Systematic revision of the parasitoid wasp genus Glyptapanteles Ashmead (Hymenoptera: Braconidae: Microgastrinae) for Australia results in a ten-fold increase in species Author Fagan-Jeffries, Erinn P. C724E269-029E-49E8-8D95-6F5A5DA6BAAF Australian Centre for Evolutionary Biology & Biodiversity and School of Biological Sciences, The University of Adelaide, Australia. & South Australian Museum, Adelaide, Australia. erinn.fagan-jeffries@adelaide.edu.au Author McCLELLAND, Alana R. 3FDC78D1-CDF3-472F-B4EE-63A43C1730AF Australian Centre for Evolutionary Biology & Biodiversity and School of Biological Sciences, The University of Adelaide, Australia. alana.mcclelland@adelaide.edu.au Author Bird, Andrew J. DC97FEB2-1BB0-48CE-9178-0C5F98131CC0 Australian Centre for Evolutionary Biology & Biodiversity and School of Biological Sciences, The University of Adelaide, Australia. andrewbird@ozemail.com.au Author Giannotta, Madalene M. FF66BA72-4585-402F-AA42-61C9B7856048 Australian National Insect Collection, CSIRO, Black Mountain, ACT, Australia and Centre for Biodiversity Analysis, Research School of Biology, Australian National University, Acton, ACT, Australia. madalene.giannotta@gmail.com Author Bradford, Tessa M. D018F430-ED59-47BA-BF6A-EF8C6675AC20 Australian Centre for Evolutionary Biology & Biodiversity and School of Biological Sciences, The University of Adelaide, Australia. & South Australian Museum, Adelaide, Australia. tessa.bradford@samuseum.sa.gov.au Author Austin, Andrew D. DE71F924-750D-490D-84A7-F5960066F7CC Australian Centre for Evolutionary Biology & Biodiversity and School of Biological Sciences, The University of Adelaide, Australia. & South Australian Museum, Adelaide, Australia. andrew.austin@adelaide.edu.au text European Journal of Taxonomy 2022 2022-02-08 792 1 1 116 http://dx.doi.org/10.5852/ejt.2022.792.1647 journal article 20694 10.5852/ejt.2022.792.1647 0d881922-a259-4986-99d8-8fc3919204b0 2118-9773 6037052 18DB5F54-5CEB-498E-A6F1-E570E6A57833 Glyptapanteles rodriguezae Fagan-Jeffries, Bird & Austin sp. nov. urn:lsid:zoobank.org:act: 04BFEC74-5C1A-40F1-B10D-FAD918E18883 Fig. 49 Diagnosis Glyptapanteles rodriguezae sp. nov. is in the G. arcanus species group and can be separated from the other members of the species group, other than from Glyptapanteles ruhri sp. nov. , by the hind femur being pale in colouration and propodeum punctate with smooth areas between punctures and with littleto-no rugosity and T1 punctured in the posterior half. There was no external morphological character found which easily separates G. rodriguezae sp. nov. from G. ruhri sp. nov. and we do not provide a morphological diagnosis between these two species. The two species are sister lineages in the current phylogeny ( Fig. 2 ), but Glyptapanteles ruhri sp. nov. is 6 bp different in the wingless sequence from G. rodriguezae sp. nov. and>6% divergent in the COI barcode and we therefore feel confident that they are different species. Etymology This species is named for Dr Juanita Rodriguez, who was part of the team who collected the type specimen and who has an endlessly enthusiastic and collaborative approach to hymenopteran research. Material examined Holotype AUSTRALIA • ♀ ; Australian Capital Territory , CSIRO Black Mountain ; -35.2744 , 149.1115 ; 6 Dec. 2017 – 5 Jan. 2018 ; J. Rodriguez , C. Waichert , K.M. Bayless and T. Pleines leg.; Malaise 2 , green dry wash ; Extraction979, BOLD: