Alloperla clarki sp. nov. (Plecoptera: Chloroperlidae), a new species from the eastern Nearctic with discussion of a new species group Author Grubbs, Scott A. Author Baumann, Richard W. text Zootaxa 2019 2019-06-28 4624 2 241 255 journal article 26359 10.11646/zootaxa.4624.2.7 83eecd69-03b2-4cc5-beaf-e3682312ffb4 1175-5326 3259313 90BF05DF-3EC4-4E77-A1DC-EBDF6EC4C83A Alloperla biserrata Nelson & Kondratieff, 1980 Dusky Sallfly http://lsid.speciesfile.org/urn:lsid: Plecoptera .speciesfile.org:TaxonName:3649 ( Figs. 1–12 & 39 ) Alloperla biserrata Nelson & Kondratieff 1980:802 . Holotype male (USNM), spring-fed stream flowing into Craigs Creek , Montgomery Co. , Virginia (examined) Alloperla biserrata : Surdick 2004:12 Alloperla biserrata : Willett & Stark 2009:121 Distribution. USA : MD, PA, VA, WV ( DeWalt et al. 2018 ) Material examined. USA , Maryland , Allegany Co. , Biclic Run , Green Ridge State Forest , 39.66440 , -78.46144 , 13 May 1996 , S.A. Grubbs , 1 male , 1 female ( WKUC ) ; Deep Run , Green Ridge State Forest , 39.65312 , -78.45234 , 13 May 1996 , S.A. Grubbs , 1 male ( WKUC ) ; spring into Fifteenmile Creek , Green Ridge State Forest , 39.67138 , -78.45970 , 13 May 1996 , S.A. Grubbs , 1 male ( WKUC ) ; tributary to Fifteenmile Creek , Cow Hollow , Green Ridge State Forest , 39.69968 , -78.47884 , 13 May 1996 , S.A. Grubbs , 1 male ( WKUC ) ; tributary to Fifteenmile Creek , Little Pine Lick Hollow , Green Ridge State Forest , 39.68344 , -78.47307 , 13 May 1996 , S.A. Grubbs , 1 male ( WKUC ) ; tributary to Fifteenmile Creek , Green Ridge State Forest , 39.68658 , -78.47720 , 13 May 1996 , S.A. Grubbs , 1 male , 1 female ( WKUC ) ; tributary to Fifteenmile Creek , Cow Hollow , Green Ridge State Forest , 39.69910 , -78.47831 , 13 May 1996 , S.A. Grubbs , 3 males , 2 females ( WKUC ) ; tributary to Fifteenmile Creek , White Oak Hollow , Green Ridge State Forest , 39.70665 , -78.48404 , 13 May 1996 , S.A. Grubbs , 1 male , 1 female ( WKUC ) ; tributary to Black Sulphur Run , Green Ridge State Forest , 39.66716 , -78.52963 , 18 May 1996 , S.A. Grubbs , 7 males , 4 females ( WKUC ) ; Black Sulphur Run , Green Ridge State Forest , 39.66614 , -78.49731 , 18 May 1996 , S.A. Grubbs , 5 males , 2 females ( WKUC ) ; spring into Big Run , Green Ridge State Forest , 39.57145 , -78.51005 , 18 May 1996 , S.A. Grubbs , 3 males , 2 females ( WKUC ) ; Big Run , Rte. 51, Green Ridge State Forest , 39.52642 , -78.53212 , 18 May 1996 , S.A. Grubbs , 17 males , 6 females ( WKUC ) ; Big Run , Green Ridge State Forest , 39.57491 , -78.50910 , 18 May 1996 , S.A. Grubbs , 12 males , 6 females ( WKUC ) ; Deep Run , Green Ridge State Forest , 39.65312 , -78.45234 , 4 July 1996 , S.A. Grubbs , 2 females ( WKUC ) . Pennsylvania , Bedford Co. , tributary to Bear Camp Branch , 11 km SSE Chaneysville , Buchanan State Forest , 39.72623 , -78.45226 , 14 May 1999 , S.A. Grubbs , 1 male , 1 female ( WKUC ) . Virginia , Augusta Co. , Calf Pasture River , 10 June 1997 , M.F. Whiting , 1 male ( BYU ) ; Montgomery Co. , Craigs Creek , Route 621, 0.5 miles off Route 460, 29 May 1979 , B.C. Kondratieff , 2 males ( CHNC ) ; same but 27 June 1979 , B.C. Kondratieff , 1 male ( RFKC ) ; same but 5 June 1983 , B.C. Kondratieff , 3 males , 2 females ( BYU ) ; Page Co. , Jeremy Run , east of Vaughn , 8 May 2001 , M. Terry , 1 male ( BYU ) . FIGURES 1–8 . Alloperla biserrata , adult male epiproct. 