Macrobiotus (Eutardigrada, Macrobiotidae) from the Great Smoky Mountains National Park, Tennessee / North Carolina, USA (North America): two new species and six new records Author Bartels, Paul J. Author Pilato, Giovanni Author Lisi, Oscar Author Nelson, Diane R. text Zootaxa 2009 2022 45 57 journal article 10.5281/zenodo.186100 06b79f4e-5e07-4315-9636-eb8a036e3172 1175-5326 186100 Macrobiotus halei sp. nov. Figs. 4–7 Type locality: Brushy Mountain ATBI Plot ( W 83o 25.9307’ , N 35o 40.5596’ , 1468 m asl), GSMNP , Sevier County, Tennessee, USA . In soil and leaf litter in a heath bald community (dominant species Rhododendron maximum and Kalmia latifolia ). Material examined: Holotype : Brushy Mountain. Paratypes : Brushy Mountain: 2 adults and 3 eggs (2 with fully developed embryos); Cataloochee: 7 specimens and 1 egg (with fully-developed embryo). Species diagnosis: Colorless; cuticle with very small tubercles, quite difficult to see; very fine granules present on all legs, more evident on legs IV; eyes absent. Buccal armature in oral cavity of richtersi - type ; buccal tube wide; stylet supports inserted on the buccal tube at 81.0–82.7% of its length; three macroplacoids and a microplacoid (far away from the third macroplacoid) present. Claws of the hufelandi - type ; accessory points and lunules present. Eggs with truncated conical processes with a reticular design; egg shell areolated; areolae sculptured. Description of the holotype : Body length 504.0 µm; colorless; eyes absent; cuticle with very small circular or elongated tubercles, more visible on the dorsal and lateral body wall, but very difficult to see ( Fig. 4 A, B); smaller granules present on all legs ( Fig. 4 B), but more evident on hind legs, especially in larger animals. Mouth terminal with 10 peribuccal lamellae; buccal armature in oral cavity well-developed ( Fig. 4 C) with anterior band of small teeth, posterior band of triangular teeth (some of them fused), and three dorsal and three ventral transverse ridges. Some supplementary teeth present between the ring of triangular teeth and the transverse ridges on the ventral wall of the buccal cavity. Buccal tube 62.3 µm long and 17 µm wide ( pt = 27.3); stylet supports inserted on the buccal tube at 81.6% of its length ( pt = 81.6). Pharyngeal bulb with apophyses, three rod-shaped macroplacoids and a small microplacoid (with antero-lateral wings) far away from the third macroplacoid, a characteristic typical of species in the richtersi group. First macroplacoid 10.9 µm long ( pt = 17.5), the second 7.8 µm ( pt = 12.5), the third 12.1 µm ( pt = 19.4), the microplacoid 3.9 µm ( pt = 6.3); entire placoid row 47.9 µm ( pt = 76.9); macroplacoid row 37.1 µm long ( pt = 59.6). Claws, of the hufelandi - type , well-developed ( Fig. 4 D, E), with evident accessory points on the main branches. Internal and external claws on the third pair of legs 13.5 µm long ( pt = 21.7) and 14.7 µm long ( pt = 23.6), respectively; anterior and posterior claws on the hind legs 16.3 µm long ( pt = 26.2) and 17.4 µm long ( pt = 27.9), respectively. Other claws not measurable due to their unfavorable orientation. Eggs, laid freely, spherical with truncated conical processes ( Fig. 5 ). In two measured eggs the diameter is 91.0 and 96.0 µm, excluding the processes, and 117.0 and 121.0 µm including them; 11 processes present around the circumference, 24–27 in the hemisphere. Processes 11.0–14.0 µm high, with basal diameter 22.0–23.5 µm. Processes with dense reticular design with elongated meshes having sinuous margins on the basal portion, almost isodiametric on the distal portion ( Fig. 5 B). Egg shell areolated between processes. The large areolae, with thick margins, have a more or less evident thickened central portion with circular light spots forming an irregular reticular design ( Fig. 5 C) (similar to pits in a strawberry). The paratypes are similar to the holotype in both qualitative and metric characters. Measurements and pt values of selected morphological structures for the smallest and largest examined specimens are given in Table 2 . TABLE 2. Macrobiotus halei sp. nov. metric characters (all values in µm, pt ratios in brackets).
