Taxonomic revision of charon -, floridanum - and muscaeforme - groups of Gryon Haliday, 1833 (Hymenoptera, Scelionidae) from Japan, with descriptions of two new species and host information Author Komeda, Yoto Entomological Laboratory, Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University, Motooka 744, Fukuoka, 819 - 0395, Japan kome123k123@gmail.com Author Mita, Toshiharu https://orcid.org/0000-0001-8322-6045 Entomological Laboratory, Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University, Motooka 744, Fukuoka, 819 - 0395, Japan Author Hirose, Yoshimi Entomological Laboratory, Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University, Motooka 744, Fukuoka, 819 - 0395, Japan Author Yamagishi, Kenzo Entomological Laboratory, Faculty of Agriculture, Kyushu University, Motooka 744, Fukuoka, 819 - 0395, Japan text Journal of Hymenoptera Research 2020 2020-12-29 80 99 135 http://dx.doi.org/10.3897/jhr.80.56178 journal article http://dx.doi.org/10.3897/jhr.80.56178 1314-2607-80-99 F9DF28B5BF7545F5860B3FB234679C6D BCA40A8EFADF5E8A9E51A75C7C3C0D1C 4420570 Gryon yamagishii Mineo, 1981 Figs 1C , 2C , 3C , 4C , 5C, I , 6C Gryon yamagishii Mineo, 1981: Johnson 1992 ; Kononova and Petrov 2002 ; Kononova and Kozlov 2008 . Gryon maruzzae Mineo, 1981: Johnson 1992 ; Kononova and Kozlov 2008 . syn. nov. Gryon sugonjaevi Kozlov & Kononova, 1989: Kozlov and Kononova 1990 ; Johnson 1992 ; Kononova 1995 ; Kononova and Petrov 2002 ; Kononova and Kozlov 2008 . syn. nov. Diagnosis. Frontal depression transversely costate by strong regular carinae. Angular points of occipital carina modified as short sharp horns; horizontal portion of occipital carina straight, expanding inwardly. Coxae brown-black. Description. Female. Length = 1.4-1.6 mm. Color . (Figs 1C , 2C ). Body mainly black. A2-6 brown. A1, mandibles, and legs (excluding coxae) yellow. Head . FCI = 1.16-1.28; LCI = 1.66-1.62; DCI = 2.03-2.23; HW/IOS = 1.64-1.78; head about 1.3 times as wide as mesosoma (HW/TSL = 1.24-1.30). Frons (Fig. 3C ) reticulate with setae; central carina present ventrally; frontal depression weakly developed, transversely costate by strong regular carinae. Vertex reticulate with setae; interocellar space reticulate; hyperoccipital carina absent; POL about 4.6 times as long as OOL (POL/OOL = 3.67-4.83); OOL about 0.4 times as long as LOL (OOL/LOL = 0.33-0.50). Clypeus semi-elliptical. Gena coriaceous with setae; medial genal carina absent. Occiput (Fig. 4C ) transversely costate with setae; occipital carina complete; angular points of occipital carina well-developed, as short sharp horns; horizontal portion of occipital carina straight, expanding inwardly; postoccipital carina discontinuity present; postgena weakly costate along