mycelium on PDA, after 7 days become olivaceous-grey at the centre, white at the edge, raised, fluffy, dense filamentous. Notes Lasiodiplodia syzygii strains are closely related to L. rubropurpurea , but formed a distinct, well-supported clade in the phylogenetic analyses. Base-pairs comparisons between L. syzygii ex-type strain (GUCC 9719.1) and ex-type strain of L. rubropurpurea (WAC 12535) found seven base differences (1.3%) in ITS region and five differences (0.6%) on LSU, but nine differences (2.1%) in tub2 and 34 in TEF1-a (10.4%) (Table 2 ). Lasiodiplodia syzygii produced larger pycnidia (up to 2 mm) and larger conidiogenous cells (10-14.5 x 3.5-4.5 μm ) than L. rubropurpurea (0.5-1.5 mm and 7-13 x 3-5 μm ) ( Burgess et al. 2006 ). Etymology In reference to the host from which the fungus was first isolated.