A revision of European species of the genus Tetrastichus Haliday (Hymenoptera: Eulophidae) using integrative taxonomy Author Hansson, Christer The Natural History Museum, London, United Kingdom & Biological Museum (Entomology), Lund University, Lund, Sweden christerdennis@gmail.com Author Schmidt, Stefan https://orcid.org/0000-0001-5751-8706 SNSB-Zoologische Staatssammlung Muenchen, Munich, Germany schmidt.s@snsb.de text Biodiversity Data Journal 2020 2020-12-16 8 59177 59177 http://dx.doi.org/10.3897/BDJ.8.e59177 journal article http://dx.doi.org/10.3897/BDJ.8.e59177 1314-2828-8-e59177 AD70B3AB67634D2885F290988225C5C8 2BC7CCC36D765F1C87ACBE8025DFD848 Tetrastichus argei sp. n. Materials Type status: Holotype . Occurrence: occurrenceDetails: http://www.boldsystems.org/index.php/API_Public/specimen?ids=BC-ZSM-HYM-20721-E05; catalogNumber: BC-ZSM-HYM-20721-E05 ; recordNumber: BC-ZSM-HYM-20721-E05; recordedBy: C. Hansson ; individualID: BC-ZSM-HYM-20721-E05; individualCount: 1 ; sex: female ; lifeStage: adult ; Taxon: scientificName: Tetrastichusargei; phylum: Arthropoda; class: Insecta; order: Hymenoptera; family: Eulophidae; genus: Tetrastichinae; taxonRemarks: Holotype deposited in MZLU; Location: country: Sweden ; decimalLatitude: 55.683 ; decimalLongitude: 13.5 ; Record Level: type: PhysicalObject; language: en; institutionCode: MZLU ; basisOfRecord: PreservedSpecimen Type status: Paratype . Occurrence: occurrenceDetails: http://www.boldsystems.org/index.php/API_Public/specimen?ids=BC-ZSM-HYM-22523-C07; catalogNumber: BC-ZSM-HYM-22523-C07 ; recordNumber: BC-ZSM-HYM-22523-C07; recordedBy: Swedish Malaise Trap Project ; individualID: BC-ZSM-HYM-22523-C07; individualCount: 1 ; sex: female ; lifeStage: adult ; Taxon: scientificName: Tetrastichusargei; phylum: Arthropoda; class: Insecta; order: Hymenoptera; family: Eulophidae; genus: Tetrastichinae; Location: country: Sweden ; decimalLatitude: 57.322 ; decimalLongitude: 18.203 ; Record Level: type: PhysicalObject; language: en; institutionCode: SMTP ; basisOfRecord: PreservedSpecimen Type status: Paratype . Occurrence: occurrenceDetails: http://www.boldsystems.org/index.php/API_Public/specimen?ids=BC-ZSM-HYM-21587-A01; catalogNumber: BC-ZSM-HYM-21587-A01 ; recordNumber: BC-ZSM-HYM-21587-A01; recordedBy: C.Hansson ; individualID: BC-ZSM-HYM-21587-A01; individualCount: 1 ; sex: female ; lifeStage: adult ; Taxon: scientificName: Tetrastichusargei; phylum: Arthropoda; class: Insecta; order: Hymenoptera; family: Eulophidae; genus: Tetrastichinae; Location: country: Sweden ; decimalLatitude: 56.633 ; decimalLongitude: 16.623 ; Record Level: type: PhysicalObject; language: en; institutionCode: MZLU ; basisOfRecord: PreservedSpecimen Type status: Paratype . Occurrence: occurrenceDetails: http://www.boldsystems.org/index.php/API_Public/specimen?ids=BC-ZSM-HYM-22526-F12; catalogNumber: BC-ZSM-HYM-22526-F12 ; recordNumber: BC-ZSM-HYM-22526-F12; recordedBy: S. Schmidt ; individualID: BC-ZSM-HYM-22526-F12; individualCount: 1 ; sex: female ; lifeStage: adult ; Taxon: scientificName: Tetrastichusargei; phylum: Arthropoda; class: Insecta; order: Hymenoptera; family: Eulophidae; genus: Tetrastichinae; Location: country: Sweden ; decimalLatitude: 56.609 ; decimalLongitude: 16.508 ; Record Level: type: