A king-sized theropod coprolite Author Karen Chin United States Geological Survey, 345 Middlefield Road, MS 975, Menlo Park, California 94025, USA Author Timothy T. Tokaryk Eastend Fossil Research Station, Royal Saskatchewan Museum, Box 460, Eastend, Saskatchewan S 0 N 0 T 0, Canada Author Gregory M. Erickson Department of Integrative Biology & Museums of Vertebrate Zoology and Paleontology, University of California, Berkeley, California 94720, USA Author Lewis C. Calk United States Geological Survey, 345 Middlefield Road, MS 975, Menlo Park, California 94025, USA text Nature 1998 1998-06-18 393 680 682 journal article 21498 10.1038/31461 7e32cdfa-1a01-4a80-8b35-3c3e6d946e38 3943146 Fossil faeces (coprolites) provide unique trophic perspectives on ancient ecosystems. Yet, although thousands of coprolites have been discovered, specimens that can be unequivocally attributed to carnivorous dinosaurs are almost unknown. A few fossil faeces have been ascribed to herbivorous dinosaurs 1 – 3 , but it is more difficult to identify coprolites produced by theropods because other carnivorous taxa coexisted with dinosaurs and most faeces are taxonomically ambiguous. Thus sizeable (up to 20 cm long and 10 cm wide) phosphatic coprolites from Belgium 4 and India 5 , 6 that have been attributed to dinosaurs might have been produced by contemporaneous crocodylians 7 or fish. But there is no ambiguity about the theropod origin of the Cretaceous coprolite we report here. This specimen is more than twice as large as any previously reported carnivore coprolite, and its great size and temporal and geographic context indicate that it was produced by a tyrannosaur, most likely Tyrannosaurus rex . The specimen contains a high proportion (30–50%) of bone fragments, and is rare tangible evidence of theropod diet and digestive processes. The specimen ( SMNH P2609.1) was discovered as an elongate mass weathering out of the fluvial Maastrichtian Frenchman Formation in Southwestern Saskatchewan, roughly 35 km southeast of the town of Eastend . The fractured mass was distinguished by its indurated nature and numerous inclusions of comminuted bone. The main portion of the mass was found in situ in a bentonitic mudstone, though numerous fragments had eroded downslope. No fossil bones were found in association with the coprolite, but fossils of a number of large vertebrates have been recovered from the Frenchman Formation 8 . Figure 1 Large, bone-bearing theropod coprolite with some of the broken pieces that had eroded downslope. This specimen was found in Chamberry Coulee in the Frenchman River Valley, roughly 11.5 m below the Cretaceous/Tertiary boundary. Scale bar,10 cm. The main body of the specimen is roughly 44 cm long, 13 cm high and 16 cm wide ( Fig. 1 ). The density of the material (approximately 2.94 g ml 1) and the weight of all portions (over 7.1 kg) indicate that the present volume of the mass is 2.4 litres, though it is likely that the original faecal mass was larger before it was subjected to compaction, attrition, and/or desiccation. Broken surfaces of the specimen expose numerous dark brown macroscopic bone fragments ranging from 2 to 34 mm in length. These pieces are suspended in a microcrystalline ground mass and are generally aligned in a consistent direction. The ground mass also contains sand-sized bone clasts ( Fig. 2 ). Most of the included bone appears to be similar in type, with highly vascularized cortical bone tissue up to 14-mm thick in a fibrolamellar pattern. All of the observed bone is primary, and no lines of arrested growth were detected. Figure 2 Photomicrograph of a thin section of the theropod coprolite, showing sand- to pebble-sized bone clasts within a microcrystalline phosphatic ground mass.The elemental composition of the ground mass is similar to that of the bone fragments,indicating that it is probably largely composed of reprecipitated bone apatite infiltrated by clay minerals from the host sediment ( Table 1 ). The large bone fragment in the upper left portion of the image exhibits a fibrolamellar pattern, with osteocyte lacunae concentrically arranged around the vascular canals.Probe measurements of the interior of bone lacunae revealed that many of these channels are at least partially empty, whereas others exhibit variable element distributions, with generally lower concentrations of calcium and phosphorus,and higher silicon and aluminium levels ( Table 2 ).Scale bar, 400 m. Bulk chemical analyses using X-ray fluorescence ( Table 1 ) reveal marked differences between the specimen and the Frenchman Formation mudstone. The bone-bearing specimen contains high concentrations of phosphorus and calcium, and lower concentrations of aluminium and silicon, relative to the host sediment. Microprobe analyses of specific