Description of three new species of biting midge of the genus Atrichopogon Kieffer (Diptera: Ceratopogonidae) from Brazilian Amazon Author Farias, Emanuelle De Sousa 0000-0001-5949-877X Instituto Leônidas e Maria Deane - FIOCRUZ. Laboratório de Ecologia e Doenças Transmissíveis na Amazônia, Manaus, Amazonas, Brazil. & Instituto Oswaldo Cruz-IOC / FIOCRUZ. Programa de Pós-Graduação em Biodiversidade e Saúde, Rio de Janeiro, Rio de Janeiro, Brazil. & emanuellefarias 82 @ gmail. com; https: // orcid. org / 0000 - 0001 - 5949 - 877 X emanuellefarias82@gmail.com Author Santos, Sanmya Silva Dos 0000-0001-7377-8735 Instituto Leônidas e Maria Deane - FIOCRUZ. Laboratório de Ecologia e Doenças Transmissíveis na Amazônia, Manaus, Amazonas, Brazil. & Instituto Leônidas e Maria Deane - FIOCRUZ. Programa de Bolsa de Iniciação Científica - PIBIC / CNPq. & sanmyasantos 23 @ gmail. com; https: // orcid. org / 0000 - 0001 - 7377 - 8735 sanmyasantos23@gmail.com Author Paulino-Rosa, Jokebede Melynda Dos Santos 0000-0003-2359-4836 Instituto Leônidas e Maria Deane - FIOCRUZ. Laboratório de Ecologia e Doenças Transmissíveis na Amazônia, Manaus, Amazonas, Brazil. & Instituto Leônidas e Maria Deane - FIOCRUZ. Programa de Pós-Graduação em Biologia da Interação Patógeno Hospedeiro, Manaus, Amazonas, Brazil. & jokebedemelynda @ gmail. com; https: // orcid. org / 0000 - 0003 - 2359 - 4836 jokebedemelynda@gmail.com Author Pessoa, Felipe Arley Costa 0000-0002-6318-1887 Instituto Leônidas e Maria Deane - FIOCRUZ. Laboratório de Ecologia e Doenças Transmissíveis na Amazônia, Manaus, Amazonas, Brazil. & facpessoa @ gmail. com; https: // orcid. org / 0000 - 0002 - 6318 - 1887 facpessoa@gmail.com text Zootaxa 2021 2021-04-09 4952 2 275 290 journal article 7436 10.11646/zootaxa.4952.2.4 88cc79b6-ea26-4742-b872-6f5df6544661 1175-5326 4674037 642A07AB-5A2F-4514-9DF2-70E5ACECC140 Atrichopogon riopardensis Farias, Pessoa & Paulino-Rosa sp. nov. ( Figs. 7 A–E ; 8 A–D ; 9 A–F ; 10 A–F ; 11 A–E ) Diagnosis . Male. Only extant species of Atrichopogon with pigmented wings in the Neotropical region with gonostylus forked near its midlength; inner portion short, like a fingernail, with longitudinal furrow; outer portion, apically curved directed mesally, 4 × longer than inner, with one long seta in basal 1/3. Female. Without good characteristic features: wing with two distinct darker spots; uniform color pattern of the antenna and legs; one short, ovoid, sclerotized spermatheca with short and broad neck. Male ( Fig. 7C ). Head ( Fig. 7A ); head width/mouthpart length 2.60–3.00 (2.80, n = 7). Ommatidia with interfacet pubescence; broadly abutting medially for length of 3.9–4.4 (4.16, n = 5) ommatidia ( Fig. 7D ). Antenna pale brown; proportions of flagellomeres as in Figure 7E ; flagellomeres 2–10 fused; plume on flagellomeres 1–9 well developed; flagellomeres 10–13 without plume; 9th flagellomere 0.83 times shorter than flagellomere 10; flagellomere 13 with apical projection not basally constricted. AR 0.99–1.10 (1.00, n = 5). Palpus pale brown ( Fig. 7B ); third segment short to moderately elongate, swollen at mid-length, with well-developed pit somewhat apical to mid length; segments 4–5 separated; segment 5 conical. PRIII 2.30–2.70 (2.40, n = 7). Thorax dark brown ( Fig. 8B ). Scutum with setae arising directly from surface, with lateral sutures ( Fig. 8D ). Paratergite with one seta. Anepisternum well-developed, broadly bilobed posteriorly. Wing with patch of pigmentation in area of r-m and posterior to apex of R 3 ( Fig. 8A ); macrotrichiae on membrane in apical portion of r 3 , m 1 and in apical half of M 1 ; wing length 0.85–0.95 (0.90, n = 8) mm. CR 0.66–0.74 (0.68, n = 8). Halter stem pale brown, knob hyaline. Legs ( Fig. 8C ) yellowish brown; hind tibial spur shorter than width of hind tibia, hind tibial comb with 9 spines. TRI 3.37 (n = 1), TRII 3.24 (n = 1), and TRIII 2.63 (n = 1); empodia present. Abdomen ( Fig. 9A ). Segments 1–8 yellowish brown, with ventrolateral black marks well developed on segments 1–3, 5 and 