New records of 24 ciliate species (Protozoa, Ciliophora) collected in South Korea
Author
Kim, Ji Hye
Natural Science Research Institute, Gangneung-Wonju National University, Gangneung, Republic of Korea
Author
Jung, Ji Hye Moon and Jae-Ho
text
Journal of Species Research
2018
7
4
291
314
journal article
10.12651/JSR.2018.7.4.291
2713-8615
13163147
5.
Sterkiella tricirrata
(Buitkamp, 1977)
Berger, 1999
(
Table 1
,
Fig. 5
)
Material examined.
Terrestrial
moss collected from
Gagye Shore
,
Geumgye-ri
,
Gogun-myeon
,
Jeollanamdo
,
Korea
(
34°25
ʹ
42
ʺ
N
,
126°21
ʹ
05
ʺ
E
) on
July 7, 2016
.
Diagnosis.
Body size 85-100 × 35-45 μm
in vivo
, 57- 90 × 24-45 μm after protargol impregnation; elliptical shape; colorless; one contractile vacuole on left side of mid-body; cortical granule absent; 2 macronuclear nodules and 1-4 micronuclei; 22-29 adoral membranelles; 3 frontal cirri; 1 buccal cirrus; 4 frontoventral cirri; 3 postoral ventral cirri; 2 pretransverse ventral cirri; 3 transverse cirri; 15-21 left and 18-23 right marginal cirri; 6 dorsal kineties and 4 caudal cirri.
Fig. 4.
Australocirrus aspoecki
in vivo
(A, B) and after protargol impregnation (C). A. Living cell to show body shape. B. Reddish cortical granules and dorsal brush. C. Ventral view. Scale bars = 100 μm (A); 50 μm (C); 10 μm (B).
Table 1.
Morphometry of
Sterkiella tricirrata
.
| Characteristics a |
mean |
M |
SD |
SE |
CV |
Min |
Max |
n |
| Body, length |
69.3 |
69 |
8.6 |
2.0 |
12.3 |
57 |
90 |
19 |
| Body, width |
32.6 |
32 |
4.7 |
1.1 |
14.5 |
24 |
45 |
19 |
| Body length:width, ratio |
2.14 |
2.11 |
0.21 |
0.05 |
9.6 |
1.78 |
2.50 |
19 |
| Adoral zone, length |
23.6 |
23.0 |
1.48 |
0.34 |
6.30 |
21 |
26 |
19 |
| Body length:adoral zone length, ratio |
2.93 |
2.87 |
0.27 |
0.06 |
9.2 |
2.58 |
3.60 |
19 |
| Largest adoral membranelle, width |
5.0 |
5.0 |
0.12 |
0.03 |
2.28 |
5.0 |
5.5 |
19 |
| Adoral membranelles, number |
24.2 |
24.0 |
1.70 |
0.40 |
7.0 |
22 |
29 |
18 |
| First macronuclear nodule, length |
9.6 |
9.0 |
2.04 |
0.47 |
21.3 |
7.0 |
16.0 |
19 |
| First macronuclear nodule, width |
6.7 |
7.0 |
0.67 |
0.15 |
10.0 |
5.0 |
8.0 |
19 |
| Macronuclear nodules, number |
1.95 |
2.0 |
0.23 |
0.05 |
11.8 |
1 |
2 |
19 |
| First micronucleus, diameter |
1.66 |
1.50 |
0.24 |
0.06 |
14.4 |
1.50 |
2.00 |
19 |
| Micronuclei, number |
2.16 |
2.00 |
0.60 |
0.14 |
27.9 |
1 |
4 |
19 |
| Frontal cirri, number |
3 |
3 |
0 |
0 |
0 |
3 |
3 |
18 |
| Buccal cirrus, number |
1 |
1 |
0 |
0 |
0 |
1 |
1 |
19 |
| Frontoventral cirri, number |
4 |
4 |
0 |
0 |
0 |
4 |
4 |
19 |
| Postoral ventral cirri, number |
3 |
3 |
0 |
0 |
0 |
3 |
3 |
19 |
| Pretransverse ventral cirri, number |
2 |
2 |
0 |
0 |
0 |
2 |
2 |
19 |
| Transverse cirri, number |
3 |
3 |
0 |
0 |
0 |
3 |
3 |
19 |
| Right marginal cirri |
20 |
20 |
1.33 |
0.31 |
6.7 |
18 |
23 |
19 |
| Left marginal cirri |
18.1 |
18 |
2.26 |
0.52 |
12.5 |
15 |
21 |
19 |
| Dorsal kineties, number |
6 |
6 |
0 |
0 |
0 |
6 |
6 |
17 |
| Dorsal bristles in dorsal kinety 1, number |
17.7 |
17 |
1.45 |
0.37 |
8.2 |
15 |
21 |
15 |
| Caudal cirri, number |
3 |
3 |
0 |
0 |
0 |
3 |
3 |
19 |
a
Data based on protargol preparations; all measurement in μm. CV = coefficient of variation (%); M = median; Max= maximum; mean = arithmetic mean; Min = minimum; n = number of specimens examined; SD= standard deviation; SE = standard error of arithmetic mean
Morphogenesis.
Oral primordium develops slightly above the leftmost transverse cirrus. Origin of the frontal-ventral-transverse primordia as follows: for proter,
Fig. 5.
Sterkiella tricirrata
in vivo
(A-C), protargol impregnated specimen (D-L) and morphogenetic stages (E-L). A. Typical individual to show ellipsoidal body shape (arrowhead indicates contractile vacuole). B. Transverse cirri (arrow). C. Dorsal bristles (arrowheads). D. Ventral view. E. Ventral view of early divider; asterisk indicates a very early divider to show oral primordium. F, H, J. Ventral views of middle dividers. G, I. Dorsal views of middle dividers. K. Ventral view of late divider. L. Dorsal view of late divider. Scale bars =20 μm.
primordium I from undulating membranes, primordium II from cirrus II/2, primordium III from cirrus III/2?, primordium IV from cirrus IV/3?, primordium V from cirrus IV/3?, primordium VI from de novo?; for opisthe, primordia I-III from oral primordium, primordium IV from cirrus IV/2?, primordium V from cirrus V/4, primordium VI from cirrus V/4. Both primordia V of the two daughters are connected in a middle stage. Dorsal ciliature follows typical
Oxytricha
pattern.
Distribution.
Cote d’Ivoire
,
Korea
.
Remarks.
Sterkiella tricirrata
(Buitkamp, 1977)
Berger, 1999
is a poorly described species that has three transverse cirri. Kumar
et al
. (2015) considered that
S. tricirrata
and
S. histriomuscorum
could be separated from
Sterkiella
based on the decreased number of transverse cirri. However, the morphology and molecular data of
S. tricirrata
are not enough to clarify this issue.
Sterkiella tricirrata
is distinguishable from
S. histriomuscorum
in the number of transverse cirri (3 vs. 4 or 5) (
Berger, 1999
).
Voucher slides.
Two slides of protargol-impregnated specimens were deposited at National Institute of Biological Resources,
Korea
(NIBRPR0000107896, NIBRPR0000107897).