areas of the specimen indicate that the bone fragments and coprolitic ground mass have similar compositions, though the ground mass contains more silicon and aluminium ( Table 2 ). X-ray powder-diffraction analyses indicate that carbonate fluorapatite is the predominant phosphate mineral in both the bone and the ground mass. Several factors confirm that this specimen is a coprolite. The most diagnostic feature is a phosphatic composition, which is characteristic of carnivore coprolites 9 . As phosphorus normally constitutes only about 0.1% of the Earth’s crustal rocks 10 , concentrated phosphate deposits usually indicate biotic accumulations, and the overall configuration of the mass is consistent with the irregular faecal deposits produced by very large animals. The matrix-supported distribution of bone fragments argues against the possibility that the mass represents regurgitated material or fluvially aggregated bone debris. The tremendous size of the specimen indicates that the faecal mass was produced by a large theropod. The largest theropod found in the Frenchman Formation is Tyrannosaurus, with an estimated body weight of 5,400 to 6,300 kg (ref. 11). Although other theropods, crocodylomorphs, and a chelonian (Dromaeosaurus, Saurornitholestes, Troodon, Richardoestesia, Chirostenotes, an ornithomimid, Leidyosuchus, Champsosaurus, and Aspideretes) have also been recovered from the Frenchman Formation 8 , these smaller carnivorous taxa probably weighed 100 kg (ref. 12), and it is unlikely that they could have produced large quantities of faeces. The mass could have been produced by a different species of tyrannosaur, but no others have been recognized in the Frenchman Formation. ‡ Present address: Biomechanical Engineering Division, Mechanical Engineering Department & Rehabilitation R & D Center, VA Health Care System, Stanford University, Stanford, California 94305, USA. letters to nature The stomach acids and proteolytic enzymes of large extant carnivores digest bone to varying degrees 13 – 16 . Tangible evidence of this process is apparent in areas of the theropod coprolite where aligned and rounded bone pieces represent the degraded remains of large bone fragments ( Fig. 3 ). The contents of carnivore coprolites might reflect animal physiology, because the extent of bone digestion can be indicative of gut-residence time 17 . Carnivore feeding activity is usually variable, however, and stomach acidity and gutresidence time can be altered by non-physiological factors such as frequency of meals 15 . Even so, the high percentage of incompletely digested bone in this specimen is interesting because it is inconsistent with the general prediction that large theropods digested most consumed bone 18 in the manner of extant crocodilians 14 . The chemistry of the coprolite reflects several factors. A large percentage of the phosphate of the ground mass was probably derived from dissolved bone apatite, but other dietary residues would have contributed additional phosphorus, as microorganisms and animal soft tissues contain significant concentrations of this element. Postdepositional phosphate precipitation may have been triggered by bacterial enzymes 19 after burial of the faecal mass. Although the chemistry of this diagenetic phosphate is similar to that of the included bone, the increased amounts of silicon and aluminium and small differences in amounts of other elements indicate contributions from the host sediment. Thus, although the overall phosphatic composition of the coprolite reflects a carnivorous diet, minor chemical differences in the bone and ground mass seem to reveal more about diagenesis than about diet. Histological and morphological analyses of the included bone fragments give clues to the identity of the ingested animal. Dinosaurs are the only Late Cretaceous animals that regularly produced thick fibrolamellar cortical bone 20 . The absence of secondary osteons indicates that the bone was ontogenetically juvenile, so the ingested animal appears to have been a subadult dinosaur. Although bone histology is not species-specific, the absence of arrested growth lines may indicate an ornithischian dinosaur. Lines of arrested growth have been observed in several theropods 21 but have Edmontosaurus not been (G.M.E observed., in unpublished the long bones observation of), Triceratops the most and8 common dinosaurs found in the Frenchman Formation. Other ornithischians from the formation include Torosaurus, Thescelosaurus and an ankylosaur 8 . The thickness of the cortical bone indicates that the fragments may be derived from appendicular bone or ceratopsian frill. If the fragments were derived from long-bone diaphyses, estimates of the weight 22 of the consumed animal might range from 200 kg (for a bipedal dinosaur) to 