Two new species of Cladosporium from leaf spots of Paris polyphylla in north-western Yunnan Province, China Author Xu, Yue-Xin https://orcid.org/0000-0002-9925-6721 College of Public Health, University of South China, Hengyang, China & College of Agriculture and Biological Sciences, Dali University, Dali, China Author Shen, Hong-Wei College of Agriculture and Biological Sciences, Dali University, Dali, China & Center of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai, Thailand & School of Science, Mae Fah Luang University, Chiang Rai, Thailand Author Bao, Dan-Feng College of Agriculture and Biological Sciences, Dali University, Dali, China & Center of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai, Thailand & Department of Entomology and Plant Pathology, Faculty of Agriculture, Chiang Mai University, Chiang Mai, Thailand Author Luo, Zong-Long https://orcid.org/0000-0001-7307-4885 College of Agriculture and Biological Sciences, Dali University, Dali, China Author Su, Hong-Yan College of Agriculture and Biological Sciences, Dali University, Dali, China suhongyan16@163.com Author Hao, Yu-E College of Public Health, University of South China, Hengyang, China haohao200701@126.com text Biodiversity Data Journal 2021 2021-12-23 9 77224 77224 http://dx.doi.org/10.3897/BDJ.9.e77224 journal article http://dx.doi.org/10.3897/BDJ.9.e77224 1314-2828-9-e77224 D8E6587EFB6F59889C0E266885DF499D Cladosporium yunnanensis H.W. Shen, Y.X. Xu, H.Y. Su & Z.L. Luo sp. nov. Materials Type status: Holotype . Occurrence : recordedBy: Yue-Xin Xu ; Taxon : scientificName: Cladosporium yunnanensis; kingdom: Fungi ; phylum: Ascomycota ; class: Dothideomycetes ; order: Capnodiales ; family: Cladosporiaceae ; genus: Cladosporium ; Location : locationRemarks: China , Yunnan Province , Dali , on diseased leaves of Paris polyphylla, 2 October 2020 ; Event: day: 2020; habitat: leaf spots of Paris polyphylla; Record Level: collectionID: 1CL JD 5-1-4; collectionCode: Y-23 Description Asexual morph : hyphomycetous (Fig. 2 ). Mycelium superficial and immersed, composed of septate, branched, subhyaline, smooth-walled, 1-3 μm wide. Conidiophores macronematous, 127-190 x 4-6 μm (x̄ = 158.2 x 5.1 μm , n = 15), solitary or in small loose groups, erect to slightly flexuous, non-nodulose, sometimes subnodulose at the uppermost apex, unbranched, 0-6 septate, sometimes slightly constricted at septa, pale brown, smooth, sometimes somewhat irregularly rough-walled or verruculose. Conidiogenous cells terminal and intercalary, loci crowded at the apex forming clusters of pronounced scars, 1-2 conidiogenous loci formed at about the same level, loci often situated at lateral shoulders due to sympodial proliferation, loci 1-2 μm diam. Conidia solitary or in short unbranched chains, straight to slightly curved, cylindrical-oblong, 7-19 x 5-7 μm (x̄ = 13.2 x 5.7 μm , n = 30), 0-3 septate, sometimes slightly constricted at the septa, pale to pale medium olivaceous-brown. Sexual morph : Undetermined. Culture characteristics : Colonies on PDA attaining 25 mm diam. after 7 d, 45 mm diam. after 14 d and covering the whole Petridish after 30 d, dark green to olive green, velvety, furrowed; reverse dark green to black. Material examined : China, Yunnan Province, Dali, on diseased leaves of Paris polyphylla , 2 October 2020, Y.X. Xu, Y-23. (KUN-HKAS 121704, holotype ), ex-type living culture CGMCC 3.20622 = KUNCC 21-10712 Etymology " Cladosporium yunnanensis " refers to Yunnan Province, China, where the species was collected. Distribution China, Yunnan Province, Dali, on diseased leaves of Paris polyphylla Notes Based on the multi-locus phylogenetic analysis (Fig. 1 ), Cladosporium yunnanensis grouped in a well-supported clade, together with C. cladosporioides and C. magnoliigena . However, the genetic distance allows it to be considered a distinct species within the clade (Fig. 1 ). Morphologically, C. yunnanensis has much shorter conidiophores than C. cladosporioides (up to 190 μm vs. up to 350 μm ), but longer than C. magnoliigena (up to 190 μm vs. up to 150 μm ). Moreover, the new species differs from C. cladosporioides by the smaller conidiogenous cells (7-19 x 5-7 μm vs. 4-18 x 2-5 μm ), but larger than C. magnoliigena (7-19 x 5-7 μm vs. 4-18 x 2-5 μm ) ( Bensch et al. 2012 , Jayasiri et al. 2019 ). The BLAST analysis of TEF1-α and ACT shows that C. yunnanensis (KUN-HKAS 121704) is different from C. cladosporioides (CBS 112388) by 16 and 10 nucleotide differences, respectively and the comparison of TEF1-α between C. yunnanensis (KUN-HKAS 121704) and C. magnoliigena (CBS 140463) reveals 33 nucleotide differences.