postoccipital carina; postgenal sulcus curved toward hypostoma; postgenal bridge smooth, weakly longitudinally costate beside median sulcus. Antennae (Fig. 5C ) clavate; A1 about 3.5 times longer than radicle, as long as clava; clava with five segments; claval sensilla formula A8-12/2-2-2-2-1; claval length about 3.4 times longer than width. Mandibles thin, tridentate, anterior tooth longer than other teeth. Figure 5. Antennae of Japanese Gryon spp. A G. fulvicoxa sp. nov., female B G. japonicum , female C G. yamagishii , female D G. philippinense , female E G. shisa sp. nov., female F G. pennsylvanicum , female G G. fulvicoxa sp. nov., male H G. japonicum , male I G. yamagishii , male J G. philippinense , male K G. shisa sp. nov., male L G. pennsylvanicum , male. Figure 6. Forewing of Japanese Gryon spp. A G. fulvicoxa sp. nov. B G. japonicum C G. yamagishii D G. philippinense E G. shisa sp. nov. F G. pennsylvanicum . Mesosoma . Cervical pronotal area granulate-punctate with dense setae; epomial carina strongly present, not reaching dorsal edge; pronotal suprahumeral sulcus foveolate, unclear mesad; lateral pronotal area smooth with transverse dense carinae. Propleuron weakly transversely costate. Mesoscutum about 1.4 times as wide as long (TSL/ML = 1.35-1.50), with dense setae, reticulate in anterior, longitudinally costate in posterior; parascutal carina absent; notaulus absent. Mesoscutellum about 2.2 times as wide as long (SW/SL = 2.07-2.35), with dense setae, longitudinally costate mesad, granulate laterad. Mesopleuron smooth with transverse dense carinae above mesopleural canina, rugulose with setae below mesopleural canina; prespecular and upper mesepisternal sulci foveolate; prespecular sulcus with setae; mesopleural carina strongly present; postacetabular sulcus foveolate. Metascutellum weakly produced, longitudinally striate. Metapleuron weakly convex, foveolate-rugulose, with dense setae posteriorly; anterior part of metapleural sulcus and upper paracoxal sulcus with setae. Propodeum foveolate with setae. Fore wing (Fig. 7C ); stigmal vein about 2.5 times longer than marginal vein; postmarginal vein about 3.7 times longer than marginal vein. Metasoma . T1 longitudinally striate, setose laterally. S1 longitudinally striate. T2 longitudinally striate anteriorly, reticulate posteriorly, setose laterally. S2 with setae, granulate mesad, striate laterad. T3 reticulate, with setae laterad and posteriorly. S3-6 punctate with setae. T4 punctate-striate with setae. T5-6 punctate with setae. Male. Almost same as female, but antennae (Fig. 5I ) filiform; A1 yellow, A2-11 brown. Host. Unknown. Biology. In winter, the female overwinters under the bark of Zelkova serrata (Thunberg) Makino ( Ulmaceae ). Material examined. Holotype . G. yamagishii : Kooridono , Ojiya , Niigata Pref . [ Japan : Niigata pref. , Ojiya city, Nishi-Yoshidani, Koridono .] 