PhysicalObject; language: en; institutionCode: ZSM ; basisOfRecord: PreservedSpecimen Type status: Paratype . Occurrence: occurrenceDetails: http://www.boldsystems.org/index.php/API_Public/specimen?ids=BC-ZSM-HYM-25461-C06; catalogNumber: BC-ZSM-HYM-25461-C06 ; recordNumber: BC-ZSM-HYM-25461-C06; recordedBy: P. Benander ; individualID: BC-ZSM-HYM-25461-C06; individualCount: 1 ; sex: female ; lifeStage: adult ; Taxon: scientificName: Tetrastichusargei; phylum: Arthropoda; class: Insecta; order: Hymenoptera; family: Eulophidae; genus: Tetrastichinae; Location: country: Sweden ; decimalLatitude: 56.294 ; decimalLongitude: 12.495 ; Record Level: type: PhysicalObject; language: en; basisOfRecord: PreservedSpecimen Type status: Paratype . Occurrence: occurrenceDetails: http://www.boldsystems.org/index.php/API_Public/specimen?ids=BC-ZSM-HYM-27493-G09; catalogNumber: BC-ZSM-HYM-27493-G09 ; recordNumber: BC-ZSM-HYM-27493-G09; recordedBy: SMTP ; individualID: BC-ZSM-HYM-27493-G09; individualCount: 1 ; sex: female ; lifeStage: adult ; Taxon: scientificName: Tetrastichusargei; phylum: Arthropoda; class: Insecta; order: Hymenoptera; family: Eulophidae; genus: Tetrastichinae; Location: country: Sweden ; decimalLatitude: 64.114 ; decimalLongitude: 19.363 ; Record Level: type: PhysicalObject; language: en; institutionCode: SMTP ; basisOfRecord: PreservedSpecimen Type status: Paratype . Occurrence: occurrenceDetails: http://www.boldsystems.org/index.php/API_Public/specimen?ids=BC-ZSM-HYM-25461-C07; catalogNumber: BC-ZSM-HYM-25461-C07 ; recordNumber: BC-ZSM-HYM-25461-C07; recordedBy: P. Benander ; individualID: BC-ZSM-HYM-25461-C07; individualCount: 1 ; lifeStage: adult ; Taxon: scientificName: Tetrastichusargei; phylum: Arthropoda; class: Insecta; order: Hymenoptera; family: Eulophidae; genus: Tetrastichinae; Location: country: Sweden ; decimalLatitude: 56.294 ; decimalLongitude: 12.495 ; Record Level: type: PhysicalObject; language: en; basisOfRecord: PreservedSpecimen Type status: Paratype . Occurrence: occurrenceDetails: http://www.boldsystems.org/index.php/API_Public/specimen?ids=BC-ZSM-HYM-25461-C08; catalogNumber: BC-ZSM-HYM-25461-C08 ; recordNumber: BC-ZSM-HYM-25461-C08; recordedBy: P. Benander ; individualID: BC-ZSM-HYM-25461-C08; individualCount: 1 ; lifeStage: adult ; Taxon: scientificName: Tetrastichusargei; phylum: Arthropoda; class: Insecta; order: Hymenoptera; family: Eulophidae; genus: Tetrastichinae; Location: country: Sweden ; decimalLatitude: 56.294 ; decimalLongitude: 12.495 ; Record Level: type: PhysicalObject; language: en; basisOfRecord: PreservedSpecimen Type status: Paratype . Occurrence: occurrenceDetails: http://www.boldsystems.org/index.php/API_Public/specimen?ids=BC-ZSM-HYM-25461-C09; catalogNumber: BC-ZSM-HYM-25461-C09 ; recordNumber: BC-ZSM-HYM-25461-C09; recordedBy: P. Benander ; individualID: BC-ZSM-HYM-25461-C09; individualCount: 1 ; lifeStage: adult ; Taxon: scientificName: Tetrastichusargei; phylum: Arthropoda; class: Insecta; order: Hymenoptera; family: Eulophidae; genus: Tetrastichinae; Location: country: Sweden ; decimalLatitude: 56.294 ; decimalLongitude: 12.495 ; Record Level: type: PhysicalObject; language: en; basisOfRecord: PreservedSpecimen Type status: Paratype . Occurrence: occurrenceDetails: