Morphological description and DNA barcodes of adult males of Tanytarsus heliomesonyctios Langton, 1999 (Diptera, Chironomidae) in northeast of Russia Author Orel, Oksana V. Author Semenchenko, Alexander A. text Zootaxa 2019 2019-10-14 4686 1 119 126 journal article 25244 10.11646/zootaxa.4686.1.6 3d502879-6e8a-45d7-b59b-f34d64214a5f 1175-5326 3484391 1B726DDF-8EE8-4339-85C7-998F68C49266 Tanytarsus heliomesonyctios Langton, 1999 Figures 1–4 . Tanytarsus sp. Langton, 1992 (ecology, parthenogenesis, phenology). Tanytarsus heliomesonyctios Langton, 1999: 212 , Fig. 1 b, d, f (female); Fig. 2 b, e, f (pupa). Tanytarsus heliomesonyctios Langton, 1999 ; GU 073186 – GU 073192 | Female, pupa, larva, Stur & Ekrem 2011: 32 , Figs 27– 32. Tanytarsus heliomesonyctios Langton, 1999 ; AAC2863|Male, available from: http://www.boldsystems.org/index.php/Public_ RecordView?processid=CHRFI417-11 Tanytarsus heliomesonyctios Langton, 1999 ; Makarchenko et al ., 2019 , Figs 10–13. Material examined. 4 males , Russia , Magadan region , Monomtai Lake , 27–28.vii.2018 , leg. Е. Hamenkova ; 4 males , same data, 2.viii.2018 , leg. Е. Hamenkova ; 4 males , Republic of Sakha (Yakutia), Big Darpir Lake , 4.viii.2018 , leg. Е. Hamenkova. Description . Adult male (n=3). Total length 3.6–4.7 mm ; wing length 2.8–3.7 mm . Total length / wing length 1.17–1.61. Colouration . Ground colour of thorax, scutellum, maxillary palpomeres, haltere, legs and abdomen brown; antenna, scutal stripes and postnotum dark brown. Head . Frontal tubercles cone-shaped, 10–24 μm long and 7–17 μm wide. Temporal setae 16. Clypeus with 16–20 setae. Antenna with 13 flagellomeres, 1638–1701 μm long; ultimate flagellomere 1050–1071 μm. AR 1.67– 1.79. Maxillary palpomeres 2–4 combined 752–928 μm long, their individual lengths (in μm): 80–96: 216–248: 200–240: 256–344. Antenna length/palp length 1.81–2.18. Thorax . Acrostichals 4–10, dorsocentrals 8–14, prealars 2. Scutellum with 18 setae. Wing width 1.0– 1.05 mm . VR 0.94–1.18. R with 23–29, R 1 with 3–23, R 2+3 with 10–30, R 4+5 with 45–65, M 3+4 with 26–50, Cu 1 with 9 setae. VR 0.94–1.18. Brachiolum with 1 seta. Membrane covered with sparse macrotrichia in distal half. Legs (see Table 1 ). Spur of fore tibia straight, slightly curved apically 24–31 μm long. Combs of mid and hind tibiae separated; each comb bears straight or slightly curved spur, 27–31 μm (mid tibia) to 31–41 μm long (hind tibia). Basitarsus of mid leg with 2–5 sensilla chaetica. TABLE 1 . Lengths (in µm) and proportion of legs Tanytarsus heliomesonyctios Langton P f t ta1 ta2 ta3 ta4 ta5

P1	1218–1596	819–1009 1365–1722	798–882	588–735 378–504	179–221
P2	1176–1407	1113–1281 546–609	399–462	315–336 189–231	126–168
P3	1470–1827	1428–1785 924–1176	651–777	525–630 315–399	168–210
P	LR	SV	BV	BR
P1	1.65–1.71	1.49–1.51	1.81–1.93	6.36–6.75
P2	0.48–0.49	4.19–4.41	2.75–2.76	6.25–6.67
P3	0.63–0.66	3.07–3.20	2.30–2.38	8.00–9.00

Hypopygium ( Figs 1–4 ). Anal tergite with 2–3 median setae (rarely without setae) and great microtrichia-free area surrounding of base. Dark tergite bands separated medially, not reaching anal point crests. Lateral teeth and lateral setae absent. Anterior margin of tergite IX with small protrusions. The anal point is triangular shape with a wide base (85–102 μm long) and a narrower apical part (17 μm), armed with 10–12 spinulae placed in row between crests (102–133 μm long and height 7–10 μm in lateral view); microtrichia absent between crests; 16 lateral setae on each side of anal point. Gonocoxite 231–238 μm long, along the inner margin with 3–4 setae. The width of the transverse sternapodeme 119–126 µm . Phallapodeme 187–221 μm long. Superior volsella oval-shaped (65–85 μm long, 41–44 μm wide in dorsal view and 31 μm wide in lateral view), bearing 3–4 strong anteromedian and 6–8 fine dorsal setae, microtrichia absent. Digitus short (14 μm long and 7 μm wide). Stem of median volsella 78–92 μm long, bearing long simple and foliate setae that extend beyond the top of the inferior volsella. Inferior volsella 102–153 μm long, with 21–24 setae. Gonostylus straight 255–262 μm long and expanded in the proximal third (71–85 μm), tapering to widely rounded apex. HR 0.91. FIGURES 1–4. Details of the structure of hypopygium the male T. heliomesonyctios Langton. 1—total view of hypopygium, dorsal view; 2, 3—volsellae of gonocoxite; 4—superior volsella, lateral view. Scale bar 50 μm. Remarks. Adult males of T. heliomesonyctios keys to the T. lugens species group due to the missing or poorly developed digitus, usually the lack of median tergite setae, a large microtrichia free area around the anal point base, and foliate lamellae with long tips on the median volsella (Ekrem 2003). Currently, the T. lugens species group includes six species, namely, T. bathophilus Kieffer, 1911 , T. lugens (Kieffer, 1916) , T. konishii Sasa & Kawai, 1985 , T. latiforceps Edwards & Thienemann, 1941 , T. trux Gilka & Paasivirta, 2007 and T. heliomesonyctios Langton, 1999 (Ekrem 2003, Gilka & Paasivirta 2007 ). Adult males of T. heliomesonyctios most similar with two species of T . lugens species group— T. bathophilus and T. lugens . Table 2 shows the main morphological features of males. Males of T. heliomesonyctios are distinguished by a large body size, a longer last antenna segment and maxillar palp, thoracic chetotaxy, the presence of medial setae on tergite IX (rarely medial setae are absent), the presence of poorly developed digitus and elongated median volsella (see Table 2 ). Distribution. Holarctic species. Previously known from Ellesmere Island in the Canadian high Arctic and the archipelagos Spitsbergen and Jan Mayen ( Norway ) as well as in Finnmark , northern Norway (males). For the first time noted the fauna of Russia . This is the second record of adult males.