9.VIII.1970 . K. Yamagishi leg. ♀ [ ELKU ] (Fig. 10 ). G. maruzzae : ( Kyushu ) Okinoshima ( Chikuzen ). [ Japan : Fukuoka pref. , Munakata dist., Munakata town, Chikuzen Oki-no-shima Isl.] 25-28.VII.1958 , Hirashima, Murakami and Y. Miyatake leg. 1♂ [ELKU] (Fig. 11 ). G. sugonjaevi : Yaponiya , Inuyama , 40 km SZ Nagoya . [= Japan : Aichi pref. , Inuyama city.] 4.X.1981 . E. Sugonjaev leg. ♀ [ZIN]. Paratypes . G. yamagishii : same locality as holotype, 1.VIII.1970 . K. Yamagishi leg. ♀ [ELKU]; Nisshin, Aichi-gun, Aichi pref. [ Aichi pref. , Aichi dist. , Nisshin town ], 5.V.1970 . K. Yamagishi leg. ♀ [ELKU]. G. sugonjaevi : Yaponiya , Kaganikhara . [= Japan : Gifu pref. , Kakamigahara city] 19.X.1981 . E. Sugonjaev leg. ♀ [IZAN] . Other material. Japan : Hokkaido , Sapporo city, Toyohira ward, Hitsujigaoka. 43.008°N , 141.415°E ; alt. 100 m , 8-15.VI.2010 , Kazuhiko Konishi leg. (MT) 5♀ [ELKU]; 15-22.VI.2010 , Kazuhiko Konishi leg. (MT) 2♀ [ELKU]; 22-29.VI.2010 , Kazuhiko Konishi leg. (MT) 2♀ [EUMJ]; 29.VI.-6.VII.2010 , Kazuhiko Konishi leg. (MT) 2♀ [EUMJ]; 27.VII.-3.VIII.2010 , Kazuhiko Konishi leg. (MT) 2♀ [EUMJ]; 10-17.VIII.2010 , Kazuhiko Konishi leg. (MT) 2♀ [EUMJ]; Fukushima pref. , Aizu-Wakamatsu city, Monden-machi, Kuroiwa, Minami-Aoki. 6.I.2017 . Keisuke Narita leg. (Collected from under the bark of Zelkova serrata ) 1♀ [ELKU]; Ibaraki pref. , Tsuchiura city (near Tsukuba), Pond Shishituka-Oike . 15.VII.1997 . Victor Fursov leg. 1♀ [IZAN]; Tochigi pref. , Nikko city, Tamozawa. 20-25.VI.2008 . Takeyuki Nakamura leg. (MT) 1♀ [ELKU]; Niigata pref. , Nagaoka city, Urase-machi, 37.464°N , 138.907°E , alt. 40 m , 2015.V.28.-VI. 7 , Ryo Shimizu and So Shimizu leg. (MT), 6♀ [ELKU]; 2015. VI.7.-VI.18 , Ryo Shimizu and So Shimizu leg. (MT), 8♀ [ELKU]; 2015.VII.6-25 , Ryo Shimizu and So Shimizu leg. (MT), 5♂ 17♀ [ELKU]; 2015. VII.25-VIII.7 , Ryo Shimizu and So Shimizu leg. (MT), 2♂ 16♀ [ELKU]; 2015.VIII.7-VIII. 22 , Ryo Shimizu and So Shimizu leg. (MT), 5♂ 2♀ [ELKU]; 2015. VIII.22-IX.5 , Ryo Shimizu and So Shimizu leg. (MT), 7♂ 2♀ [ELKU]; 2015. IX.27-X. 12 , Ryo Shimizu and So Shimizu leg. (MT), 6♀ [ELKU]; 2016.VI.11-23 , Ryo Shimizu and So Shimizu leg. (MT), 3♀ [ELKU]; 2016. VII.13-VIII.1 , Ryo Shimizu and So Shimizu leg. (MT), 2♂ 12♀ [ELKU]; Ishikawa pref. , Mt. Hakusan. 29-21.VIII.1960 . Terunobu Hidaka leg. 1♂ [ELKU]; Hakusan city, San'nomiya , 9-22.VIII.2009 , H. Fukutomi and S. Nakagawa leg. (MT) 1♀ [ELMU]; Nomi city, Mitsukuchi (paddy field) 30.IV-13.V.2011 , H. Fukutomi and R. Ishiguro leg. (MT) 1♀ [ELMU]; 13-26.V.2011 , H. Fukutomi and R. Ishiguro leg. (MT) 3♀ [ELMU]; 9-21.VI.2011 , H. Fukutomi and R. Ishiguro leg. (MT) 3♀ [ELMU]; 17.VII.