1, Maryland, Big Run, dorsal view, 300X; 2, Maryland, Black Sulphur Run, dorsal view, 1300X; 3, Maryland, Black Sulphur Run, dorsal view, 2500X; 4, Maryland, Black Sulphur Run, caudal view, 2500X; 5, Virginia, Craig Creek, lateral view, 3500X; 6, Virginia, Craig Creek, anterior view, 4500X; 7, Maryland, Black Sulphur Run, dorsal view, 5000X; 8, Virginia, Craig Creek, dorsal view, 2500X. Amended description – Male. Tergum 10 divided mesally by epiproct and reduced cowl into two hemitergal lobes; lobes bearing dense matting of long setae ( Figs. 1–2 ). Epiproct length (L) 240 µm , maximum width ( MW ) 47 µm , L:MW ratio = 5.1. In dorsal view the epiproct is widest in basal ⅓, tapering anteriorly to a slightly mediallyexpanded apex ( Figs. 1–2 ); dorsal surface covered by dense matting of long hairs in medial ⅓; apical ⅓ is entirely bare; in basal ⅓ there is only a medial line of hairs ( Figs. 1–4 ). The apical notch is narrowly triangular in shape and extends anteriorly and ventrally as a groove between paired, serrated apical margins; 5–6 distinct teeth are present ( Figs. 5–8 , 10, 12 ). In lateral view the epiproct is thin basally and gradually broadens apically ( Figs. 9, 11 ); matting is absent basally and along the ventral margins; long hairs deflected laterally are present in medial ½ ( Figs. 9, 11 ); shorter hairs on the apical, serrated portion are present as a medial band ( Figs. 10, 12 ). The stem length is approximately 2X the length of the apical serrated portion ( Figs. 9, 11 ). Overall, the SEM of the specimens studied herein from Maryland and Virginia are essentially identical in shape and matting patterns to the Virginia population studied in Willett & Stark (2009 , their figs. 55–60). FIGURES 9–12 . Alloperla biserrata , adult male epiproct. 9, Maryland, Black Sulphur Run, lateral view, 1500X; 10, Maryland, Black Sulphur Run, lateral view, 3500X; 11, Virginia, Craig Creek, lateral view, 1500X; 12, Virginia, Craig Creek, lateral view, 3500X. Adult males (and females) are pale yellow in alcohol. The head lacks any darkened markings. The pronotum has a continuous pale yellow margin and the medial 75% of the segment is only slightly darker. Comments. Reexamination of a specimen designated as a paratype produced an unexpected result. The male collected from “…confluence of Straley Branch with Poverty Creek off County Route 708, Montgomery Co., Virginia , Jefferson National Forest, altitude 630 m , 17 June 1978 ” ( Nelson & Kondratieff 1980 , see pg. 802) is actually of A. stipitata . Alloperla biserrata occupies a relatively narrow latitudinal and longitudinal band in the southern and central Appalachian Mountains ( Fig. 39 ). Amongst the four members of the Alloperla nanina Group, this species is the only one known northward to Maryland and southern Pennsylvania .