Seven soil-inhabiting new species of the genus Trichoderma in the Viride clade
Author
Chen, Kai
State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China & University of Chinese Academy of Sciences, Beijing 100049, China
Author
Zhuang, Wen-Ying
State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China & University of Chinese Academy of Sciences, Beijing 100049, China
text
Phytotaxa
2017
2017-07-04
312
1
28
46
http://dx.doi.org/10.11646/phytotaxa.312.1.2
journal article
10.11646/phytotaxa.312.1.2
1179-3163
13701723
Trichoderma adaptatum
K. Chen & W.Y. Zhuang
,
sp. nov.
Fig 2
Fungal Names: FN570314.
Etymology
: The specific epithet refers to adaption of diverse living conditions.
Diagnosis
. Colony fast-growing on CMD and PDA, slow-growing on SNA at 25 °C. Conidiophores trichoderma-like, phialides narrowly lageniform, conidia green, subglobose to ellipsoid.
On CMD after 72 h colony radius
65–66 mm
and mycelium covering the plate after 3 d at 25 °C. Colony hyaline, radial, margin well defined, aerial hyphae common, more abundant with distance from the original inoculum. Conidiation noted after 2 d, formed on aerial hyphae and shrubs, shrubs mainly concentrated along the colony margin. Conidiophores symmetrical, trichoderma-like, numerous. Phialides solitary or paired or in whorls of 3–4, typically narrowly lageniform, sometimes ampulliform, (6.9–)9.0–13.8(–21.9) × 2.5–3.5 μm, l/
w 2.3
–5.0(–8.8), 1.7–2.8 μm wide at the base (n = 40). Conidia green, smooth, subglobose to ellipsoid, less commonly globose, 3.1–4.4(–5.3) × 2.8–3.6 μm, l/
w 1.0
–1.5 (n=40). No chlamydospores observed. No distinct odor, no diffusing pigment observed.
On PDA after 72 h colony radius
62–65 mm
and mycelium covering the plate after 4 d at 25 °C. Colony radial, indistinctly zonate, margin well defined. Aerial hyphae abundant, forming a downy, zonate mat. Conidiation noted after 2 d, formed on aerial hyphae and tufts. No chlamydospores observed. No distinct odor, no diffusing pigment observed.
On SNA after 72 h colony radius
13 mm
and mycelium covering the plate after 11 d at 25 °C. Colony hyaline, loose, margin well defined, aerial hyphae common. Conidiation noted after 3 d, formed in loose shrubs, first white, turning green after 4 d. No chlamydospores observed. No distinct odor, no diffusing pigment observed.
Strains examined
:
CHINA
.
Tibet
:
Linzhi
, elev.
3258 m
, isolated from soil,
21 November 2014
,
K. Chen
TC408
(
holotype
HMAS 248800
, ex-type culture
CGMCC 3.18321
).
ibid.
,
K. Chen
TC403
(
HMAS 248802
)
;
K. Chen
TC404
(
HMAS 248803
).
CHINA
.
Beijing
:
Yanqing
,
Yudushan
, elev.
926 m
, isolated from soil,
8 July 2015
,
K. Chen
TC734
(
HMAS 248801
)
;
Notes
:
Trichoderma adaptatum
was isolated from
Tibet
and
Beijing
, the areas have significantly different climates, which indicates that the fungus might be widely distributed in the county. In
Fig 1
,
T. adaptatum
appeared as a separate terminal branch in the Viride clade, and is not closely related to any other species.
Trichoderma tibetense
is also from
Tibet
(
Chen & Zhuang 2016
), but is distantly related and belongs to the Strictipile clade.