smallest specimen largest specimen
Body length 405.0 630.0
Buccal tube length 53.5 66.8
Buccal tube width 14.2 [26.5] 19.1 [28.6]
Stylet supports 43.6 [81.5] 54.4 [81.5]
Placoid row 36.9 [69.0] 49.6 [74.3]
Macroplacoid row 27.8 [52.0] 37.0 [55.4]
First macroplacoid 9.2 [17.2] 12.4 [18.6]
Second macroplacoid 6.4 [12.0] 8.8 [13.2]
Third macroplacoid 9.8 [18.3] 12.7 [19.0]
Microplacoid 3.4 [6.4] 4.0 [6.0]
Internal claw II 10.9 [20.4] 14.1 [21.1]
External claw II 12.2 [22.8] 15.5 [23.2]
Internal claw III 11.6 [21.7] 14.6 [21.9]
External claw III 12.6 [23.6] 16.3 [24.4]
Anterior claw IV 13.0 [24.3] 17.5 [26.2]
Posterior claw IV 15.0 [28.0] 19.1 [28.6]
Etymology: The name ‘ halei ” is in honor of Mr. Gilbert Hale in appreciation of his long-time service as lab assistant for Dr. Diane Nelson. He has produced thousands of slides for the tardigrade inventory of the ATBI, and he is exceptional at finding eggs. Gilbert is a “mighty bear hunter”. FIGURE 4. Macrobiotus halei sp. nov. (holotype). A, detail of the cuticular ornamentation (arrow). B, detail of the ornamentation on a leg where small tubercles and very small dots are visible. C, buccal-pharyngeal apparatus. D, claws of the third pair of legs. E, claws of the fourth pair of legs. (Scale bars = 10.0 µm) Type depositories: The holotype ( USNM # 1120692) and 3 paratypes ( USNM # 1120693, 1120694, 1120695 (egg)) are deposited in the National Museum of Natural History, Smithsonian Institution, Washington, DC, USA . The remaining paratypes are deposited in the GSMNP collection; the Nelson collection in the Department of Biological Sciences, East Tennessee State University, Johnson City, Tennessee, USA ; and the Binda and Pilato collection in the Department of Animal Biology, University of Catania, Catania, Italy . FIGURE 5. Macrobiotus halei sp. nov. A, egg. B, reticular design on the egg processes. C, ornamentation of the egg areolae. (Scale bars = 10.0 µm) Differential Diagnosis: Macrobiotus halei sp. nov. is similar to M. vanescens Pilato, Binda & Catanzaro, 1991 , M. priviterae Binda, Pilato, Moncada & Napolitano, 2001 , M. danielisae Pilato, Binda & Lisi, 2006 , M. lorenae Biserov, 1996 , M. gerlachae Pilato, Binda & Lisi, 2004 , M. corgatensis Pilato, Binda & Lisi, 2002 , M. sklodowskae Michalczyk, Kaczmarek & Weglarska, 2006 , M . alekseevi Tumanov, 2005 , M. garynhai Kaczmarek, Michalczyk & Diduszko, 2005 , and M. magdalenae Michalczyk & Kaczmarek, 2006 in having sculptured egg areolae, but it differs from them in some other characters. M. halei sp. nov. differs from M. vanescens by having small cuticular tubercles (difficult to see) instead of a very fine punctation; stylet supports inserted on the buccal tube in a more caudal position ( pt = 81.0– 82.7 in M . halei sp. nov. , 76.5–77.5 in M. vanescens ); a longer microplacoid ( pt = about 6.0 in M. halei sp. nov. , about 4.0 in M. vanescens ) ( Figs. 4 C and 6A); and a different shape of the egg processes, which are slightly shorter (11.0–14.0 µm in M. halei , 16.0–17.0 µm in M. vanescens ) while the basal diameter is similar (22.0–23.5 µm in M. halei sp. nov. , 24.0–25.0 µm in M. vanescens ). M. halei sp. nov. differs from M. priviterae by lacking eyes and by having a sculptured cuticle, a different shape of the claws ( Figs. 4 C and 6B), egg processes with similar height but a larger basal diameter (22.0–23.5 µm in M. halei sp. nov. , 13.0–16.3 µm in M. priviterae ) and a more evident reticular design with more elongated meshes. M. halei sp. nov. differs from M. danielisae by having different cuticular ornamentation (small circular or elongate tubercles in M. halei sp. nov. , small polygons of variable and irregular shape in M. danielisae ), by having shorter egg processes (11.0–14 