A new species of Miogryllus Saussure, 1877 and new record of Miogryllus piracicabensis Piza, 1960 (Orthoptera: Gryllidae) from State of Rio Grande do Sul, Brazil, with calling song and chromosome complement Author Orsini, Marcelo Pinheiro Author Costa, Maria Kátia Matiotti Da Author Szinwelski, Neucir Author Martins, Luciano De Pinho Author Corrêa, Robson Crepes Author Timm, Vitor Falchi Author Zefa, Edison text Zootaxa 2017 4291 2 361 372 journal article 32681 10.11646/zootaxa.4291.2.8 ea4c3cf5-f543-4665-ba47-03423b1c57a4 1175-5326 829689 380EF354-32A9-4643-9FC1-4B1E65601E6B Miogryllus piracicabensis Piza, 1960 Miogryllus piracicabensis Piza, 1960 . Studia Entomolica, 3, 253–56. Type locality: Brazil , State of São Paulo , municipality of Piracicaba. Examined Material . Brazil , State of Rio Grande do Sul , municipality of Capão do Leão (31o48’1.354’S, 52o25’5.833”W ), 38 adult males, 9 adult females ( MZUSP ), October to November, 2007 and from October to April, 2015-2016 , E. Zefa & M. P. Orsini , leg . External morphology ( Figs 21 , 23–28 ) . Similar to M. itaquiensis n. sp. , differing in the following characteristics: head slightly wider than pronotum; ecdysial suture whitish; presence of a diagonal line in the fifth palpomere slightly darkened; mandible light brown; considering 38♂♂ and 9♀♀ , the marks in the pronotum lateral lobe vary in the color pattern, with 75.6% of the individuals showing these mark entirely whitish, 15.6% anteroventral whitish and light brown posteriorly, 6.7% antero-ventral light brown and whitish posteriorly ( Fig. 6 ), and 2.1% entirely light brown; outer auditory tympanum always present, bigger than occurs in M. itaquiensis n. sp. ; inner auditory tympanum varying as 56% absent, 40% very smaller than outer or vestigial, 4% one third smaller than outer; subapical spurs number varying from 5 to 7 outer and from 4 to 6 inner. Phallic sclerites ( Figs 29–31 ) . Idem M. itaquiensis n. sp. including same variations. Right tegmen ( Fig. 32 , n=41). Similar to M. itaquiensis n. sp. , however apical region is reduced. Measurements: harp area, 5.4 ± 0.72mm 2 (3.7–6.8); mirror area, 2.1 ± 0.38mm 2 (1.3–3); dorsal field length, 6.7 ± 0.7mm (4.9–7.9); dorsal field width, 2.9 ± 0.4mm (2.3–4,6); lateral field length, 6.5 ± 0.6mm (5.2–7.9); lateral field width, 2.6 ± 0.36mm (1.8–3.7); file length, 1.8 ± 0.17mm (1.3–2.1); teeth number, 97.5 ± 5.2 teeth (87–106); six accessory veins in lateral field. Calling song ( Figs 19–20 , Tab. 1 ) . Calling song emitted only in chirps; 4 to 6 pulses per chirp (percentage in the Table 1 ); chirp rate, 4.8 ± 0.65s (3.9–6, n = 30); chirp period, 0.20 ± 0.026s (0.17–0.25, n = 30); inter-chirp interval, 0.13 ± 0.03s (0.09–0.17, n = 30); dominant frequency 5910 ± 188Hz (5618–6134, n = 30); temperature from 17 to 25°C. FIGURES 23–28. Male morphological characteristics of Miogryllus piracicabensis Piza, 1960 . 23, 24, 25—habitus after alcohol fixation, in dorsal, ventral and lateral views, respectively; 26—head, lateral view; 27—supranal plate; 28—subgenital plate. FIGURES 29–34. Genitalia, right tegmen and ovipositor of Miogryllus piracicabensis Piza, 1960 . 29—31—phallic sclerites, dorsal, ventral and lateral view, respectively; 32—male right tegmen; 33—supranal plate, cerci and ovipositor; 34—subgenital plate, cerci and ovipositor. Conventions: MLPs—Main Lobe of Pseudepiphallus; Ps.Ap.—Pseudepiphallic Apodeme; Ect.F— Ectophallic Fold; Arc—Ectophallic Arc; Ps.P— Pseudepiphallus Paramere; Ps.Ap—Pseudepiphallic Apodeme; End.C— Endophallic Cavity; R—Ramus. FIGURES 35–36. Karyotype of Miogryllus . 35— Miogryllus itaquiensis n. sp. ; 36— Miogryllus piracicabensis Piza, 1960 . TABLE 1. Calling song parameters of Miogryllus piracicabensis Piza, 1960 . 1–4=chirp duration with three, four, five and six pulses, respectively; 5=chirp rate; 6=chirp period; 7=inter-chirp interval; 8=dominant frequency (Hz); 9=Temperature.