750 kg (for a quadrupedal dinosaur). The pronounced fragmentation and angularity of the consumed bone indicate that it was fractured before ingestion—apparently by biting during feeding. Although extant birds (avian dinosaurs) often use a horny gizzard and/or ingested grit for food maceration 23 , such mechanisms could not have been solely responsible for the degree of comminution seen in the coprolite. Furthermore, significant trituration would have resulted in well rounded bone clasts, and there is no evidence for the use of gastroliths by non-avian theropods. The coprolitic evidence for extensive bite-induced bone fragmentation is surprising in view of modern reptilian feeding habits. Extant reptiles have poor dental occlusion and generally swallow large pieces of prey whole 14 , 24 . Such observations of modern feeding behaviours have led to speculation that extinct theropods did little bone-crushing 18 , 25 and wasted a significant proportion of the food available from carcasses 26 . Tyrannosaur teeth appear to be stout enough to damage bone 27 , however, and analyses of bite marks on Triceratops and Edmontosaurus bones indicate that Tyrannosaurus pulverized bones during feeding 28 and probably consumed bone fragments 29 . Table 1 X-ray-fluorescence data of the weight percentage of oxides found in bulk powdered samples of the coprolite and host sediment
Coprolite Host sediment .............................................................................................................................................................................
SiO2 7.93 70.2
Al2O3 2.43 18.1
TiO2 0.128 0.794
FeO 0.95 6.02
MnO 0.151 0.018
CaO 44.6 0.97
MgO 0.16 1.42
K2O 0.27 2.22
Na2O2 0.28 1.08
P2O5 26.5 0.70
Total 83.5 100.9 .............................................................................................................................................................................
Figure 3 Photomicrograph of a thin section of the theropod coprolite, showing associated bone fragments that indicate digestive degradation. Digestive acids and enzymes probably infiltrated the interior of the bone through vascular canals. Scale bar,100 m. The clay-rich sediment reflects the low phosphorus concentration of most inorganic rocks, whereas the coprolite is largely composed of biotically concentrated phosphorus and calcium. Table 2 Microprobe data indicating weight percentage of oxides, fluorine and chlorine in different regions of the coprolite 0.038 0.094 0.484 0.230 0.103 51.5 0.157 0.335 0.025 0.142 35.7 2.95 NA 90.5 0.582 90.7 0.584 82.4 4.96 ...................................................................................................................................................................................................................................................................................................................................................................
Bone fragments n ¼ 60 points Ground mass n ¼ 60 points Bone lacunae n ¼ 27 points
Mean s.d. Mean s.d. Mean s.d.
...................................................................................................................................................................................................................................................................................................................................................................
SiO2
0.102 4.03 1.42 29.5 13.2
Al2O3 0.046 1.71 0.516 13.6 5.80
FeO 0.034 0.730 0.101 3.24 1.39
MnO 0.026 0.144 0.018 0.068 0.046
MgO 0.018 0.210 0.030 1.02 0.47
CaO 0.290 48.9 1.09 20.2 13.5
SrO 0.030 0.117 0.024 0.036 0.040
Na2O 0.046 0.237 0.032 0.184 0.056
K2O 0.010 0.163 0.044 0.312 0.110
SO3 0.055 0.285 0.029 0.157 0.080
P2O5 0.466 32.6 0.859 13.4 9.37
F 0.074 2.62 0.117 0.943 0.750
Cl — NA — 0.108 0.090
Total
The compositions of the bone and ground mass are similar, though the ground mass appears to contain more contributions from the host sediment.Of 67 probe measurements of lacunae, 40 registered low element totals, indicating that the vascular canals were incompletely filled. These channels would have acted as conduits for digestive fluids and for postdepositional contaminants. Data listed above are from the 27 lacunae that registered element totals over 70%. NA, no analyses done. Although a single coprolite cannot be construed as representative of diet, this rare example of fossilized dietary residues helps to refine our understanding of theropod feeding behaviour by providing physical evidence that a tyrannosaur crushed, consumed, and incompletely digested large quantities of bone when feeding on a subadult dinosaur. It also presents a new search image for future discoveries of theropod faeces that will help us to elucidate the food habits of these giant meat-eaters.