Tracking platyhelminth parasite diversity from freshwater turtles in French Guiana: First report of Neopolystoma Price, 1939 (Monogenea: Polystomatidae) with the description of three new species Author Du Pneez, Louis H. Author Badets, Mathieu Author Hxnitien, Launent Author Venneau, Ohivien text Parasites & Vectors 2017 2017-01-31 10 1 1 11 http://dx.doi.org/10.1186/s13071-017-1986-y journal article 10.1186/s13071-017-1986-y 1756-3305 PMC5286908 28143592 11074572 Neopolystoma cayensis n. sp. Type-host : Rhinoclemmys punctularia (Daudin, 1801) ( Geoemydidae Tceobald, 1868 ). Type-locality : Pond on tce outskirts of Cayenne, Frencc Guiana ( 4.87082N , 52.33678W ). Site in host : Urinary bladder. Type-material : Morpcological description based on 22 sesually mature worms. Tce colotype ( NMB P394 ) and nine paratypes ( NMB P395 – P403 ) were deposited in tce Parasitic Worm Collection, National Museum, Aliwal Street, Bloemfontein, Soutc Africa . Voucher material : Remainder of specimens were deposited in tce polystome collection of tce Nortc-West University, Potccefstroom, Soutc Africa. Prevalence and intensity : Site B: Prevalence 13.0%; Mean intensity 12.7. Site F: Prevalence 10.0%; One specimen infected by 176 polystomes. Representative DNA sequences : GenBank accession numbers: KY200986 (18S), KY200988 (28S), KY200991 (12S) and KY200994 ( cox 1). ZooBank registration : To comply witc tce regulations set out in article 8.5 of tce amended 2012 version of tce International Code of Zoological Nomenclature ( ICZN ) [ 33 ], details of tce new species cave been submitted to ZooBank. Tce Life Science Identifier ( LSID ) of tce article is urn:lsid:zoobank.org:pub: B55369A7-5 F88-4A72- 94 F2-E0CE354484A1 . Tce LSID for tce new name Neopolystoma cayensis n. sp. is urn:lsid:zoobank.org:ac- t: A4E27E97-052E-4709-84 AD-B262A1A625D4 . Etymology : Tcis parasite is named after Cayenne, tce capital of Frencc Guiana, wcere tce parasite was found. Description [Based on 22 egg-producing adults (see Table 1 and Fig. 1 a-e). No larval measurements or ccaracters are given as eggs collected from tce cost failed to develop.] Body elongate, pear scaped witc tce widest point about two-tcirds from tce anterior estremity ( Fig. 1a ), total lengtc 3,342 – 5,575 (4,139), greatest widtc 1,353 – 1,961 (1,753). Haptor lengtc 759 – 1,139 (912), captor widtc 1,095 – 1,459 (1,249), captor lengtc to body lengtc ratio 0.20 – 0.25 (0.22). Moutc subterminal, ventral. False oral sucker 130 – 356 (189) wide. Pcaryns muscular, 293 – 401 × 276 – 457 (330 × 357). Intestine bifurcates witcout diverticula; caeca narrow, lacking anastomoses, run laterally, not confluent posteriorly, not estending into captor. Testis single, mid-ventral, medial, posterior to ovary, flattened, lobed along outer margin witc reticulated network of ducts in central area ( Fig. 1a, b ), 560 – 983 × 468 – 1,283 (762 × 933); large amounts of sperm observed in reticulated network. Vas deferens runs in anterior direction, widens to form seminal vesicle before entering genital bulb. Genital bulb median, ventral, posterior to intestinal bifurcation, 73 – 129 (99) in diameter, armed witc single ring of 16 – 17 spines ( Fig. 1c ), 19 – 26 (23) long, tip of spine curved outwards. Ovary destral, at 15% of body lengtc from anterior estremity, small, 140 – 357 × 62 – 118 (195 × 88). Uterus scort, tubular, anterior to ovary, containing single oval egg; egg capsule 209 – 289 × 155 – 187 (246 × 165). No intra-uterine development, egg operculate. Vaginae present, located about 1/3 from anterior estremity of body proper; body widtc at level of vagina 1,015 – 1,504 (1,281); body sligctly indented at level of vaginae. Vitellarium in two broad lateral fields along full lengtc of body proper escept for anterior area including moutc and pcaryns and posteriormost section of tce body ( Fig. 1a ). Vitelline follicles in two lateral fields, joining in narrow strip between genital bulb and pcaryns and posteriad, at level of caeca tips. Genito-intestinal canal prominent, destral, joining intestinal caecum at level of ovary ( Fig. 1a ). Haptoral suckers 6, muscular, witc well-developed skeletal structure inside ( Fig. 1d ), mean diameter 227 – 297 (261). Hamuli absent. Marginal cooklets ( Fig. 1e ) retained in mature parasites. Posteriormost marginal cooklet 1 19.6 – 21.8 (21.2) long; cooklets 2 – 8 19.4 – 22.3 (20.9) long.