The xerophilic genera Xerobiotus and Pseudohexapodibius (Macrobiotidae; Tardigrada): biodiversity, biogeography and phylogeny Author Vincenzi, Joel Department of Life Sciences, University of Modena and Reggio Emilia, Modena, Italy joel.vincenzi@unimore.it Author Cesari, Michele Department of Life Sciences, University of Modena and Reggio Emilia, Modena, Italy & NBFC, National Biodiversity Future Center, Palermo, Italy Author Kaczmarek, Łukasz Department of Animal Taxonomy and Ecology, Faculty of Biology, Adam Mickiewicz University, Poznań, Poland Author Roszkowska, Milena Department of Animal Taxonomy and Ecology, Faculty of Biology, Adam Mickiewicz University, Poznań, Poland Author Mioduchowska, Monika Department of Evolutionary Genetics and Biosystematics, Faculty of Biology, University of Gdansk, Poland Author Rebecchi, Lorena Department of Life Sciences, University of Modena and Reggio Emilia, Modena, Italy & NBFC, National Biodiversity Future Center, Palermo, Italy Author Guideưi, Yevgen Kiosya and Roberto text Zoological Journal of the Linnean Society 2024 2023-10-10 200 1 111 141 http://dx.doi.org/10.1093/zoolinnean/zlad129 journal article 10.1093/zoolinnean/zlad129 0024-4082 10470587 Xerobiotus reductus sp.nov. ( Figs 6 , 7 ; measurements and statistics are in Tables 2 and 3 ; Supporting Information, Table S1 ). ZooBank: urn:lsid:zoobank.org:act: 0116DF21-ACDA-436F-806C-A4E1DE09E979 Type locality: Burgenland , near Rust , Austria ( 47°47ʹ59″N , 16°40ʹ42″E , 118 m a.s.l. ), moss from sandy soil, September 2019 , coll. Johenn Sholl. Additional locality: Notecka Forest , Wiełkopolska Province ( Poland ) ( 52°48ʹ48.32″N , 16°14ʹ59.49″E ), in a clump of grass . Material examined: Eighty-four adults and 28 eggs (slides AU1–AU8). Type repositories: One hundred and six specimens deposited at the Department of Animal Taxonomy and Ecology , Institute of Environmental Biology , Adam Mickiewicz University in Poznań , Poznań , Poland ; five specimens [slides: M8/17 and M8/27 ( four adults and one egg )] deposited at the Institute of Systematics and Evolution of Animals , Polish Academy of Sciences , Kraków , Poland . Etymology: The name of the species refers to the reduced claws on all pairs of legs. Description: Body whitish, eye-spots present (in ≥ 60% of fixed and measured specimens). Very small, scaưered pores (~0.5 µm in diameter; visible only with SEM; Fig. 7C, E, F ) in the dorsolateral cuticle of the body and legs. Very small single granules (visible only with SEM), distributed almost regularly, present on the entire cuticle ( Fig. 7E ). Legs of the first pair clearly smaller than those of the second and third pairs. The area of the leg cuticle surrounding the claws with a swelling (forming a furbelow-like structure; Figs 6A , 7C, F ) covered with spheroidal microdigitations visible with SEM ( Fig. 7D ). Buccopharyngeal apparatus of the Macrobiotus type , with anteroventral mouth and 10 small peribuccal lamellae ( Fig. 7A, B ). Oral cavity armature of the maculatus type (with LM), with only third band of teeth (transversal crests) formed by one continuous dorsal tooth (ridge) and ventral transversal ridges as two-to-three granular teeth ( Fig. 6 D-F); with SEM, first (with one or two rows of teeth) and second (with two or three rows of a bit larger teeth) bands visible ( Fig. 7B ). Typically shaped stylet furcae, with oval condyles supported by short branches provided with rounded apophyses. In the pharynx ( Fig. 6C ): large and triangular pharyngeal apophyses overlapping the first macroplacoid; two rod-shaped macroplacoids (in lateral view), length sequence 2 <1, and evident triangular microplacoid. In frontal view, the first macroplacoid in the shape of a drop with a median slight constriction, the second rectangular with rounded corners and with a subterminal slight constriction, at least in some specimens. Claws I–III of Xerobiotus type , small and compact, with the common tract similar in length to the main branch; main branch with accessory points; lunules absent ( Figs 6G, H , 7C ). Claw IV with a longer common tract, smaller and shorter claw branches, without lunules or thickening under the claws ( Figs 6I, J , 7F ). Primary branches of all claws with small accessory points. Eggs spherical, white, ornamented and laid freely ( Figs 6K , 7G ). Surface between processes of hufelandi type , i.e. covered with a reticulum formed by a mesh of small, densely distributed pores, uniform in size and evenly distributed ( Figs 6L, M , 7H ). Processes of the hufelandi type , with a straight trunk and a relatively small and concave terminal disc. The terminal disc greatly indented on the disc margin, forming evident but irregular teeth covered by microgranules (visible only with SEM; Fig. 7H, I ). Reproduction: Gonochoristic amphimictic species; females and male with spermatozoa present ( Fig. 6B ). The diploid karyotype resulted in six chromosomes (2 n ) ( Fig. 6N ). Molecular characterization: One haplotype for cox1 , two haplotypes for ITS2 (p-distance 0.5%), one haplotype for 18S, and one haplotype for 28S genes (GenBank accession numbers in Supporting Information, Table S1 ; p-distances in Supporting Information,Table S8). The more similar sequences of X.reductus belong: for cox1 , to X. naginae and to specimens from Skwierzyna population with p-distances of 1.3% and 1.9%, respectively; for ITS2, to X. naginae and P. degenerans with p-distances of 0.0%- 0.3% and 0.0%-0.8%, respectively (Supporting Information, Table S8). Differential diagnosis: Xerobiotus reductus differs from: X. euxinus by the absence of semilunar thickening at the base of the claws I–III and absence of lunules in claw IV; X. gretae by shorter branches of claws I–III and absence of lunules and cuticular thickening at the base of claw IV; X. naginae by the third band of teeth (transversal crests) visible with LM and formed by one continuous dorsal tooth (ridge); X. xerophilus by the absence (visible with SEM) of cuticular plates at the base of all claws (see Dastych and Alberti 1990 ) and the shape of egg processes (without a funnel-like depression).