Limosilactobacillus balticus sp. nov., Limosilactobacillus agrestis sp. nov., Limosilactobacillus albertensis sp. nov., Limosilactobacillus rudii sp. nov. and Limosilactobacillus fastidiosus sp. nov., five novel Limosilactobacillus species isolated from the vertebrate gastrointestinal tract, and proposal of six subspecies of Limosilactobacillus reuteri adapted to the gastrointestinal tract of specific vertebrate hosts Author Li, Fuyong Author Cheng, Christopher C. Author Zheng, Jinshui Author Liu, Junhong Author Quevedo, Rodrigo Margain Author Li, Junjie Author Roos, Stefan Author Gänzle, Michael G. Author Walter, Jens text International Journal of Systematic and Evolutionary Microbiology 2021 004644 2021-02-01 71 2 1 21 http://dx.doi.org/10.1099/ijsem.0.004644 journal article 10.1099/ijsem.0.004644 6b321035-fd9c-4b25-a3b6-79a05a309793 1466-5034 PMC8346765 33533708 6048739 DESCRIPTION OF LIMOSILACTOBACILLUS ALBERTENSIS SP. NOV. Limosilactobacillus albertensis (al.ber.ten′ sis. N.L. masc. adj. albertensis , pertaining to Alberta , a province of Canada where the isolates were characterized and identified). Cells are Gram-positive , non-motile, non-spore forming, catalase-negative and heterofermentative. Cells are rodshaped, measuring 0.8–2.4×0.6–1.2 µm. Colonies of the type strain Lr 3000 T on MRS agar plate incubated at the anaerobic condition at 37 °C for 2 days are whitish, opaque, raised, circular and entire, with a diameter of 0.8–1.5 mm ; colonies on MRS agar plate at the aerobic condition show similar morphological characteristics as colonies on MRS agar incubated anaerobically, but with a smaller diameter of 0.5–1 mm . D-Lactate, L-lactate and gas are produced from glucose fermentation by the type strain Lr 3000 T . Cell growth occurs at 30, 37 and 45 °C (optimum), but not at 15 ° C. Growth occurs at pH 4.0–8.0 in MRS broth. The major fatty acids of Lr 3000 T are summed feature 7 (combination of C 19:1 ω 6 c and/or C 19:0 cyclo ω 10 c ) and C 18:1 ω 9c, followed by C 16:0 . Acid is produced from L-arabinose,D-ribose,D-xylose, D-galactose, D-glucose, methyl α- D-glucopyranoside, aesculin, maltose, lactose, melibiose, sucrose and raffinose; the fermentation of potassium gluconate is strain-specific; acid is not produced from D-fructose,D-mannose, glycerol, erythritol, D-arabinose, L-xylose, D-adonitol, methyl β - Dxylopyranoside , L-sorbose,L-rhamnose, dulcitol, inositol, D-mannitol, D-sorbitol, methyl α- D-mannopyranoside, N -acetylglucosamine, amygdalin, arbutin, salicin, cellobiose, trehalose, inulin, melezitose, starch, glycogen, xylitol, gentiobiose, turanose, D-lyxose, D-tagatose, D-fucose, L-fucose,D-arabitol,L-arabitol, potassium 2-ketogluconate or potassium 5-ketogluconate. The cell-wall peptidoglycan of Lr 3000 T contains the amino acids alanine (Ala), glutamic acid (Glu), lysine (Lys) and aspartic acid (Asp), with the molar ratio 1.1 (Ala):0.8 (Asp):1.0 (Glu):1.0 (Lys), suggesting the cell-wall peptidoglycan type A4α L-Lys– D-Asp [ 38 ]. The DNA G+C content of Lr 3000 T is 38.8mol%. The type strain, Lr 3000 T (=DSM 110573 T = LMG 31632 T ), was isolated from the stomach of a hamster in the USA .