http://www.boldsystems.org/index.php/API_Public/specimen?ids=BC-ZSM-HYM-25461-C10; catalogNumber: BC-ZSM-HYM-25461-C10 ; recordNumber: BC-ZSM-HYM-25461-C10; recordedBy: P. Benander ; individualID: BC-ZSM-HYM-25461-C10; individualCount: 1 ; lifeStage: adult ; Taxon: scientificName: Tetrastichusargei; phylum: Arthropoda; class: Insecta; order: Hymenoptera; family: Eulophidae; genus: Tetrastichinae; Location: country: Sweden ; decimalLatitude: 56.294 ; decimalLongitude: 12.495 ; Record Level: type: PhysicalObject; language: en; basisOfRecord: PreservedSpecimen Type status: Paratype . Occurrence: occurrenceDetails: http://www.boldsystems.org/index.php/API_Public/specimen?ids=BC-ZSM-HYM-25461-C11; catalogNumber: BC-ZSM-HYM-25461-C11 ; recordNumber: BC-ZSM-HYM-25461-C11; recordedBy: P. Benander ; individualID: BC-ZSM-HYM-25461-C11; individualCount: 1 ; lifeStage: adult ; Taxon: scientificName: Tetrastichusargei; phylum: Arthropoda; class: Insecta; order: Hymenoptera; family: Eulophidae; genus: Tetrastichinae; Location: country: Sweden ; decimalLatitude: 56.294 ; decimalLongitude: 12.495 ; Record Level: type: PhysicalObject; language: en; basisOfRecord: PreservedSpecimen Type status: Paratype . Occurrence: occurrenceDetails: http://www.boldsystems.org/index.php/API_Public/specimen?ids=BC-ZSM-HYM-25461-C12; catalogNumber: BC-ZSM-HYM-25461-C12 ; recordNumber: BC-ZSM-HYM-25461-C12; recordedBy: P. Benander ; individualID: BC-ZSM-HYM-25461-C12; individualCount: 1 ; lifeStage: adult ; Taxon: scientificName: Tetrastichusargei; phylum: Arthropoda; class: Insecta; order: Hymenoptera; family: Eulophidae; genus: Tetrastichinae; Location: country: Sweden ; decimalLatitude: 56.294 ; decimalLongitude: 12.495 ; Record Level: type: PhysicalObject; language: en; basisOfRecord: PreservedSpecimen Type status: Paratype . Occurrence: occurrenceDetails: http://www.boldsystems.org/index.php/API_Public/specimen?ids=BC-ZSM-HYM-25461-D01; catalogNumber: BC-ZSM-HYM-25461-D01 ; recordNumber: BC-ZSM-HYM-25461-D01; recordedBy: P. Benander ; individualID: BC-ZSM-HYM-25461-D01; individualCount: 1 ; lifeStage: adult ; Taxon: scientificName: Tetrastichusargei; phylum: Arthropoda; class: Insecta; order: Hymenoptera; family: Eulophidae; genus: Tetrastichinae; Location: country: Sweden ; decimalLatitude: 56.294 ; decimalLongitude: 12.495 ; Record Level: type: PhysicalObject; language: en; basisOfRecord: PreservedSpecimen Type status: Paratype . Occurrence: occurrenceDetails: http://www.boldsystems.org/index.php/API_Public/specimen?ids=BC-ZSM-HYM-25461-D02; catalogNumber: BC-ZSM-HYM-25461-D02 ; recordNumber: BC-ZSM-HYM-25461-D02; recordedBy: P. Benander ; individualID: BC-ZSM-HYM-25461-D02; individualCount: 1 ; lifeStage: adult ; Taxon: scientificName: Tetrastichusargei; phylum: Arthropoda; class: Insecta; order: Hymenoptera; family: Eulophidae; genus: Tetrastichinae; Location: country: Sweden ; decimalLatitude: 56.294 ; decimalLongitude: 12.495 ; Record Level: type: PhysicalObject; language: en; basisOfRecord: PreservedSpecimen Type status: Paratype . Occurrence: occurrenceDetails: http://www.boldsystems.org/index.php/API_Public/specimen?ids=BC-ZSM-HYM-25461-D03; catalogNumber: BC-ZSM-HYM-25461-D03 ; recordNumber: BC-ZSM-HYM-25461-D03; recordedBy: P. Benander ; individualID: BC-ZSM-HYM-25461-D03; individualCount: 