µm in M. halei sp. nov. , 17.2 µm in M. danielisae ), of different shape (truncated cones in M. halei sp. nov. , cones in M. danielisae ( Figs. 5 and 6 C), and with the height clearly shorter than the basal diameter while in M. danielisae the height is similar to the basal diameter (the basal diameter is 22.0–23.5 µm in M. halei sp. nov. , 17.5–20.0 µm in M. danielisae ). M. halei sp. nov. differs from M. lorenae by having very small cuticular tubercles and by having a different shape of the egg processes ( Figs. 5 and 6 D): truncated cones in M. halei sp. nov. , and cones terminating with a long, thin, flexible filament in M. lorenae . M. halei sp. nov. differs from M. gerlachae by having a cuticle ornamented with tubercles, slightly shorter and stouter claws ( Figs. 4 D, E and 7A), and larger eggs, with processes having a larger basal diameter (22.0 – 23.5 µm in M. halei sp. nov. , 11.8–14.5 µm in M. gerlachae ) ( Figs. 5 and 7 B). FIGURE 6. A, buccal-pharyngeal apparatus of Macrobiotus vanescens . B, claws of Macrobiotus priviterae . C, egg of Macrobiotus danielisae . D, egg of Macrobiotus lorenae . (Scale bars = 10.0 µm) M. halei sp. nov. differs from M. corgatensis by lacking eyes, and by having small cuticular tubercles instead of a very fine punctation, stouter claws ( Figs. 4 D, E and 7C), and truncated, conical egg processes ( Figs. 5 and 7 D) that are shorter (11.0–14.0 µm in M. halei sp. nov. , 20.0–25.0 µm in M. corgatensis ). M. halei sp. nov. differs from M. sklodowskae by lacking eyes, having a sculptured cuticle, a longer and wider buccal tube (the buccal tube width is 14.2–19.1 µm ( pt = 26.5–28.6) in M. halei sp. nov ., 7.1–11.9 µm ( pt = 17.0–20.5) in M. sklodowskae ), more slender claws, and less slender egg processes with their basal diameter larger with respect to the process height (height 11.0–14.0 µm and basal diameter 22.0–23.5 µm in M. halei sp. nov. ; height 15.2–19.0 µm and basal diameter 29.5 µm in M. sklodowskae ). M. halei sp. nov. differs from M. alekseevi by having a sculptured cuticle, longer stylet supports inserted on the buccal tube in a more caudal position ( pt = 81.0– 82.7 in M. halei sp. nov. , 78.1–81.0 in M. alekseevi ), a wider buccal tube ( pt = 26.5–28.6 in M. halei sp. nov. , 16.6–20.3 in M. alekseevi ) and differently-shaped egg processes (in M. halei sp. nov. they are truncated cones without a terminal cap-like vesicular structure). FIGURE 7. A, claws on the second and third pairs of legs of Macrobiotus gerlachae . B, egg of Macrobiotus gerlachae . C, claws on the first and second pairs of legs of Macrobiotus corgatensis . D, egg of Macrobiotus corgatensis . (Scale bars = 10.0 µm) M. halei sp. nov. differs from M. garynhai by having cuticular tubercles and lacking elliptical pores, and by having egg processes that are truncated cones without a cap-like terminal portion, and by the egg shell areolae with a thickened central portion with circular light spots forming an irregular reticular design). M. halei sp. nov. differs from M. magdalenae by lacking eyes, having a sculptured cuticle, and by the shape of the egg processes (truncated cones in M. halei sp. nov. , cones with elongate terminal portion in M. magdalenae ). Comments: Besides the holotype and paratypes used for the description of the species in this paper, additional specimens of Macrobiotus halei sp. nov. have been identified within the park. The total is now 361 specimens , including 13 eggs , although we expect to find many more as we sort through additional slides of the M. richtersi group. So far, all M. halei sp. nov. specimens have been found in mosses and lichens on trees and rocks and also frequently in soil and leaf litter. None have been found in our aquatic samples.