A species-level taxonomic review and host associations of Glyptapanteles (Hymenoptera, Braconidae, Microgastrinae) with an emphasis on 136 new reared species from Costa Rica and Ecuador Author Arias-Penna, Diana Carolina Author Whitfield, James B. Author Janzen, Daniel H. Author Winifred Hallwachs, Author Dyer, Lee A. Author Smith, M. Alex Author Hebert, Paul D. N. Author Fernandez-Triana, Jose L. text ZooKeys 2019 890 1 685 http://dx.doi.org/10.3897/zookeys.890.35786 journal article http://dx.doi.org/10.3897/zookeys.890.35786 1313-2970-890-1 FD8F695311F64DF2950F6A387340BCE5 2691DADB7BA352BEBA377C901FC0AC97 Glyptapanteles felipesotoi Arias-Penna, sp. nov. Figs 82 , 83 Female. Body length 2.92 mm , antenna length 3.43 mm , fore wing length 3.38 mm . Type material. Holotype : ECUADOR • 1♀ ; EC-12321, YY-A122; Napo , Yanayacu Biological Station , Forest Aguilar , Plot 181; cloud forest; 2,241 m ; - 0.616667, -77.9; 17.ii.2006 ; Aaron Fox leg.; caterpillar collected in prepupa; cocoons formed on 07.iv.2006 ; adult parasitoids emerged on 12.iv.2006 ; ( PUCE ) . Paratypes . • 69 ( 5♀ , 6♂ ) ( 46♀ , 12♂ ); EC-12321, YY-A122; same data as for holotype; ( PUCE ) . Diagnosis. Distal half of propodeum rugose ( Figs 82F , 83C ), precoxal groove indistinct ( Figs 82I , 83E ), on pronotum central area smooth, but both dorsal and ventral furrows with short parallel carinae ( Figs 82C , 83E ), anterior furrow of metanotum without setiferous lobes ( Figs 82F , 83C ), petiole on T1 parallel-sided in proximal half, then narrowing ( Figs 82G , 83D ), edges of median area on T2 obscured by weak longitudinal stripes ( Figs 82G, H , 83D ), dorsal outer depression on hind coxa present ( Figs 82A, J , 83A, F ), and fore wing with r vein curved, outer side of junction of r and 2RS veins forming a stub ( Fig. 82K ). Figure 82. Glyptapanteles felipesotoi sp. nov. female EC-12321 YY-A122 A Habitus B, D Head B Frontal view D Dorsal view C Head, pronotum, propleuron, lateral view E Mesonotum, dorsal view F Scutellum, metanotum, propodeum, dorsal view G T1-3, dorsal view H, J Metasoma H Dorsal view J Lateral view I Mesosoma, lateral view K, L Wings K Fore L Hind. Figure 83. Glyptapanteles felipesotoi sp. nov. male EC-12321 YY-A122 A Habitus B Mesonotum, dorsal view C Scutellum, metanotum, propodeum, dorsal view D, F Metasoma D Dorsal view F Lateral view E Mesosoma, lateral view. Coloration ( Fig. 82 A-L ). General body coloration polished black except labrum and mandibles yellow-brown; glossa, maxillary and labial palps, and tegulae yellow; proximal ring on scape, and distal ring on pedicel reddish brown; all antennal flagellomeres brown on both sides. Eyes silver and ocelli reddish (in preserved specimen). Fore and middle legs yellow except tarsomeres, which coloration intensity increasing from proximal (yellow-brown) to distal (brown), and claws brown; hind legs yellow except black coxae, a tiny brown spot of femora, both ends of tibiae brown, and tarsomeres brown. Petiole on T1 black and sublateral areas yellow; T2 with median and adjacent areas black, and lateral ends yellow-brown; T3 mostly black, but with proximal corners yellow-brown; T4 and beyond completely black; distally each tergum with a narrow yellowish band. In lateral view, T1-2 completely yellow; T3 yellow, but dorsally brown; T4 and beyond brown. S1-4 yellow, medially brown, brown area increasing from proximal to distal; penultimate sternum and hypopygium completely brown. Description. Head ( Fig. 82 A-D ). Head rounded with pubescence long and dense. Proximal three antennal flagellomeres longer than wide (0.27:0.08, 0.29:0.08, 0.29:0.08), distal antennal flagellomere longer than penultimate (0.12:0.05, 0.10:0.05), antenna longer than body (3.43, 2.92); antennal scrobes-frons shallow. Face flat or nearly so, with dense fine punctations, interspaces smooth and longitudinal median carina present. Frons smooth. Temple wide, punctate and interspaces clearly smooth. Inner margin of eyes diverging slightly at antennal sockets; in lateral view, eye anteriorly convex and posteriorly straight. POL shorter than OOL (0.10, 0.14). Malar suture present. Median area between lateral ocelli slightly depressed. Vertex laterally rounded and dorsally wide. Mesosoma ( Fig. 82A, E, F, I ). Mesosoma dorsoventrally convex. Mesoscutum with narrow grooves/dents taking the place of notauli, punctation distinct throughout and interspaces smooth. Scutellum long and slender, apex