1 ; lifeStage: adult ; Taxon: scientificName: Tetrastichusargei; phylum: Arthropoda; class: Insecta; order: Hymenoptera; family: Eulophidae; genus: Tetrastichinae; Location: country: Sweden ; decimalLatitude: 56.294 ; decimalLongitude: 12.495 ; Record Level: type: PhysicalObject; language: en; basisOfRecord: PreservedSpecimen Type status: Paratype . Occurrence: occurrenceDetails: http://www.boldsystems.org/index.php/API_Public/specimen?ids=BC-ZSM-HYM-25461-D04; catalogNumber: BC-ZSM-HYM-25461-D04 ; recordNumber: BC-ZSM-HYM-25461-D04; recordedBy: P. Benander ; individualID: BC-ZSM-HYM-25461-D04; individualCount: 1 ; lifeStage: adult ; Taxon: scientificName: Tetrastichusargei; phylum: Arthropoda; class: Insecta; order: Hymenoptera; family: Eulophidae; genus: Tetrastichinae; Location: country: Sweden ; decimalLatitude: 56.294 ; decimalLongitude: 12.495 ; Record Level: type: PhysicalObject; language: en; basisOfRecord: PreservedSpecimen Type status: Paratype . Occurrence: occurrenceDetails: http://www.boldsystems.org/index.php/API_Public/specimen?ids=BC-ZSM-HYM-25461-D05; catalogNumber: BC-ZSM-HYM-25461-D05 ; recordNumber: BC-ZSM-HYM-25461-D05; recordedBy: P. Benander ; individualID: BC-ZSM-HYM-25461-D05; individualCount: 1 ; lifeStage: adult ; Taxon: scientificName: Tetrastichusargei; phylum: Arthropoda; class: Insecta; order: Hymenoptera; family: Eulophidae; genus: Tetrastichinae; Location: country: Sweden ; decimalLatitude: 56.294 ; decimalLongitude: 12.495 ; Record Level: type: PhysicalObject; language: en; basisOfRecord: PreservedSpecimen Type status: Paratype . Occurrence: occurrenceDetails: http://www.boldsystems.org/index.php/API_Public/specimen?ids=BC-ZSM-HYM-25461-D06; catalogNumber: BC-ZSM-HYM-25461-D06 ; recordNumber: BC-ZSM-HYM-25461-D06; recordedBy: P. Benander ; individualID: BC-ZSM-HYM-25461-D06; individualCount: 1 ; lifeStage: adult ; Taxon: scientificName: Tetrastichusargei; phylum: Arthropoda; class: Insecta; order: Hymenoptera; family: Eulophidae; genus: Tetrastichinae; Location: country: Sweden ; decimalLatitude: 56.294 ; decimalLongitude: 12.495 ; Record Level: type: PhysicalObject; language: en; basisOfRecord: PreservedSpecimen Type status: Paratype . Occurrence: occurrenceDetails: http://www.boldsystems.org/index.php/API_Public/specimen?ids=BC-ZSM-HYM-25461-D07; catalogNumber: BC-ZSM-HYM-25461-D07 ; recordNumber: BC-ZSM-HYM-25461-D07; recordedBy: P. Benander ; individualID: BC-ZSM-HYM-25461-D07; individualCount: 1 ; lifeStage: adult ; Taxon: scientificName: Tetrastichusargei; phylum: Arthropoda; class: Insecta; order: Hymenoptera; family: Eulophidae; genus: Tetrastichinae; Location: country: Sweden ; decimalLatitude: 56.294 ; decimalLongitude: 12.495 ; Record Level: type: PhysicalObject; language: en; basisOfRecord: PreservedSpecimen Type status: Paratype . Occurrence: occurrenceDetails: http://www.boldsystems.org/index.php/API_Public/specimen?ids=BC-ZSM-HYM-25461-D08; catalogNumber: BC-ZSM-HYM-25461-D08 ; recordNumber: BC-ZSM-HYM-25461-D08; recordedBy: P. Benander ; individualID: BC-ZSM-HYM-25461-D08; individualCount: 1 ; lifeStage: adult ; Taxon: scientificName: Tetrastichusargei; phylum: Arthropoda; class: Insecta; order: Hymenoptera; family: Eulophidae; genus: Tetrastichinae; Location: country: Sweden ; decimalLatitude: 