DNA barcoding confirms the validity of Anthidium melanopygum Friese, 1917 stat. nov. (Hymenoptera: Megachilidae) as a distinct species of Western Asia Author Kasparek, Max Mönchhofstr. 16, 69120 Heidelberg, Germany. Author Fateryga, Alexander V. T. I. Vyazemsky Karadag Scientific Station-Nature Reserve of RAS-Branch of A. O. Kovalevsky Institute of Biology of the Southern Seas of RAS, Nauki str. 24, Kurortnoye vill., 298188 Feodosiya, Crimea. text Zootaxa 2023 2023-09-21 5346 5 567 580 http://dx.doi.org/10.11646/zootaxa.5346.5.4 journal article 270782 10.11646/zootaxa.5346.5.4 e8414ca7-9f8e-4120-9bca-6167a5dc6aa8 1175-5326 8390786 CD9FE607-CDEF-4690-B401-F8AD0BADB167 Anthidium melanopygum Friese, 1917 stat. nov. Anthidium spiniventre var. melanopygum Friese, 1917: 56 , ♀ . Type locality: “ Kleinasien (Asia minor), Taurus cilic.” [ Turkey ]; holotype , ♀ : “Asia min. 1890” (ZMB; photographs examined). Material examined: AZERBAIJAN ( Nakhchivan Autonomous Republic): Shakhbuz , Shakhbuzkend ( 39°23′N , 45°32′E ), 1140 m , 22.vii.2018 , 2♁, M. Proshchalykin, Kh. Aliyev, M. Maharramov leg. (coll. Proshchalykin; Fateryga et al. , 2020).— Babek , Sirab, 22.vi.2012 , 1♀ , Kh. Aliyev leg. (Institute of Zoology of the National Academy of Sciences of Azerbaijan; Fateryga et al. , 2020).— Babek , 3 km NE of Sirab ( 39°18′N , 45°32′E ), 1250 m , 21.vi.2019 , 3♀ , M. Proshchalykin, Kh. Aliyev, M. Maharramov leg. (coll. Proshchalykin; Fateryga et al. , 2020).— Babek , Goynuk ( 39°18′N , 45°40′E ), 1680 m , 12.vi.2019 , 2♀ , 2♁, M. Proshchalykin, Kh. Aliyev, M. Maharramov leg. (coll. Proshchalykin; Fateryga et al. , 2020).— Julfa , Daridagh ( 38°59′N , 45°40′E ), 900 m , 16.vi.2019 , 1♀ , M. Proshchalykin, Kh. Aliyev, M. Maharramov leg. (coll. Proshchalykin; Fateryga et al. , 2020).— Ordubad (Arax [= Aras]), 1892, Reitter leg., 1♀ ( ZISP ; Fateryga et al. , 2020).— Kangarli , Chalkhangala ( 39.4166°N , 45.2166°E ), 25.vi.2020 , 1♀ , 1♁, Maharramov leg. (coll. Proshchalykin; COI ).— BULGARIA : Sandanski, 12.–26.vii.1966 , 7♀ , 11♁, M. Kocourek leg. ( CMK ).— GREECE : Aroania, 33 lm SW Kalavrita, 1000 m , 02.viii.1976 , 1♀ , A.W. Ebmer leg. (priv. coll. Ebmer).—Chios, SW Pityos ( 38.28°N , 26.02°E ), 470 m , 18.vi.2007 , 1♀ , A.W. Ebmer leg. (priv. coll. Ebmer).—Thessaloniki: Hortiatis ( 40.593°N , 23.093°E ), 08.vii.2019 , 1♁, M. Halada leg. ( CMK ; COI ).— Crete : 1.vii.1925 , at least 3 ex. ( ZMB ; drawer photographs examined).— Crete : Weisse Bge., Lakki, 500 m , 10.vi.1942 , 1♁, Kl. Zimmermann leg. ( ZMB ; photographs examined).— Crete : Asfendos ( 35°14′28′′N , 24°12′54′′E ), 700 m , 13.vi.2002 , 1♀ , A.W. Ebmer leg. (priv. coll. Ebmer).— Crete : betw. Vorizia and Varsamonero ( 35.08°N , 24.51°E ), 450–500 m , 20.v.2003 , 1♀ , A.W. Ebmer leg. (priv. coll. Ebmer).—Loutraki, Golf von Korinth, 25.v.1962 , 1♀ , M. Schwarz leg. ( CMK ).—Peloponesse, Oitylo env. ( 36.6970°N , 22.3893°E ), 25 m , 05.vii.2019 , 1♁, P. Bogusch leg. ( CMK ; COI ).