Supplementary Materials and Appendix
Author
Zhang, Jing
McDermott Center for Human Growth and Development and Department of Biophysics University of Texas Southwestern Medical Center 5323 Harry Hines Blvd., Dallas, TX, 75390 - 8816 USA
Author
Cong, Qian
McDermott Center for Human Growth and Development and Department of Biophysics University of Texas Southwestern Medical Center 5323 Harry Hines Blvd., Dallas, TX, 75390 - 8816 USA
Author
Grishin, Nick V.
Departments of Biophysics and Biochemistry University of Texas Southwestern Medical Center 5323 Harry Hines Blvd., Dallas, TX, 75390 - 9050 USA
text
Insecta Mundi
2023
2023-12-29
2023
26
1
115
http://dx.doi.org/10.5281/zenodo.10396362
journal article
10.5281/zenodo.10396362
1942-1354
Myrinia manchada
Grishin
,
new species
https://zoobank.org/
F341D06C-4741-4C54-ABCE-A7BD83C5C264
(
Fig. 2
part, 67–68, 282–284)
Definition and diagnosis.
Sequencing of the
holotype
of
Myrinia laddeyi
(E. Bell, 1942)
(type locality in
Ecuador
) revealed that it is a species related to
Myrinia binoculus
(Möschler, 1877)
(type locality in
Suriname
) and not the species
Evans (1953)
identified as
M. laddeyi
(
Fig. 2
). Therefore, the species that Evans misidentified as
M. laddeyi
does not have an available name and is new. In USNM, we found a specimen that keys to E.
24.4 in
Evans (1953)
and is distinguished from its relatives, including the true
M. laddeyi
, by larger size (forewing typically longer than
21 mm
), more segments in nudum (more than 25 segments), the lack of eyespot on the ventral forewing, large dark tornal spot on the ventral hindwing, short and rounded harpe of the right valva, style of the left valva longer than harpe. This species is not cryptic and is diagnosed reliably by phenotype. In DNA, a combination of the following base pairs is diagnostic in the nuclear genome: aly798.33.50:A117G, aly
2149.2.8
:G45A, aly38.5.1:T141C, aly767.7.2:T78C, aly767.7.2:T468A, aly651.4.5:T106T (not C), aly536.173.2:C78C (not T), aly17.12.4:C112C (not T), aly17.12.4:A336A (not G), aly
1022.2.1
:G96G (not A), and COI barcode: T82C, 277C, T364A, T352C, T386C.
Barcode sequence of the
holotype
.
Sample 11-BOA-13383A10, GenBank OR837652, 658 base pairs: AACTTTATATTTTATTTTTGGAATTTGAGCAGGAATAGTTGGAACATCTTTAAGTTTATTAATTCGAACTGAATTAGGTAACCCAGGATCATTAATT GGAGATGATCAAATTTATAATACTATTGTTACAGCTCATGCTTTTATTATAATTTTTTTTATAGTTATACCAATTATAATTGGAGGATTTGGAAATT GACTTGTACCATTAATATTAGGAGCCCCAGATATAGCTTTCCCCCGAATAAATAATATAAGATTTTGATTATTACCCCCCTCCTTAATATTATTAAT CTCCAGAAGAATTGTAGAAAATGGAGCAGGAACTGGATGAACTGTTTATCCCCCTTTATCCGCTAATATTGCACACCAAGGATCTTCAGTAGACCTA GCTATTTTTTCTCTACATTTAGCTGGAATTTCATCTATTTTAGGAGCTATCAATTTTATTACAACAATTATTAATATACGTATTAATAATCTTTCAT TTGATCAAATACCATTATTCGTTTGAGCTGTAGGAATCACAGCTTTATTATTATTATTATCTTTACCTGTATTAGCAGGTGCTATTACTATACTTCT AACAGATCGAAATTTAAATACATCTTTTTTTGATCCTGCTGGAGGAGGAGATCCTATTCTTTATCAACATTTATTT
Type material.
Holotype
:
♂
deposited in the
National Museum of Natural History
,
Smithsonian Institution
,
Washington
,
DC
, USA (
USNM
), illustrated in
Fig. 67–68
, bears the following five rectangular labels, four white: [
GUYANA
: Region 8 | Potaro River nr. Tukeit | 250′ – 1000′ |
18-23 Mar 1999
| leg.
S. Fratello
], [DNA sample ID: | 11-BOA-13383A10 | c/o
Nick
V
.
Grishin
], [DNA sample ID: | NVG-22034H05 | c/o
Nick
V
.
Grishin
], [{
QR Code
}|
USNM
ENT 00232398
], and one red [
HOLOTYPE
♂
|
Myrinia
|
manchada Grishin
]
.
Paratype
:
1♂
: NVG-23026F10, EL83681 French Guiana, Saint-Laurent-du-Maroni, 1905, E. Le Moult leg. [
MNHP
].
Type
locality.
Guyana
:
Potaro-Siparuni Region
, Potaro River nr. Tukeit.
Etymology.
In Spanish,
manchada
means stained, spotted, spotty, or smudgy. The name is given for the large tornal spot on the ventral hindwing and is a noun in apposition.
Distribution.
The Guianas.