Morphological and phylogenetic analyses reveal three new species and one new record of Tubeufia (Tubeufiales, Tubeufiaceae) from southern China Author Ma, Jian https://orcid.org/0009-0008-1291-640X School of Food and Pharmaceutical Engineering, Guizhou Institute of Technology, Guiyang 550003, China & Center of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai 57100, Thailand Author Zhang, Li-Juan https://orcid.org/0000-0002-3234-6757 School of Food and Pharmaceutical Engineering, Guizhou Institute of Technology, Guiyang 550003, China & Center of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai 57100, Thailand Author Boonmee, Saranyaphat https://orcid.org/0000-0001-5202-2955 Center of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai 57100, Thailand Author Xiao, Xing-Juan https://orcid.org/0009-0003-8769-4534 School of Food and Pharmaceutical Engineering, Guizhou Institute of Technology, Guiyang 550003, China Author Liu, Ning-Guo https://orcid.org/0000-0002-9169-2350 School of Food and Pharmaceutical Engineering, Guizhou Institute of Technology, Guiyang 550003, China Author Xiao, Yuan-Pin https://orcid.org/0000-0003-1730-3545 School of Food and Pharmaceutical Engineering, Guizhou Institute of Technology, Guiyang 550003, China Author Luo, Zong-Long https://orcid.org/0000-0001-7307-4885 School of Science, Mae Fah Luang University, Chiang Rai, 57100, Thailand Author Lu, Yong-Zhong https://orcid.org/0000-0002-1033-5782 School of Food and Pharmaceutical Engineering, Guizhou Institute of Technology, Guiyang 550003, China yzlu86@gmail.com text MycoKeys 2023 2023-08-14 99 87 108 http://dx.doi.org/10.3897/mycokeys.99.107606 journal article http://dx.doi.org/10.3897/mycokeys.99.107606 1314-4049-99-87 337967BE505158FEBE181E2FC931D128 Tubeufia hainanensis J. Ma & Y.Z. Lu sp. nov. Fig. 3 Etymology. The epithet '' Tubeufia hainanensis '' refers to the collecting site. Holotype. HKAS 125884. Description. Saprobic on decaying wood in moist ground. Sexual morph Undetermined. Asexual morph Hyphomycetous, helicosporous. Colonies on natural substrate superficial, effuse, gregarious, white. Mycelium superficial, partly immersed, hyaline to pale brown, septate, branched hyphae, smooth. Conidiophores macronematous, mononematous, straight to slightly flexuous, cylindrical, unbranched, septate, 44-56 μm long, 4-5 μm wide, pale brown to hyaline, smooth-walled. Conidiogenous cells holoblastic, mono- to polyblastic, integrated, sympodial, intercalary or terminal, cylindrical, with a denticulate protrusion, truncate at apex after conidial secession, 8-14 μm long, 3-5 μm wide (x̄= 9.5 x 4 μm , n = 20), hyaline to pale brown, smooth-walled. Conidia solitary, acropleurogenous, helicoid, rounded at tip, 16-21 μm diam conidial filament 1.5-4 μm wide (x̄ = 19 x 3 μm , n = 30), 127-175 μm long (x̄ = 144 μm , n = 30), indistinctly septate, coiled 31/2-33/4 times, becoming uncoiled in water, guttulate, hyaline, smooth-walled. Figure 3. Tubeufia hainanensis (HKAS 125884, holotype) a, b colonies on the host surface c-e conidiophores, conidiogenous cells and conidia f-h conidiogenous cells bearing conidia j-o conidia i germinated conidium p, q colonies on PDA, p from above q from below. Scale bars: 20 μm ( c-e, i-l ); 10 μm ( f-h, m-o ). Culture characteristics. Conidia germinating on water agar and producing germ tubes within 8 h. Colonies on PDA circular with umbonate surface and undulate edge. Growth rate 43 mm diam in 50 days at 25 °C, with a pale brown surface. Material examined. China , Hainan Province , Haikou City , Xiuying District , Ecological leisure trail, 20°01′N , 110°25′E , on decaying wood in terrestrial habitat, 10 August 2021 , Jian Ma , HK 1 (HKAS 125884, holotype ; GZAAS 22-2015, isotype), ex-type living cultures CGMCC, GZCC 22-2015; Ibid. , HK 1-2 (HKAS 125883, paratype ), living culture GZCC 23-0589 . Notes. Tubeufia hainanensis resembles T. parvispora morphologically, with solitary, acropleurogenous, hyaline, helicoid conidia. However, Tubeufia hainanensis can be distinguished from T. parvispora by its unbranched conidiophores ( Lu et al. 2018b ). Tubeufia hainanensis formed a sister clade to Tubeufiaceae (BBC 3512) with 85% ML/0.99 PP supports (Fig. 1 ), and the phylogeny indicated that it is distinct species.