—Peloponnesse, Mt. Tayjetos Oros centr., 28.–31.vii.2010 , 2♀ , J. Halada leg. ( CMK ).—Platania / Volos, 26.vi.2008 , 1♁, K. Standfuss leg. (coll. Fuhrmann).— IRAN : Alborz : Chalus, road-Shahrestanak ( 35°57.583′N , 51°22.339′E ), 18.vii.2010 , 1♀ , A. Nadimi leg. (coll. Nadimi; Nadimi et al. , 2014, as “ Anthidium cf. spiniventre ”; photographs examined).— Alborz : Ab Ali, 09.–12.vi.1965 , 1♁, G. Soika & G.A. Mavromoustakis leg. ( OLL 1109; Warncke, 1982).— Alborz : Above Karaj Lake, 12.vii.1977 , 1♀ aff. melanopygum , J. Gusenleitner leg. ( OLL 1101; attributed to melanopygum by Warncke, 1982).— Alborz : Torrente, 27 km N Karadj, 1♀ aff. melanopygum , G. Soika & G.A. Mavromoustakis leg. ( OLL 1103; Warncke, 1982).—Vana, 50 km N Ab Ali, 12.–13.vii.1965 , 2♀ ( OLL 1102 aff. A. melanopygum , OLL1105 A. melanopygum ; both as ssp. melanopygum in Warncke, 1982).— Golestan : 45 km E Minudasht ( 37°36′N , 55°93′E), 11.vi.2010 , 1♀ , Mi. Halada leg. ( CMK ).— Fars : 18 km N Shiraz, 1♁, 06.vii.1965 , G. Soika & G.A. Mavromoustakis leg. ( OLL 1108; Warncke, 1982).— Fars : 20 km N Shiraz, 4.vii.1965 , 1♀ , G. Soika & G.A. Mavromoustakis leg. [ aff. melanopygum ] ( OLL 1107; Warncke, 1982).— Fars : Pass E Neyriz and 20 km W Neyriz, 18.v.1978 , 1♀ , 3♁, K. Warncke leg. ( OLL 1110–12; Warncke, 1982).— Semnan prov. , Shahmirzad 13 km NW ( 35°52′11″N , 53°12′46″E ), 1836 m , 07.vii.2022 , 1♀ , 3♁, Ch. Schmid-Egger & W.-H. Liebig leg. ( CSE : seg333, 336, 337; coll. Liebig: whl150).—Razawi-Khorasan prov., Binaluth Mt., 15 km SW Shandiz ( 36.2929°N , 59.1384°E ), 1931 m , 19.vii.2022 , 1♁ ( CSE : seg334).—Khorasan-North prov., Bajgiran 27 km SE ( 37.6991°N , 58,1002°E ), 2194 m , 12.vii.2022 , 1♁ ( CMK : seg335).— Teheran prov. , Elburz , Firuzkuh 1 km SW ( 35°43′38″N , 52°45′10″E ), 1879 m , 06.vii.2022 , 1♀ , 1♁, W.-H. Liebig & Ch. Schmid-Egger leg. ( CSE : seg338; coll. Liebig: whl151). — Teheran : Dashteban 1 km W ( 35°44′38″N , 52°02′38″E ), 2225 m , 05.vii.2022 , 1♀ , W.-H. Liebig leg. (coll. Liebig: whl152).— LEBANON : North Liban: Tannourine Forest Reserve, main gate ( 34°12′25′′N , 35°55′59′′E ), 1792 m , 13.vii.2017 , 1♀ , M. Boustani leg. (coll. Boustani; COI ).— RUSSIA ( Dagestan ): Derbent Distr.: Kamyshchay River valley ( 41.9091°N , 48.2297°E ), 21.vi.2018 , 1♀ , A. Fateryga leg. (coll. Fateryga; Fateryga et al. , 2019; COI ), 29.vi.2018 , 1♁, M. Proshchalykin, V. Loktionov, M. Mokrousov leg. (coll. Proshchalykin; Fateryga et al. , 2019).—Makhachkala Distr.: Talgi env. ( 42.8766°N , 47.4450°E ), 25.vi.2018 , 4♀ , 5♁, M. Proshchalykin, V. Loktionov, M. Mokrousov leg. (1♁ coll. Fateryga, 4♀ , 4♁ coll. Proshchalykin; Fateryga et al. 2019; 1 COI ), 3♁ Yu. Astafurova leg. ( ZISP ; Fateryga et al. 2019).— TURKEY : Taurus Mountains (Cilician Taurus) (type locality), 1890, female holotype ( ZMB ; Friese, 1917; photographs examined).