-4.VIII.2011 , H. Fukutomi and R. Ishiguro leg. (MT) 1♂ [ELMU]; 19.VIII.-7.IX.2011 , H. Fukutomi and R. Ishiguro leg. (MT) 1♂ [ELMU]; 8-21. IX. 2011 , H. Fukutomi and R. Ishiguro leg. (MT) 1♀ [ELMU]; 22.X.-2.XI.2011 , H. Fukutomi and R. Ishiguro leg. (MT) 1♀ [ELMU]; Gifu pref. , Kani city, Katabira, 1-7.V.2004 , K. Ito leg. (MT) 2♀ [ELMU]; 6-12.VIII.2004 , Kenzo Yamagishi leg. (MT) 1♀ [ELMU]; 20-26.VIII.2004 , Kenzo Yamagishi leg. (MT) 1♂ 1♀ [ELMU]; 2-8.X.2004 . Kenzo Yamagishi leg. (MT) 3♀ [ELMU]; Shizuoka pref. , Iwata city, dike of Ohta river, 29.VII.2001 , M. Ura leg. (YPT) 1♀ [IZAN]; 13.VIII.2001 , M. Ura leg. (YPT) 1♂ [IZAN]; 27.VIII.2001 , M. Ura leg. (YPT) 1♂ [ELMU]; 1♂ 1♀ [IZAN]; Aichi pref. , Nagoya city, Chikusa ward, Higashiyama Park. 1-10.V.2001 . M. Watanabe leg. (MT) 1♀ [IZAN]; IV-VI. 1997, Victor Fursov leg. 25♀ [IZAN]; 11.VI.1997 . Victor Fursov leg. 1♂ [IZAN]; Tempaku ward, Yagoto-Urayama, 21.IX.2002 . Kenzo Yamagishi leg. 1♀ [ELMU]; Tempaku ward, 29.IX.2012 . Kenzo Yamagishi leg. 1♀ [ELMU]; Tempaku ward, Meijo University, 17.IV.2013 . N. Kusuda leg. 1♀ [ELMU]; Tempaku ward, Meijo University, 31.VII.2013 . N. Kusuda leg. 1♂ 1♀ [ELMU]; Ichinomiya city, Tomida, Kiso River, 10.V.2013 , Y. Miyata leg. (YPT) 5♀ [ELMU]; 2.VII.2013 , Y. Miyata leg. (YPT) 1♀ [ELMU]; 22.VIII.2013 , Y. Miyata leg. (YPT) 1♀ [ELMU]; Kasugai city, Takagi (Grassland), 14.V.2013 . Y. Kamiya leg. (YPT) 1♀ [ELMU]; 31.VII.2013 . Y. Kamiya leg. (YPT) 1♂ [ELMU]; Nisshin city, Akaike, 30.IX.2006 . Kenzo Yamagishi leg. 1♀ [ELMU]; Nisshin city, Nokata, 28.V-3.VI.2011 . H. Seo and R. Mizutani leg. (MT) 1♀ [ELMU]; 10-17.VI.2011 . H. Seo and R. Mizutani leg. (MT) 1♀ [ELMU]; 2-9.VI.2011 . H. Seo and R. Mizutani leg. (MT) 1♀ [ELMU]; [ELMU]; 16-22.VI.2011 . H. Seo and R. Mizutani leg. (MT) 1♂ [ELMU]; Toyota city, Obara, Kajiyashiki-cho , Nishinohara, 12.VI.-6. 2014. Hiroaki Iketake leg. (MT) 1♀ [ELKU]; 12-26.X.2014 . Hiroaki Iketake leg. (MT) 1♀ [ELKU]; Osaka pref. , Sen'nan Dist., Misaki town, Kyoshi , 16.IV.1975 . 3♀ [ELKU]; Wakayama pref. , Wakayama city, Sando , 16.IV.1975 . 1♀ [ELKU]; Shimane pref. , Izumo city, Mt. Kita-yama, 11.VI.1969 . Minoru Miyazaki leg. 1♀ [ELKU]; Tokushima pref. , Zen'nyuji-toh (River island in Yoshino River), 13-22.V.2003 . K. Ohara and H. Otsuka leg. (MT) 3♀ [ELMU]; 5-17.IX.2003 . K. Ohara and H. Otsuka leg. (MT) 3♀ [ELMU]; Ehime pref. , Matsuyama city, Sugitake. 26.V.1976 . N. Takaki leg. 1♀ [EUMJ]; Matsuyama city, Komenono. 1.VI.1978 . N. Takaki leg. 1♀ [EUMJ]; Matsuyama city, Jikiba-cho . 