Two new species and one new record of Parakiefferiella Thienemann, 1936 from China (Diptera, Chironomidae) Author Liu, Wenbin Author Sun, Bingjiao Author Yan, Chuncai Author Song, Chao Author Wang, Xinhua text ZooKeys 2015 545 139 148 http://dx.doi.org/10.3897/zookeys.545.6000 journal article http://dx.doi.org/10.3897/zookeys.545.6000 1313-2970-545-139 FEC231B1B08E400892C72D319E551C42 FEC231B1B08E400892C72D319E551C42 Taxon classification Animalia Diptera Chironomidae Parakiefferiella fasciata Liu & Wang sp. n. Figs 1-7 Type material. Holotype: ♂ (BDN. No.1165), China, Shandong Province, Yantai City, Kunyu Mountain, 37°30'10"N , 121°23'40"E , 24.viii.1987, sweeping net, Wang XH. The specimens were sealed with Canada balsam on slides. Paratypes: 3 ♂♂, data as holotype. Diagnosis. The adult male can be distinguished from known species of the genus by the following combination of characters: anal point lacking keel, very broad at base, rounded apically, without basal setae; virga consisting of four spines; antenna with ten flagellomeres, AR 0.47-0.50; tergites III and VIII dark brown, others tergites pale yellow; HR 1.98-2.06; HV 2.65-3.00. Description. Male imago (n = 4). Total length 1.04-1.09, 1.07 mm. Wing length 0.71-0.72, 0.71 mm. Total length/wing length 1.45-1.56, 1.47. Wing length/length of profemur 3.24-3.29, 3.26. Coloration. Head brown. Thorax brown with dark spot. Tergites (Fig. 4) III and VIII dark brown, others tergites pale yellow. Figures 1-7. Parakiefferiella fasciata Liu & Wang, sp. n., male. 1 wing 2 antenna 3 thorax 4 abdomen tergites coloration 5 hypopygium (dorsal view) 6 inferior; 7 hypopygium (ventral view). Head. Antenna (Fig. 2) with ten flagellomeres. AR 0.47-0.50, 0.49. Ultimate flagellomere 137-144, 140 μm long. Inner vertical 1. Clypeus with four setae. Tentorium 55-72, 66 μm long, 5-7, 6 μm wide. Palpomere lengths (in μm ): 10-12, 11; 14-19, 16; 26-29, 28; 43-48, 47; 88-96, 92. L: 5th/3rd: 3.26-3.55, 3.43 Wing (Fig. 1). Anal lobe reduced. VR 1.12-1.21, 1.17. Costal extension 40 μm long. Brachiolum with one seta. All veins bare. Squama bare. Thorax (Fig. 3). Antepronotal lobes developed. Antepronotum with one lateral seta. Dorsocentrals 2-4, 3; one prealars. Scutellum with 2-6, 5 setae. Mesonotum with a tuft of hairs. Legs. Spur of fore tibia 24-25, 24 μm long; spurs of mid tibia 9-12, 11 μm and 12-14, 13 μm long; spurs of hind tibia 9-10, 9 μm and 20-22, 21 μm long. Hind tibial comb with 9-12, 11 spines, 19-38, 30 μm long. Width at apex of fore tibia 17-19, 19 mm, of mid tibia 19-20, 19 mm, of hind tibia 21-24, 23 mm. Lengths (in μm ) and proportions of legs as in Table 1. Table 1. Lengths (in μm ) and proportions of legs of Parakiefferiella fasciata Liu & Wang, sp. n. (n = 4)

P 1	P 2	P 3
1
2
3
4
5

Hypopygium (Figs 5-7). Anal point triangular and hyaline, very broad at base, with rounded apex and without basal seta. Laterosernite IX with 2-4, 3 setae. Phallapodeme 20-24, 23 μm long. Transverse sternapodeme 48-54, 53 μm long. Virga consisting of four spines, 20 -35, 25 μm long. Tergites III and VIII bearing colored bands. Gonocoxite 72-74, 73 μm long, outer margins concave medially. Superior volsella absent; inferior volsella rounded and very large, occupying nearly half of the total length of gonocoxite, lacking dorsal setae and densely covered with microtrichia (Fig. 6); ventral margin strongly swollen medially. Gonostylus 36-38, 37 μm long, anterior margin nearly straight, posterior margin slightly curved in dorsal view. Megaseta 5-7, 7 μm long. Crista dorsalis reduced, slightly visible in lateral view. HR 1.98-2.06, 2.01. HV 2.65-3.00, 2.75. Etymology. The specific name is from Latin fasciata, referring to different colored bands in its tergites. Remarks. The new described species is distinguished from all other Parakiefferiella species by the following unusual combination of characters: antenna with ten flagellomeres; tergites III and VIII with colored bands; anal point lacking both dorsal setae and keel; absence of dorsal setae on inferior volsella, which is bearing only microtrichia. While the new species close related to Parakiefferiella coronata (Edwards, 1929; Makarchenko and Makarchenko 2010 ). However, the new species differs from the latter on the basis of following characters: (1) the antenna of the new species with ten flagellomeres, whereas Parakiefferiella coronata has 13 flagellomeres; (2) the superior volsella of Parakiefferiella coronata is large, whereas in the new species it is absent; (3) the new species has abdomen pale yellow with tergites III-VIII dark brown, whereas Parakiefferiella coronata has abdomen dark with tergites IV, VI-VII shiny. Female and immature stages unknown.