AUGLY031-21; ANIC 32 130332 . Fig. 49. Glyptapanteles rodriguezae Fagan-Jeffries, Bird & Austin sp. nov. , A, C, E . Paratype, ♀ (ANIC 32 130333). B, D, F–G . Holotype, ♀ (ANIC 32 130332). A . Lateral habitus. B . Fore wing. C . Dorsal habitus. D . Lateral head. E . Dorsal metasoma. F . Dorsal head. G . Anterior head. Paratype AUSTRALIA • 1 ♀ ; Australian Capital Territory , CSIRO Black Mountain ; -35.268 , 149.1066 ; 650 m a.s.l. ; 11–25 Feb. 2020 ; K.M. Bayless leg.; Y-shaped wash, Malaise trap collapsed by flood ; Extraction1138, BOLD: AUGLY062-21; ANIC 32 130333 . Description Female COLOURATION. Gena without a pale spot; labrum reddish-brown; scape colour in ventral half uniformly paler than flagellomeres; flagellomeres all black/dark brown or darkening distally; tegula pale; wing veins uniformly black or brown, or with small lighter area proximally; anteromesoscutum all dark; scutellar disk and metanotum dark; propodeum dark; fore coxa pale yellow; mid coxa pale yellow; hind coxa dark; fore femur pale yellow; mid femur pale yellow; hind femur pale yellow; fore tibia pale yellow; mid tibia pale yellow; hind tibia darkening posteriorly; hind basitarsus light brown; T1 dark; T2 sclerotised area dark; T2 lateral area much paler; T3 mostly pale with darker patch in centre or mostly dark with paler lateral areas; T4–5 pale with dark patch in centre, then darkening posteriorly on T6–7. HOLOTYPE BODY MEASUREMENTS. Body length 2.4 mm ; fore wing length 2.4 mm ; antennal length slightly longer than body length. HEAD. Antennal flagellomere 14 length/width 1.83–2.16; antennal flagellomere 2 length/width 2.87– 3.00; OOD/POD 1.75–2.17; IOD/POD 1.38–2.00. MESOSOMA. Anteromesoscutum sculpturing with shallow to deep punctures, space between punctures a mixture of smaller than diameter of punctures and of similar size, often smoother in posterior centre; scutellar disk sculpturing with shallow punctures scattered over most of area; 10–11 pits in scutellar sulcus; propodeum with median carina absent, propodeum punctate. WINGS. Pterostigma length 0.63 mm ; pterostigma width 0.22 mm ; r 0.23 mm ; 2RS 0.17 mm ; 2m 0.12 mm ; (RS+M)b 0.07 mm . METASOMA. T1 lateral edges parallel for anterior ½ to ⅔ of length, then narrowing posteriorly; T1 mostly smooth, sometimes shiny, sometimes with punctures in posterior half and/or on lateral edges; T1 length 0.42 mm ; T1 width at posterior edge 0.12 mm ; T2 an isosceles trapezoid, lateral edges straight; T2 smooth and shiny or with some shallow indistinct sculpturing; T2 length 0.15 mm ; T2 width at posterior edge 0.26 mm ; ovipositor slightly protruding from end of metasoma. Male Unknown. Remarks Glyptapanteles rodriguezae sp. nov. constitutes BIN BOLD:AAU5026 and is 6.25% (p-dist.) divergent from the closet BIN in the database (BOLD:ADL3583; Glyptapanteles ruhri sp. nov. ). Using the BOLD Batch ID engine, the DNA barcode of the holotype is 6.3% different from the most similar sequence from an Australian specimen (AUMIC418-18; Glyptapanteles ruhri sp. nov. ). Both type specimens were sequenced for the wingless gene, which is identical between them and differs by a minimum of 6 bp from all other species with available sequence data. There is another specimen in the BOLD database, ASQAS187-11, which has an identical COI sequence, but the location data is listed only as Australia and there are no images available. Distribution This species is known from the ACT.