1	2	3	4	5	6 (n = 30)	7 (n = 30)	8	9
02DA	0.05 ± 0.001 (0.04–0.05) n = 34, 74%	0.06 ± 0.002 (0.06–0.07) n = 12, 26%	–	–	4.6	0.2 ± 0.016 (0.18–0.25)	0.16 ± 0.016 (0.13–0.2)	5860	18°C
07DA	–	0.06 ± 0.002 (0.05–0.07) n = 32, 60.4%	0.07 ± 0.001 (0.07–0.08) n = 21, 39.6%	–	5.3	0.1 9 ± 0.013 (0.16–0.22)	0.13 ± 0.016 (0.10–0.17)	5793	25°C
17DA	–	–	0.07 ± 0.001 (0.07–0.07) n = 34, 56.7%	0.09 ± 0.001 (0.08–0.09) n = 26, 43.3%	6.0	0.17 ± 0.008 (0.16–0.19)	0.09 ± 0.009 (0.07–0.11)	6082	21°C
18DA	–	0.06 ± 0.001 (0.057–0.059) n = 3, 6.4%	0.07 ± 0.001 (0.07–0.07) n = 43, 93.6%	–	4.6	0.2 2 ± 0.047 (0.17–0.45)	0.14 ± 0.015 (0.12–0.19)	6065	19°C
21DA	–	–	0.08 ± 0.07 (0.08–0.002) n = 51, 100%	–	5.1	0.1 9 ± 0.008 (0.17–0.21)	0.11 ± 0.008 (0.09–0.123)	6134	21°C
29DA	–	0.06 ± 0.001 (0.061–0.063). n = 4, 10%	0.08 ± 0.002 (0.077–0.084) n = 36, 90%	–	4.0	0.2 5 ± 0.054 (0.19–0.43)	0.17 ± 0.054 (0.11–0.36)	5770	18°C
MMC0 0 6	–	0.06 ± 0.003 (0.056–0.057) n = 3, 5.9%	0.07 ± 0.0008 (0.07–0.074) n = 48, 94.1%	–	4.8	0.1 9 ± 0.02 (0.16–0.24)	0.13 ± 0.02 (0.1–0.17)	5611	23°C

Female ( Figs 22 , 33–34 ). Body shape and color pattern similar to males, slightly bigger than male; short tegmina, covering two first abdominal tergites; subgenital plate trapezoid; ovipositor slightly longer than femur III, apex lanceolated. Measurements (mm). Male (n=38): body length, 15.2 ± 1.44 (11.9–18); pronotum width, 4.3 ± 0.27 (3.6–5); pronotum length, 2.3 ± 0.18 (1.8–2.7); head width, 4.2 ± 0.3 (3.4–4.9); interocular distance, 3.3 ± 0.33 (2.5–3.9); femur III length, 8.8 ± 0.61 (7.6–10.3); tibia III length, 5.9 ± 0.41 (5.2–6.9). Female (n = 9): body length, 16.5 ± 2.64 (13.6–20.1); pronotum width, 4.8 ± 0.36 (4.2–5.2); pronotum length, 2.7 ± 0.26 (2.4–3.1); head width, 44.6 ± 0.24 (4.2–4.8); interocular distance, 3.4 ± 0.3 (3.0–3.8); femur III length, 9.7 ± 0.5 (9.1–10.6); tibia III length, 6.4 ± 0.38 (5.6–7.0); dorsal field length, 3.3 ± 0.6 (2.1–4.2); dorsal field width, 2.0 ± 0.3 (1.4–2.6); ovipositor length, 11.5 ± 1.6 (8.8–13.6). Karyotype ( Fig. 36 ) . Diploid chromosome number of 2n = 24 + X ♂ = 25, and 2n = 24 + XX ♀ = 26; three pairs of metacentric chromosomes (pair 1, ci = 43.1, with secondary constriction in p arm, which left a small satellite at the chromosome tip; pair 2, ci = 44.7; pair 4, ci = 44.4), five pairs of submetacentric (pair 3, ci = 34.2; pair 5, ci = 15.1; pair 6, ci = 29; pair 7, ci = 14.8; pair 10, ci = 17.1) and four pairs of acrocentric (pair 8, ci = 10.3; pair 9, ci = 11.5; pair 11, ci = 11.1; pair 12, ci = 10). The X chromosome is metacentric (ci = 43.4), and the largest of the complement.