Unexpected high species diversity among European stalked puffballs - a contribution to the phylogeny and taxonomy of the genus Tulostoma (Agaricales) Author Jeppson, Mikael Author Altes, Alberto Author Moreno, Gabriel Author Nilsson, R. Henrik Author Yolanda Loarce, Author Bustos, Alfredo de Author Larsson, Ellen text MycoKeys 2017 21 33 88 http://dx.doi.org/10.3897/mycokeys.21.12176 journal article http://dx.doi.org/10.3897/mycokeys.21.12176 1314-4049--33 Tulostoma lusitanicum Calonge & M.G. Almeida, in Calonge, Bol. Soc. Micol. Madrid 25: 302. 2000. Figure 12 Holotype. PORTUGAL, Estremadura: Pinhal do Rei, 8 Jan. 1991, G. Almeida, F. Estrella, F.D. Calonge (LISU-MGA 8!)*. The species was invalidly published by Calonge and Almeida (1992) and later validated by Calonge (2000) . ITS sequence data confirm it as a distinct species (Figure 1b). Habitat and distribution. Recorded only from sand dunes in Portugal. Figure 12. T. lusitanicum , holotype (LISU-MGA 8): a basidiocarp b spore ornamentation under SEM. Scale bars: a = 10 mm; b = 1 µm .