56.294 ; decimalLongitude: 12.495 ; Record Level: type: PhysicalObject; language: en; basisOfRecord: PreservedSpecimen Description FEMALE holotype (Fig. 15 ). Body length 1.8 mm (paratypes 1.5-1.9 mm). Head . Width/length in dorsal view 2.2, width/length in frontal view 1.3, POL/OOL 1.8, widths head/mesosoma 1.1, mouth width/malar space 0.9, malar space/eye height 0.8. Antenna . Scape length/eye height 1.0, pedicel+flagellum length/mesosoma width 1.3, length/width F1, F2, F3 2.0, 2.0, 1.6, clava length/width 3.4, lengths pedicel/F1 0.7, lengths F1/F2 1.0, F1/F3 1.1, lengths F1, F2, F3/clava 0.5, 0.5, 0.5, widths F1/pedicel (dorsal view) 1.1, lengths antennal spicule/C3 0.2. Mesosoma . Length/width 1.5, mesoscutal mid-lobe length/width 1.0 (width measured in anterior part), mid-lobe with median groove in posterior 3/4 , with four adnotaular setae on each side, lengths mesoscutum/mesoscutellum (measured medially) 1.3, mesoscutellum length/width 0.9, length/width of enclosed space between submedian grooves 2.7, distance between SMG/distance between SMG and SLG 1.4, lengths dorsellum/propodeum (measured medially) 0.5, propodeum with strong reticulation, propodeal callus with six setae. Fore wing . Costal cell length/width 12.8, lengths costal cell/marginal vein 1.0, lengths marginal/stigmal veins 2.9. Gaster . Subcircular to ovate, length/width 1.5, lengths gaster/mesosoma 1.2, Gt7 length/width 0.3, length of longest cercal seta/next longest seta 1.4, longest cercal seta straight, ovipositor sheaths not reaching apex of Gt7. Colour. Body metallic blue-green, entire antenna dark brown, tegulae black with metallic tinge, wings hyaline with veins yellowish-brown, coxae and femora concolorous with body, trochanters dark brown, tibiae yellowish-brown, fore tarsus brown, mid and hind tarsi yellowish-brown with T3-4 brown. MALE. Body length 1.7-1.8 mm. Head . Width/length in dorsal view 2.3, width/length in frontal view 1.2, eye height/malar space 1.2, mouth width/malar space 1.1, widths head/mesosoma 1.0. Antenna . F1-F4 without basal whorls of setae, scape length/eye height 1.1, scape length/width 2.4, ventral plaque placed in central part of scape, lengths ventral plaque/scape 0.7, pedicel+flagellum length/mesosoma width 1.5, length/width F1, F2, F3, F4 1.7, 2.0, 2.0, 2.2, clava length/width 4.4, lengths pedicel/F1 1.0, lengths F1/F2 0.8, F1/F3 0.8, F1/F4 0.7, lengths F1, F2, F3, F4/clava 0.3, 0.5, 0.5, 0.5. Colour. As in female. Diagnosis Tibiae yellowish-brown; female with malar space 0.8 x eye height, scape 3.5 x as long as wide, antennal clava 3.4 x as long as wide; mesoscutellum with ratio distance between submedian grooves to distance between submedian and sublateral grooves 1.4. Distribution Sweden. Notes Holotype deposited in MZLU, paratypes in MZLU, NHM, ZSM. Host Gregarious endoparasitoid on Arge ustulata (L.) ( Hymenoptera : Argidae ), 14♀ and 3♂ have been reared from the same host specimen. The label information does not specify which stage of the sawfly that was parasitised. Another Tetrastichus species, T. hylotomarum , has been recorded from larvae and pupae of Arge spp. (Graham 1991), but not specifically A. ustulata . Additional paratypes (not barcoded) 1♀ 1♂ "SWEDEN, Skane , Kullaberg, 28.ii.1966, P. Benander, ex Arge ustulata ".