33.51°N , 132.42°E ; 14.V.2016 . K. Kuroda and K. Sogoh leg. 1♀ [EUMJ]; Matsuyama city, Suemachi, Wakigafuchi Park. 33.52°N , 132.49°E ; 20.V.2017 . K. Kuroda leg. 1♀ [EUMJ]; Ochi dist., Kamijima town, Iwagi, Akahone Isl., 29-30.XI.2004 . Jiro Ogawa leg. 1♀ [EUMJ]; Kami-Ukena dist., Yanadani vill., Yokono (alt. 650 m ). 7.V.1994 . M. Sakai leg. 1♀ [EUMJ]; Kami-Ukena dist., Kuma-Kougen town, Saragamine. 4.VI.2006 . Yugo Sato leg. 1♀ [EUMJ]; Kami-Ukena dist., Kuma-Kougen town, Yurano. 5.VI.2007 . Eiji Yamamoto leg. 1♀ [EUMJ]; 17.VII.2007 . Eiji Yamamoto leg. 1♀ [EUMJ]; 24-26.VII.2008 . Eiji Yamamoto leg. 1♀ (MT) [EUMJ]; Kita dist., Uchiko town, Hongawa, Hirose shrine. 21.V.2014 . Eiji Yamamoto leg. 2♀ [EUMJ]; Kita dist., Uchiko town, Hiraoka. 11-15.V.2014 . Eiji Yamamoto leg. (MT) 1♀ [EUMJ]; 16-20.V.2014 . Eiji Yamamoto leg. (MT) 2♀ [EUMJ]; Kochi pref. , Nankoku city, Estuary of Monobe river. 1.VII.2002 . M. Sakai leg. 1♀ [EUMJ]; Kami city, Kami-anauchi. 30-31.VII.2016 . Kazuhiko Konishi leg. (MT) 1♀ [EUMJ]; Hata dist., Nishi-Tosa vill., Oku-Yanai, Kuroson, 29.IV.1956 , Yozo Murakami leg., 2♀ [ELKU]; Fukuoka pref. , Fukuoka city, Mt. Tachibana-yama, 14.V.1970 . Minoru Miyazaki leg. 3♀ [ELKU]; 13.V.1994 . Hiroshi Honda leg. (YPT) 1♀ [ELKU]; 2.VII.1994 . Hiroshi Honda leg. (YPT) 5♂ [ELKU]; 16.VII.1994 . Hiroshi Honda leg. (YPT) 2♂ 4♀ [ELKU]; 23.VII.1994 . Hiroshi Honda leg. (YPT) 1♂ 1♀ [ELKU]; 30.VII.1994 . Hiroshi Honda leg. (YPT) 1♀ [ELKU]; 7.VIII.1994 . Hiroshi Honda leg. (YPT) 1♂ 1♀ [ELKU]; Fukuoka city, Mt. Abura-yama, 21.VI.1959 . Shoichi Miyamoto leg. 1♀ [ELKU]; Fukuoka city, East ward, Tatara. 8.IX.1996 . Yoshimitsu Higashiura leg. 1♀ [ELKU]; Fukuoka city, Sawara ward, Mt. Sefuri-san, 1.VIII.1992 , Yoshimitsu Higashiura leg. 2♀ [ELKU]; Kasuya dist., Sasaguri town, Mt. Wakasugi-yama. 12.V.1969 . Masako Honda leg. 1♀ [ELKU]; 13.V.1970 . Masako Honda leg. 1♀ [ELKU]; Dazaifu city, Kitadani. 5.III.2016 . Yu Hisasue leg. 1♀ [ELKU]; Iizuka city, Ae, 33.564°N , 130.640°E , 5.V.2018 . Yu Hisasue leg., (collected from blossoms of Acer palmatum ) 1♀ [ELKU]; Tagawa dist., Soeda town, Mt. Hiko-san. 28.IX.1966 , Masako Honda leg. 3♀ [ELKU]; 8.V.1967 , Masako Honda leg. 1♀ [ELKU]; 10.V.1967 , Masako Honda leg. 2♀ [ELKU]; 20.IV.1967 , Kenkichi Kanmiya leg. 1♀ [ELKU]; 20.VI.1967 , Kenkichi Kanmiya leg. 1♀ [ELKU]; 20.IX.1967 , Kenkichi Kanmiya leg. 3♀ [ELKU]; 25.VII.196-, Koichi Takeno leg. (MT) 1♂ [ELKU]; 5.VI.1970 , Kenkichi Kanmiya leg. 3♀ [ELKU]; 15.IV.1971 , Michitaka Chujo leg. 1♀ [ELKU]; 24.VII.1972 , Michitaka Chujo leg. 1♀ [ELKU]; 22-26.V.1979 , Kaoru Maeto leg. 1♀ [NIAES]; 25-26.VII.1979 , Kaoru Maeto leg. 3♂ 1♀ [NIAES]; 24-26.X.1979 , Kaoru Maeto leg. 3♀ [NIAES]; Miyako dist., Saigawa town, Hobashira, Mt. Hiko-san, No-toge pass. 29.VIII.1993 , N. Wasano leg. 1♂ [ELKU]; Saga pref. , Kishima dist., Yamauchi town. 22.X.1991 . Yoshimitsu Higashiura leg. 2♀ [ELKU]; Higashi-Matsuura dist., Genkai town, Kariya. 