— Adana , S Karanfil Dağı, E Kamışlı ( 37°32′31′′N , 34°59′05′′E ), 1500 m , 09.vii.1995 , 2♁, P. Rasmont leg. ( UMONS ).— Adana : Pozanti, 03.–06.vii.1983 , 1♁, J. Halada leg. ( CMK ).— Adıyaman : Gölbaşı, 21.vi.1985 , 2♀ , 2♁, M. Schwarz leg. ( CMK ).— Adıyaman : Nemrut Dağı, Karadut, 02.vii.1993 , 1♁, K. Denes leg. ( CMK ).— Ankara : Beynam, Atatürk Forest road ( 39°41′05′′N , 32°54′22′′E ), 1191 m , 07.vii.1998 , 1♀ , M. Aytekin & M. Campo ( UMONS ).— Ankara : Elma Dağı, 27.vi.1962 , 1♁, K. M. Guichard & D. H. Harvey leg. ( NHMUK ).— Ankara : Kavaklıdere, 25.vi.1962 , 1♀ , K. M. Guichard & D. H. Harvey leg. ( NHMUK ).—Antakya env., 30.iv.1994 , 1♀ , Mi. Halada leg. ( CMK ).— Burdur : 10 km W Yeşilova, Salda Gölü ( 37°32′N , 29°40′E ), c. 1000 m , 22.vi.2002 , 1♀ , G. Hölzler leg. (coll. Hölzler).— Burdur : 5 km NE Yeşilova ( 37°35′N , 29°55′E ), 1060 m , 06.vii.2006 , 1♁, J. Halada leg. ( CMK ).— Elazığ , SE (Hazar Gölü), 29.vi.2000 , 1♀ , 2♁, M. Halada leg. ( CMK ).— Hakkari : Hakkari-Van road, 5 mi N of Hakkari , 15.vii.1971 , 1♁, 1220 m (Leicester University; NHMUK ).—Beyetüşşebap, 19 km S, 1200 m , 26.vi.1985 , 3♀ , 5♁, M. Schwarz leg. ( CMK ).— Isparta : 8 km N Isparta ( 37°52′N , 30°40′E ), 1020 m , 09.vii.2006 , 1♁, J. Halada ( CMK ).— Konya : Karaman 10 km S, 19.vi.1985 , 1♁, M. Schwarz leg. ( CMK ).—Sille near Konya , 4.vii.1977 , 1♁, J. Heinrich leg. ( SMF ).— Mardin : 20 km N, 21.vi.1997 , 2♀ , 1♁, Ma. Halada ( CMK ).— Mersin : Ekşiler (Silifke), 27.v.1998 , 1♀ , Ma. Halada leg. ( CMK ).— Muğla env., 15.vii.2017 , 1♀ , 3♁, M. Kasparek leg. ( CMK ; 3 COI ).— Muğla prov. : Kozağaç, 14.vii.2017 , 1♀ , 1♁, M. Kasparek leg. ( CMK ; 1 COI ).— Urfa : 20 km N Ceylanpınar, 500 m , 18.vi.1981 , 1♀ , K. Warncke leg. ( CMK ). Additional literature records: AZERBAIJAN : Xanlar (= Göygöl = Helendorf ) ( Warncke , 1980) .— GREECE : Central Macedonia : Mount Olympus ( 40.0947°N , 22.3690°E ), 2596 m , 14.iv.2016 , 1♁, Melissotheque database ( Ascher & Pickering , 2022) .— Crete : Lato / Ag. Nikolaso , Anogia Heraklion and Sitia ( Warncke , 1980).— Evros : Dadia (40.9874°N, 26.0582°), 1♀ ,Melissotheque database ( Ascher & Pickering , 2022).— Chios:Vessa 2( 38.2762°N , 26.0163°E ), 1♀ , Melissotheque database ( Ascher & Pickering , 2022).— Thesssaly : Magnisia , 1999–2002, 7♁ ( Standfuss et al. , 2003) .— TURKEY : Ankara : Şereflikochisar ( 70 km S of Ankara ) ( Warncke , 1980) .— Erzurum , 1♁ 25.vii. 1983 and 1♁ 20.viii.1993 ( Özbek & van der Zanden , 1993) .— Isparta : Gönen (37°58′36′′N, 30°30′95′′E), 1180 m , 04.viii.2009 , 1♀ ( Güler et al. , 2014) .— Konya : Konya (town), Madenşehir, and Sille ( Warncke , 1980) .— Nevşehir : Göreme , 10.vii.1974 , 1♀ ( Özbek & van der Zanden , 1993) .— TURKMENISTAN : Repetek ( Warncke , 1980) .— UZBEKISTAN : Mentioned by Fateryga et al. (2020) based on Warncke’s (1980) statement “Repetek/ Buchara” (Repetek is situated in Turkmenistan, Buchara in Uzbekistan ), but no record from Uzbekistan .