Additions and corrections to taxonomy of Apobaetis fiuzai Salles & Lugo-Ortiz, 2002 and Apobaetis kallawaya Nieto, 2006 (Ephemeroptera, Baetidae) Author De Lima, Cláudia R. T. 18795D2E-427D-4A26-B112-70C929CA5B95 Instituto Nacional de Pesquisas da Amazônia (INPA), Coordenação de Pós-Graduação (COPOG), Divisão do Curso em Entomologia (DiEnt), Coordenação de Biodiversidade (CoBio), Laboratório de Citotaxonomia e Insetos Aquáticos, CEP 69067 - 375, Manaus, Amazonas, Brazil. Universidade Federal de Rondônia (UNIR), Departamento de Biologia, Laboratório de Biodiversidade e Conservação, 76.801 - 059, Porto Velho, Rondônia, Brazil. claudia.tavares.lab@gmail.com Author Cruz, Paulo Vilela 34F85F41-2743-4248-813F-0CEEE63FE649 Instituto Nacional de Pesquisas da Amazônia (INPA), Coordenação de Pós-Graduação (COPOG), Divisão do Curso em Entomologia (DiEnt), Coordenação de Biodiversidade (CoBio), Laboratório de Citotaxonomia e Insetos Aquáticos, CEP 69067 - 375, Manaus, Amazonas, Brazil. Universidade Federal de Rondônia (UNIR), Departamento de Biologia, Laboratório de Biodiversidade e Conservação, 76.801 - 059, Porto Velho, Rondônia, Brazil. pvilelacruz@gmail.com Author Hamada, Neusa 0D02F36C-6B09-423C-95A6-6179E9808CD5 Instituto Nacional de Pesquisas da Amazônia (INPA), Coordenação de Pós-Graduação (COPOG), Divisão do Curso em Entomologia (DiEnt), Coordenação de Biodiversidade (CoBio), Laboratório de Citotaxonomia e Insetos Aquáticos, CEP 69067 - 375, Manaus, Amazonas, Brazil. Universidade Federal de Rondônia (UNIR), Departamento de Biologia, Laboratório de Biodiversidade e Conservação, 76.801 - 059, Porto Velho, Rondônia, Brazil. neusaha@gmail.com text European Journal of Taxonomy 2023 2023-07-13 879 1 136 161 http://dx.doi.org/10.5852/ejt.2023.879.2167 journal article 55107 10.5852/ejt.2023.879.2167 ce30301f-248e-4674-bd0c-fcfad4f875ee 2118-9773 8155397 A49B65B5-F27A-44EC-9A13-A39BF72F6E61 Apobaetis jaquelinae sp. nov. urn:lsid:zoobank.org:act: 806D9CCD-9AB3-402E-9D58-FA64C2C1DEF4 Figs 1 , 6–8 Apobaetis fiuzai – Boldrini & Cruz 2014: 4 . Diagnosis NYMPH . Characterized by a combination of the following characters: 1) labrum rectangular, distal medial margin with one protuberance; dorsal surface with 3 elongated and blunt medial setae near distal margin ( Fig. 7A–B ); 2) hypopharynx with lingua subcircular, with apical tuft of setae, length subequal to superlingua ( Fig. 7E ); 3) maxillary palp long 1.7× length of galea-lacinia; segment II without apical constriction ( Fig. 7F ); 4) labial palp with segment II with triangular distomedial projection with rounded apex, laterally directed; segment III rectangular, distal margin concave ( Fig. 7G ); 5) foreleg with anterior surface of femur with one row of 8 to 9 short concave setae slightly pectinated on apex; apex with 2 short concave setae slightly pectinated on apex; claw 0.6–0.7 × length of tarsus, with two row of denticles restricted to middle portion ( Fig. 8A–C ); 6) posterior margin of tergum IV with triangular and pointed spines in regular sizes ( Fig. 8D ); 7) paraproct with several marginal spines, posterolateral extension with blunt spines ( Fig. 8F ). Etymology The species name is a tribute to Dr Jaqueline Góes, from the Instituto de Medicina Tropical de São Paulo , who was part of the team that developed and improved the viral genome sequencing protocols for the rapid sequencing of the coronavirus (SARS-CoV2). Fig. 6. Apobaetis jaquelinae sp. nov. , habitus of nymph. A–B . Holotype (INPA). A . Nymph male (d.v.). B . Nymph male (v.v.). C . Paratype (INPA), Nymph female (d.v.). Abbreviations: d.v. = dorsal view; v.v. = ventral view. Not to scale. Fig. 7. Apobaetis jaquelinae sp. nov. , holotype (INPA), mouthparts of nymph. A . Labrum (left d.v., right v.v). B . Detail of medial spatulate setae on dorsal surface of labrum. C . Left mandible. D . Right mandible. E . Hypopharynx. F . Maxilla. G . labium (left d.v., right v.v.). Abbreviations: d.v. = dorsal view; v.v. = ventral view. Not to scale. Material examined Holotype BRAZIL • nymph on slide; Rondônia , Teixeirópolis , Vale das Cachoeiras ; 10º55′20.4″ S , 62º22′34.7″ W ; 10 Jul. 2018 ; P. V . Cruz, N . Hamada and G. Desidério leg.; INPA . Paratypes BRAZIL • 2 nymphs on slide; same collection data as for holotype; INPA . Additional material BRAZIL – Rondonia • 1 nymph on slide; Colorado do Oeste , Rio Cabixi ; 13º15′31.8″ S , 60º20′04.8″ W ; 3 Sep. 2012 ; N. Hamada and R . Boldrini leg.; INPA • 1 nymph on slide; Nova Londrina , Rio Urupá ; 11º02′05.8″ S , 62º08′34.1″ W ; 9 Jul. 2018 ; P. V . Cruz, N . Hamada and G. Desidério leg.; INPA . Description Nymph LENGTH . Body: 2.5–2.8 mm . HEAD . Antenna: flagellum with minute spines on apex of each flagellomere. Labrum ( Fig. 7A–B ): length about 0.6× of maximum width; distal medial margin with one protuberance, distolateral margin rounded; dorsal surface with 3 elongated and blunt medial setae near distal margin; medially with one row of long and thin setae near distal margin; long, thin and simple setae covering dorsal