23.VIII.1993 . Yoshimitsu Higashiura leg. 3♀ [ELKU]; Nagasaki pref. , Minami-Takaki dist., Obama town. 25.II.1960 . Terunobu Hidaka leg. 1♀ [ELKU]; Kumamoto pref. , Hondo city, Nishinoku Lake, 5.VI.1999 . Victor Fursov leg. 1♀ [IZAN]; Miyazaki pref. , Higashi-Usuki dist., Shiiba vill., Shimo-Fukura, Ohira. 29.VIII.1993 , N. Wasano leg. 3♀ [ELKU]; Kagoshima pref. , Aira dist., Kirishima town, Taguchi, Kirishima-jingu shrine, 15.VII.1969 . Osamu Yata leg. 1♂ [ELKU]; Kimotsuki dist., Sata town, Magome, Tajiri, Kape Sata, 17.VII.1969 . Osamu Yata leg. 2♀ [ELKU]; Kumage Dist., Yaku-shima Isl., Yaku town, Kurio, 8.VII.1975 . Kenzo Yamagishi leg. 2♂ 1♀ [ELKU]; Kagoshima Dist. , Toshima vill., Tokara Isles., Kuchi-no-shima Isl., 2.V.1993 , Hiroshi Honda leg., 2♀ [ELKU]; Satoshi Kamitani leg., 2♀ [ELKU]; Oshima dist., Amami-oshima Isl., Uken vill., Mt. Yuwan-dake, 30.VII.1963 , J. L. Gressitt leg. 1♂ 1♀ [ELKU]; Okinawa pref. , Okinawa Isl., Nago city, Mt. Nago-dake, 26.587°N , 128.000°E , 140 m , 3-14.VII.2016 , Keisuke Narita leg. 1♀ [ELKU]; Tokyo Met., Hachijo Isl., Hachijo town, Kamogawa. 27.V.1964 , Y. Hirashima and M. Shiga leg. 3♀ [ELKU]; Hachijo town, Mitsune-Kantoyama. 30.V.1964 , Y. Hirashima and M. Shiga leg. 1♀ [ELKU]; Fukuoka pref. , Munakata dist., Munakata town, Chikuzen Oki-no-shima Isl. 25-28.VII.1958 , Yoshihiro Hirashima, Yozo Murakami and Yorio Miyatake leg. 1♂ [ELKU]. Distribution. Japan (Hokkaido; Honshu: Fukushima, Ibaraki, Tochigi, Niigata, Ishikawa, Gifu, Shizuoka, Aichi, Osaka, Wakayama, and Shimane; Shikoku: Tokushima, Ehime, and Kochi; Kyushu: Fukuoka, Saga, Nagasaki, Kumamoto, Miyazaki, and Kagoshima; Ryukyus: Yaku-shima Isl., Naka-no-shima Isl., Amami-ohshima Isl., and Okinawa Isl.; Hachijo Isl; and Chikuzen Oki-no-shima Isl.) Remarks. Among Japanese species, G. yamagishii is similar to G. japonicum and G. fulvicoxa , but G. yamagishii differs from these species in the sculpture of the antennal depression ( G. yamagishii (Fig. 3C ): transversely costate by strong regular carinae; G. fulvicoxa (Fig. 3A ) and G. japonicum (Fig. 3B ): transversely costate by strong irregular carinae) and the presence of the short sharp horn on the angular point of occipital carina (Fig. 4C ). Russian Far Eastern species, G. amissum Kozlov & Kononova, 1990 and G. sibiricum Kononova, 2001 are also similar to G. yamagishii in the sculpture of the frons. In G. amissum and G. sibiricum , however, the angular points of occipital carina are not developed as horns, and the mesopleural carina is weak ( G. amissum ) or absent ( G. sibiricum ). We examined the holotype of G. maruzzae at ELKU (Fig. 11 ) and G. sugonjaevi at ZIN, and they belong to G. yamagishii .