DNA barcoding of the Italian anecic Octodrilus species in rural (vineyard) and forested areas with description of Octodrilus zicsiniello sp. nov. (Clitellata, Megadrili) Author Csuzdi, Csaba Author Szederjesi, Tímea Author Marchán, Daniel Fernández Author Sosa, Irene De Author Gavinelli, Federico Author Dorigo, Luca Author Pamio, Alberto Author Dreon, Angelo Leandro Author Fusaro, Silvia Author Moretto, Enzo Author Paoletti, Maurizio Guido text Zootaxa 2018 2018-10-05 4496 1 43 64 journal article 29240 10.11646/zootaxa.4496.1.5 1c5e0132-a83c-400e-bd2c-23cf881b28ff 1175-5326 1447039 F658011C-415A-464F-B947-E2DF422005AC Octodrilus istrianus Mršić, 1991 Octodrilus istrianus MršIć 1991 Monogr. EArThw. BALkAns: 406. Octodrilus istrianus : HAckEnbErgEr & HAckEnbErgEr 2013 ZooTAXA, 3710: 19. Material examined . Brioni 2 ( EWITA196-15 ) : Brijuni Island , Croatia , 30/11/2014 , leg. Enzo Moretto . Torrente V1 ( MH 032883 ) , V3 ( MH 032884 ) , V4 ( MH 032885 ), V5 , V6 : Zlepcari , Pican , Croatia , 15/05/2016 , leg. Maurizio G. Paoletti & Enzo Moretto. ( Altogether six specimens). Diagnosis . Large sized worms, 250–300 mm by 10–15 mm . Colour grey on dorsum and paler on ventrum. Male pore on 15 small. Clitellum on 1/n 29, 29–42, 1/n 43; tubercles on 29, 30–42, 1/n 43. Nephridiopores irregularly alternate between b and above d . Two pairs of testes enclosed in perioesophageal testis sacs in 10, 11. Vesicles in 9–12, those in 11 sometimes smaller. Spermathecae seven pairs, in 6/7–12/13 . Remarks . Oc. istrianus has relatively recently been described and has not been reported after the original description until now. Our specimens, collected also on the Istria peninsula, completely agree with the original description. Morphologically, it stands in between Oc. mimus and Oc. tergestinus (cl. 28, 29–40 and 28, 29–44 respectively) however, on our molecular tree these specimens form an unresolved, well-supported subclade (0.96 PP) inside the tergestinus clade. The status of this species needs further clarification by analysing more material from or near the type locality and involving more genes.