surface; ventral surface with one row of spine-like setae on distolateral and distal margins. Left mandible ( Fig. 7C ): incisors not fused; outer and inner set of incisors with 4 and 3 denticles, respectively; prostheca robust, bifurcated at apex, outer lobe robust, inner lobe slender with medial tuft of setae; margin between prostheca and mola concave; subtriangular process wide with short protuberance on distal margin; tuft of setae at base of subtriangular process; denticles of mola not constricted; lateral margin convex. Right mandible ( Fig. 7D ): incisors not fused; outer and inner set of incisors each with 3 denticles; prostheca slender, bifurcated at apex, tuft medial of minute setae; margin between prostheca and mola concave; tuft of setae at base of mola absent; denticles of mola not constricted; lateral margin convex. Hypopharynx ( Fig. 7E ): lingua subcircular, apex covered with short setae, subequal to superlingua; superlingua not expanded, with short, thin, simple setae over distolateral and distal margin. Maxilla ( Fig. 7F ): maxillary palp long, 1.7× length of galea-lacinia; segment I 0.6× length of galea-lacinia; segment II tapering slightly from base to apex, with thin and simple setae scattered on surface, without apical constriction; medial margin of galea-lacinia with 2 to 3 spine-like setae. Labium ( Fig. 7G ): glossa subcircular, robust, narrowing apically, subequal to paraglossa; dorsal surface with one longitudinal row of 4 short spine-like setae near inner margin; 2 short spine-like setae near internal margin, thin and simple setae and one robust spine-like setae on apex; longitudinal row of 9 robust spine-like setae on apical ⅔ near outer margin; ventral surface covered with long, thin and simple setae. Paraglossa curved inward; dorsal surface with long and robust spine-like setae on apex; longitudinal row of 15 long spine-like setae on apical ⅔ near outer margin and one longitudinal row of 5 long and robust spine-like setae on apical ⅔ near inner margin; ventral surface with one longitudinal row of 5 robust and long spine-like setae on apical ⅔. Labial palp with segment I 0.7 × length of segments II and III combined, covered with micropores (not illustrated); segment II with outer margin covered with thin, long and simple setae, inner margin bare; segment II with triangular distomedial projection, with rounded apex, laterally directed; ventral surface of distomedial projection with thin, long and simple tuft of setae; segment III rectangular, with concave distal margin, length 0.6× width, covered with thin, long and simple setae on outer margin, dorsally with one row of 11 to 13 spine-like setae in different sizes near distal margin, ventrally with 8 to 10 spine-like setae in different sizes near distal margin. Fig. 8. Apobaetis jaquelinae sp. nov. , holotype (INPA), thorax and abdomen of nymph. A . Foreleg (femur on anterior surface). B . Detail of setae on anterior surface of forefemur. C . Claw. D . Posterior margin of tergum IV. E . Gill. F . Paraproct. G . Cercus. H . paracercus. Not to scale. THORAX . Holotype pigment ( Fig. 6A–B ): light yellow, with brown marks; forewing pads with basal brown mark. Paratype pigment, nymph female ( Fig. 6C ): light yellow, with lateral brown mark. Foreleg ( Fig. 8A–C ). Femur: anterior surface with one row with 8 to 9 short concave setae slightly pectinated on apex near dorsal margin; apex of femur with 2 short concave setae slightly pectinated on apex; ventral margin with one row of 10 to 12 spine-like setae of different sizes. Tibia: dorsal margin bare; ventral margin with one row of 10 to 11 spine-like setae. Patella-tibial suture present, from dorsal margin to ventral margin. Tarsus: dorsal margin bare; ventral margin with one row of 11 to 12 spine-like setae. Claw: 0.6–0.7× length of tarsus, with two rows of denticles restricted to middle portion. Middle and hindleg similar to foreleg. ABDOMEN . Holotype pigment, nymph male ( Fig. 6A–B ): tergum V with lateral brown mark; posterior margin of tergum V with medial brown mark and sublateral brown spots; tergum VI with central brown mark; anterior margin of tergum VII with medial brown mark and short spots; terga VIII and IX with lateral brown mark and short spots; sterna II–VII with anterolateral brown mark and with lateral brown mark; sternum VIII brown; sternum IX with anterior margin brown mark. Paratype pigment. Nymph female ( Fig. 6C ). Tergum II with medial brown mark; terga III and V with lateral brown mark; terga II– IV covered by reddish brown pigmentation. Tergum IV ( Fig. 8D ): surface covered by triangular scale-like projections and micropores; posterior margin with triangular and pointed spines, in regular sizes. Gill ( Fig. 8E ): triangular apex, trachea conspicuous; long length, extending to half of third subsequent tergum. Paraproct ( Fig. 8F ) with several marginal spines. Posterolateral extension with blunt spines. Cerci ( Fig. 8G ) with spines in all segments. Paracercus ( Fig. 8H ) with prominent spines in all segments.