Citizen science project reveals high diversity in Didymellaceae (Pleosporales, Dothideomycetes) Author Hou, Lingwei Author Hernandez-Restrepo, Margarita Author Groenewald, Johannes Zacharias Author Cai, Lei Author Crous, Pedro W. text MycoKeys 2020 65 49 99 http://dx.doi.org/10.3897/mycokeys.65.47704 journal article http://dx.doi.org/10.3897/mycokeys.65.47704 1314-4049-65-49 DED2BF86E6FD56179642F7119604CEB9 Paraboeremia rekkeri Hern.-Restr., L. W. Hou, L. Cai & Crous sp. nov. Figure 7 Etymology. rekkeri refers to Daan Rekker who collected the soil sample from which the ex-type strain was isolated. Typus. The Netherlands. Gelderland province, Geldermalsen, isolated from garden soil, Mar. 2017, D. Rekker ( holotype designated here CBS H-24107, living ex-type culture CBS 144955 = JW 172002). Conidiomata pycnidial, superficial, scattered or aggregated, solitary or confluent, globose or subglobose, irregularly-shaped with age, buff to brown, covered with abundant mycelial outgrowths especially when young, 150-390 x 120-320 μm ; 1-2 papillate or non-papillate ostioles; pycnidial wall pseudoparenchymatous, 3-7 layers, 17.5-37 μm thick, outer layers composed of brown, flattened polygonal cells, 10-21 μm diam. Conidiogenous cells phialidic, hyaline, smooth, globose, subglobose or ampulliform, 5-10 x 4.5-7.5 μm . Conidia ellipsoidal to oblong, thin- and smooth-walled, hyaline, aseptate, 3.5-5 x 2.5-3 μm , with 2(-3) large guttules. Conidial matrix pink. Culture characteristics. Colonies after 7 d at 25 °C, on OA reaching 75-80 mm diam, aerial mycelium floccose, saffron, vinaceous buff, pale olivaceous, margin regular; reverse concentric circles saffron, grey, olivaceous grey. On MEA reaching 55-60 mm diam, aerial mycelium floccose, margin irregular, pale olivaceous grey to whitish, orange near edge; reverse brown to dark brown, orange towards the periphery. On PDA reaching 70-75 mm diam, margin irregular, covered by felty aerial mycelium, buff, olivaceous grey towards periphery; reverse mouse, olivaceous towards periphery. NaOH spot test negative on OA. Additional specimens examined. Gelderland province, Culemborg, isolated from garden soil, Mar. 2017, H. van de Warenburg, JW 3018; Kapel-Avezaath, isolated from garden soil, Mar. 2017, A. Panneman, JW 79024; Meteren, isolated from garden soil, S. van Stuijvenberg, JW 132004; North Brabant province, Breda, isolated from garden soil, Mar. 2017, F. Wiegerinck, CBS 144949 = JW 4024; Breda, isolated from garden soil, Mar. 2017, F. Versantvoort, JW 167006; Zwanenburg, isolated from garden soil, Mar. 2017, J. Rebergen, JW 91008; North Holland province, Alkmaar, Mar. 2017, B. Verschoor, JW 13016, ibid. JW 13017 and JW13030; Utrecht province, Bilthoven, isolated from garden soil, Mar. 2017, H. Vos & S. Vos, JW 51014; Eemnes, isolated from garden soil, Mar. 2017, H.W. Vos, CBS 144950 = JW 6005; Hooglanderveen, isolated from garden soil, Mar. 2017, F. Rijpma, JW 25013; Utrecht, isolated from garden soil, R. van Zijl, JW 226002. Notes. Paraboeremia rekkeri formed a well-supported (1.0/100/96) distinct lineage in Paraboeremia (Figure 1 ). It is most closely related with P. truiniorum , another novel species collected from Dutch soil and described in the present study. However, P. rekkeri is distinguished by producing larger pycnidia (150-390 x 120-320 μm ), with a thinner pycnidial wall (3-7 layers and 17.5-37 μm thick). Pycnidia in P. truiniorum are 160-420 x 135-430 μm , and have a wall of 7-11 layers and 40-70 μm thick. Figure 7. Paraboeremia rekkeri (CBS 144955). A, B Colony on OA (front and reverse) C, D colony on MEA (front and reverse) E, F colony on PDA (front and reverse) G pycnidia forming on OA H pycnidium I section of pycnidium J section of pycnidial wall K-N conidiogenous cells O conidia. Scale bars: 100 μm ( H ); 20 μm ( I ); 10 μm ( J ); 5 μm ( K-O ).