DNA barcodes reveal 63 overlooked species of Canadian beetles (Insecta, Coleoptera) Author Pentinsaari, Mikko Author Anderson, Robert Author Borowiec, Lech Author Bouchard, Patrice Author Brunke, Adam Author Douglas, Hume Author Smith, Andrew B. T. Author Hebert, Paul D. N. text ZooKeys 2019 894 53 150 http://dx.doi.org/10.3897/zookeys.894.37862 journal article http://dx.doi.org/10.3897/zookeys.894.37862 1313-2970-894-53 D11503CA5A574067817904E0C8C162C8 BAF8B2CC491254A3AC7E08368A2697B5 Chilocorus renipustulatus (Scriba, 1791) Figure 33 Distribution. Native to the Palaearctic region. Widespread in Europe, also recorded from Siberia and the Russian Far East ( Kovar 2007 ). Adventive in the Nearctic region (Ontario, Canada). Canadian records. Ontario: Hamilton, 22-Sep-2014 to 03-Oct-2014 (1 ex, CBG); Mississauga, 19-Sep-2016 to 30-Sep-2016 (3 exx, CBG); Windsor, 22-Sep-2014 to 03-Oct-2014 (1 ex, CBG). Diagnostic information (based on Fuersch 1967 and Gordon 1985 ). Body length 4-5 mm. Habitus as in Fig. 33A . Black, shiny, with a single rounded or slightly transverse orange-red macula on each elytron, abdomen laterally and apically orange, medial part of first ventrite black. Pronotum without distinct microsculpture on disc. Male genitalia as in Fig. 33B, C . Bionomic notes. The main habitat in Europe is broadleaf forest, and the preferred prey are scale insects, in particular Chionaspis salicis (Linnaeus, 1758) ( Koch 1989b ). The Canadian specimens were collected with Malaise traps in suburban residential areas. Comments. Chilocorus kuwanae Silvestri, 1909, an East Asian species introduced to the United States and recorded from across the country ( Gordon 1985 ; Hendrickson et al. 1991 ), was recently synonymized with C. renipustulatus by Bienkowski (2018) . According to Bienkowski , male genitalia are similar throughout the distribution areas of both species. However, Bienkowski did not study any type material. One of the Canadian specimens shares an identical barcode haplotype with specimens of C. renipustulatus from Germany and Finland, others are slightly divergent (p-distance to European material varies from 0.006 to 0.015). Unfortunately, no barcode data are available for C. kuwanae . No Canadian records have been previously published under either name. Chilocorus renipustulatus is externally very similar to Chilocorus stigma (Say, 1835) and its closest relatives. It can be distinguished using the male genitalia and microsculpture of the pronotum. In C. stigma and allied species, the interspace between punctures on the disc of the pronotum is covered by finely engraved, netlike microsculpture. In C. renipustulatus , the interspace is smooth and shiny, with no visible microsculpture on disc. The orange maculae on the elytra are more transverse in C. renipustulatus than in C. stigma in the examined DNA barcoded Canadian material of these species, but the maculae are known to vary in size and shape in C. renipustulatus ( Bienkowski , 2018). Figures 33, 34. 33 Chilocorus renipustulatus (Scriba) 33A habitus, L. Borowiec 33B median lobe (penis guide) and parameres, ventral view 33C penis (sipho), tip in lateral view 34 Nephus bisignatus (Boheman) 34A habitus, S. Karjalainen 34B median lobe (penis guide) and parameres, lateral view 34C penis (sipho), tip in lateral view. Scale bars: 1.0 mm ( 33A ), 0.5 mm ( 33B; 34A ), 0.2 mm ( 33C; 34B ), 0.1 mm ( 34C ).