of scutellum sloped and fused with BS , scutellar punctation distinct peripherally and absent centrally, in profile scutellum flat and on same plane as mesoscutum, phragma of the scutellum partially exposed; BS only very partially overlapping the MPM ; ATS demilune entirely covered by parallel carinae; dorsal ATS groove with carinae only proximally. Transscutal articulation with small and heterogeneous foveae, area just behind transscutal articulation smooth, shiny and nearly at the same level as mesoscutum (flat). Metanotum with BM wider than PFM (clearly differentiated); MPM semicircular and bisected by a median longitudinal carina; AFM without setiferous lobes and not as well delineated as PFM ; PFM thick, smooth and with a distal flat flange; ATM proximally with a groove with some sculpturing and distally smooth. Propodeum without median longitudinal carina, proximal half weakly curved with medium-sized sculpture and distal half rugose; distal edge of propodeum with a flange at each side and without stubs; propodeal spiracle without distal carina; nucha surrounded by very short radiating carinae. Pronotum with a distinct dorsal furrow, dorsally with a well-defined smooth band; central area smooth, but both dorsal and ventral furrows with short parallel carinae. Propleuron with fine punctations throughout and dorsally without a carina. Metasternum flat or nearly so. Contour of mesopleuron straight/angulate or nearly so; precoxal groove indistinct, smooth and shiny; epicnemial ridge elongated more fusiform (tapering at both ends). Legs. Ventral margin of fore telotarsus entire without seta, fore telotarsus almost same width throughout and longer than fourth tarsomere (0.17, 0.09). Hind coxa with dorsal half sparsely punctate, ventral half densely punctate, and dorsal outer depression present. Inner spur of hind tibia longer than outer spur (0.25, 0.20), entire surface of hind tibia with dense strong spines clearly differentiated by color and length. Hind telotarsus longer than fourth tarsomere (0.20, 0.15). Wings ( Fig. 82K, L ). Fore wing with r vein slightly curved; 2RS vein straight; r and 2RS veins forming an angle at their junction and outer side of junction forming a slight stub; 2M vein slightly curved/swollen; distally fore wing [where spectral veins are] with microtrichiae more densely concentrated than the rest of the wing; anal cell 1/3 proximally lacking microtrichiae; subbasal cell with microtrichiae virtually throughout; veins 2CUa and 2CUb completely spectral; vein 2 cu-a present as spectral vein, sometimes difficult to see; vein 2-1A proximally tubular and distally spectral, although sometimes difficult to see; tubular vein 1 cu-a curved, incomplete/broken and not reaching the edge of 1-1A vein. Hind wing with vannal lobe wide, subdistally and subproximally straightened, and setae present only proximally. Metasoma ( Fig. 82A, G, H, J ). Metasoma laterally compressed. Petiole on T1 finely sculptured only laterally, parallel-sided in proximal half and then narrow (length 0.45, maximum width 0.25, minimum width 0.15), and with scattered pubescence concentrated in the first distal third. Lateral grooves delimiting the median area on T2 clearly defined and reaching the distal edge of T2 (length median area 0.20, length T2 0.20), edges of median area obscured by weak longitudinal stripes, median area broader than long (length 0.20, maximum width 0.23, minimum width 0.12); T2 with scattered pubescence only distally. T3 longer than T2 (0.23, 0.20) and with scattered pubescence only distally. Pubescence on hypopygium dense. Cocoons. Unknown. Male ( Fig. 83 A-F ). The coloration on metasoma is darker than in females, but the shape is similar to female. Etymology. Felipe N. Soto-Adames is a Puerto Rican collembolan systematist. His interests are focused on insect systematics, phylogeny and evolution of Collembola , and evolution of arthropod muscle proteins. Currently, he is curator of Thysanoptera , Collembola , and non-Insect arthropods at the Florida State Collection of Arthropods in Gainesville , Florida , USA . Distribution. Parasitized caterpillar was collected in Ecuador , Napo , Yanayacu Biological Station (Forest Aguilar), during February 2006 at 2,241 m in cloud forest. Biology. The lifestyle of this parasitoid species is gregarious. Host. Memphis nr. lorna (Druce) ( Nymphalidae : Charaxinae ) feeding on Nectandra sp. ( Lauraceae ). Caterpillar was collected in prepupa.