6, smaller on segment 4; distal segments 9–10 dark brown. Terminalia brown ( Figs. 9B, C ); segment 9 equal in width to segment 8; tergite 9 moderately elongate, extending to about level of apex of gonocoxite; posterior margin rounded. Sternite 9 with posterior margin nearly straight and scattered row of setae. Gonocoxite without the medial lobe, with three large setae laterally in the proximal inner part ( Fig. 9C ); length 3.00–3.20 (3.10, n = 6) × the basal width. Gonostylus forked near midlength ( Fig. 9F ); inner portion short and fingernail-like, with longitudinal furrow; outer portion elongate and apically curved directed mesally, 4 × longer than inner portion, with one long seta in basal 1/3. Aedeagal-parameral complex quadrangular ( Figs. 9D, E ), with delicate lateral arms curved posteriorly; dorsal portion sclerotized on distal 1/3, bearing two anteroposteriorly directed projections, ending as process laterally directed; ventral portion with apical part pointed. Cercus short, slightly tapering, extending to the apex of tergite 9. FIGURE 6. Atrichopogon janseni sp. nov. Female. A. Wing; B. Thorax, lateral view; C. Fore, mid- and hind legs (left to right), lateral view; D. Abdomen, ventral view; E. Spermatheca. Female ( Fig. 10C ). Similar to male. Head ( Fig. 10B ); head width/mouth part length 2.80 (n = 1). Eyes broadly abutting medially for length 4.20 (n = 1) ommatidia ( Fig. 10D ). Antenna medium brown; flagellomeres 1–8 vasiform ( Fig. 10A ). AR 1.40 (n = 1). Mandible well developed, with 24 teeth (n = 1) ( Fig. 10E ). Palpus medium brown; third segment short ( Fig. 10F ), pit at mid-length; segments 4–5 separate. PRIII 2.25 (n = 1). Thorax dark brown ( Fig. 11B ). Wing ( Fig. 11A ) pattern of pigmented membrane as observed in males; macrotrichiae on membrane in apical portion of r 3 , m 1 , and apical half of M 1 ; wing length 0.90 (n = 1) mm. CR 0.77 (n = 1). Legs as shown in figure 11E. Abdomen yellowish brown ( Fig. 11D ), with ventrolateral black marks well developed on segments 1–3, 5 and 6, smaller on segment 4; segments 8–10 pale brown; cercus pale brown. Genitalia. One dark brown ovoid spermatheca ( Fig. 11C ), measuring 72.5 × 70.0 (n = 1) μm, with a short neck, 7.5 (n = 1) μm. FIGURE 7. Atrichopogon riopardensis sp. nov. Male. A. Head, anterior view; B. Maxillary palpus; C. Adult, lateral view; D. Eyes separation, anterior view; E. Antenna. FIGURE 8. Atrichopogon riopardensis sp. nov. Male. A. Wing; B. Thorax, lateral view; C. Fore, mid- and hind legs (right to left), lateral view; D. Scutum, scutellum and postscutellum, dorsal view. FIGURE 9. Atrichopogon riopardensis sp. nov. Male. A. Abdomen, ventral view; B. Terminalia, lateral view; C. Terminalia, ventral view; D-E. Aedeagal-parameral complex, ventral view; F. Gonostylus. FIGURE 10. Atrichopogon riopardensis sp. nov. Female. A. Antenna; B. Head, anterior view; C. Adult, lateral view; D. Eyes separation, anterior view; E. Mandibular teeth; F. Third palpal segment. FIGURE 11. Atrichopogon riopardensis sp. nov. Female. A. Wing; B. Scutum, scutellum dorsal view; C. Spermatheca; D. Abdomen, ventral view; E. Fore, mid- and hind legs (left to right), lateral view. Type material. Holotype , adult male, slide-mounted, labelled as follows: BRAZIL , Amazonas , Presidente Figueiredo Municipality , Rio Pardo rural settlement, 1°49ʹ02.3″ S 60°19ʹ03.5″ W , CDC light trap , canopy forest, principal road, Pereira-Silva J. Col. VII. 2016 ( ILMD ) . Allotype , adult female, slide-mounted, same data as holotype ( ILMD ) . Paratypes : 3 males , same data as holotype, except Gusmão road, VIII. 2015 ( ILMD ) ; 2 males , same data as holotype, except for areas of transition between secondary capoeira and forest, F. Pessoa col. VII. 2010 ( ILMD ) ; 2 males , same data as holotype, except F. Pessoa col. VIII. 2010 ( ILMD ) . Etymology. This species is named in honor of the rural settlement of Rio Pardo, type locality, where Leônidas and Maria Deane Institute have a field base and have developed research activities since 2007.