The genus Leucophenga (Diptera, Drosophilidae), part IV: the ornata species group from the East Asia, with morphological and molecular evidence (II) Author Huang, Jia Author Li, Tong Author Chen, Hongwei text Zootaxa 2014 3893 1 1 55 journal article 10.11646/zootaxa.3893.1.1 c3104971-b07a-424c-81e9-ef313376b7d2 1175-5326 287539 D0600C5C-DA06-47C5-80CD-4E625EBC6259 Leucophenga atrinervis Okada, 1968 ( Figs 2 A, 5A–D, 10A, 13A, 13B, 18) Leucophenga atrinervis Okada, 1968 : 313 ; 1990: 683. Diagnosis. Aedeagus curved subbasally and subapically, with pubescence distally, acute apically ( Fig. 18 D). Description. Ocellar triangle yellowish brown, with 4–8 setae above ocellar setae. Frons yellowish brown to brown. Pedicel mostly yellowish brown; first flagellomere yellow. Clypeus brownish medially, dark brown laterally. Palpus brownish yellow. Mesonotum brownish yellow to yellowish brown, with brown, longitudinal stripes laterally ( Fig. 5 A, B), sometimes dark brown medially ( Fig. 5 A). Postpronotal lobe yellow ( Fig. 5 C, D). Acrostichal setulae in 10–12 irregular rows ( Fig. 5 A, B). Scutellum brownish yellow to yellowish brown, dark brown laterally, yellow at tip ( Fig. 5 A, B). Pleura with a brown, longitudinal stripe above ( Fig. 5 C, D). Katepisternum and mesopleuron (meron in Huang et al . 2013a , b ) mostly brownish yellow; mesopleuron with a brownish patch ( Fig. 5 C, D). Wing ( Fig. 2 A): Costal vein between R2+3 and R4+5 distally with 5–8 peg-like spinules on ventral surface. Abdominal tergites black, with yellow patches ( Fig. 10 A). Male terminalia: Epandrium nearly entirely pubescent, with 17 setae near posterior margin and ventral corner per side ( Fig. 18 A). Hypandrium with long pubescence anteriorly ( Fig. 18 B). Paramere with 6 sensilla submediodorsally ( Fig. 18 C). FIGURE 2. Wing of male: A. Leucophenga atrinervis ; B. L . digmasoma ; C. L . multipunctata ; D. L . nigrinervis ; E. L . pectinata in male; F. L . pectinata in female; G. L . pentapunctata ; H. L . quadripunctata ; I. L . regina ; J. L . trivittata . Scale bars = 1 mm. FIGURE 3. Wing of male: A. Leucophenga acutifoliacea sp. nov. ; B. L . albiterga sp. nov. ; C. L . angustifoliacea sp. nov. ; D. L . baculifoliacea sp. nov. ; E. L . cornuta sp. nov. ; F, G. L . hirticeps sp. nov. ; H. L . latifascia sp. nov. ; I. L . pinguifoliacea sp. nov. ; J. L . retihirta sp. nov. Scale bars = 1 mm. FIGURE 4. Wing of male: A. Leucophenga securis sp. nov. ; B. L . sinupenis sp. nov. ; C. L . villosa sp. nov. Scale bars = 1 mm TABLE 2. The summary of genetic distances in and between species.
Species Number of sequences Intra./Min. intra./Max. intra./Mean intra. Vari. Min. inter./Max. inter./Mean inter. Vari.
atrinervis 4 0.003/0.031/0.018±0.013 0.049/0.105/0.082±0.012
digmasoma 7 0.000/0.034/0.023±0.014 0.036/0.110/0.083±0.015
multipunctata 1 #/#/# 0.050/0.110/0.078±0.014
nigrinervis 8 0.003/0.052/0.032±0.017 0.041/0.114/0.083±0.016
pectinata 10 0.000/0.088/0.041±0.037 0.064/0.114/0.092±0.009
pentapunctata 6 0.000/0.082/0.047±0.035 0.006/0.133/0.088±0.013
quadripunctata 8 0.000/0.052/0.023±0.021 0.038/0.097/0.073±0.012
regina 3 0.000/0.038/0.025±0.022 0.064/0.111/0.093±0.010
trivittata 3 0.000/0.000/0.000±0.000 0.061/0.102/0.082±0.010
acutifoliacea 1 #/#/# 0.047/0.100/0.074±0.013
albiterga 1 #/#/# 0.075/0.114/0.093±0.009
angustifoliacea 1 #/#/# 0.047/0.100/0.072±0.012
baculifoliacea 1 #/#/# 0.075/0.111/0.095±0.009
cornuta 1 #/#/# 0.061/0.111/0.087±0.012
hirticeps 5 0.002/0.041/0.019±0.017 0.036/0.113/0.081±0.015
latifascia 9 0.000/0.066/0.038±0.025 0.050/0.111/0.079±0.011
pinguifoliacea 4 0.011/0.050/0.036±0.015 0.063/0.133/0.092±0.013
retihirta 1 #/#/# 0.038/0.097/0.071±0.014
securis 4 0.000/0.031/0.016±0.017 0.042/0.103/0.077±0.012
sinupenis 6 0.002/0.006/0.004±0.002 0.050/0.107/0.076±0.012
villosa 1 #/#/# 0.049/0.111/0.086±0.013
Total 85 0.000/0.088/0.030±0.027 0.036/0.133/0.083±0.014
Min. intra., minimum intraspecific distance; Max. intra., maximum intraspecific distance; Mean intra. Vari., mean intraspecific variability; Min. inter., minimum interspecific distance; Max. inter., maximum interspecific distance; Mean inter. Vari., mean interspecific variability. #, no applicable. . FIGURE 5. Dorsal and lateral thorax of male: A, B, C, D. Leucophenga atrinervis ; E, F, G. L . digmasoma ; H, I. L . multipunctata ; J. L . nigrinervis . FIGURE 6. Dorsal and lateral thorax of male: A, B. Leucophenga nigrinervis ; C, D. L . pectinata ; E, F, G, H. L . pentapunctata ; I, J. L . quadripunctata . FIGURE 7. Dorsal and lateral thorax of male: A, B. Leucophenga regina ; C, D. L . trivittata ; E, F. L . acutifoliacea sp. nov. ; G, H. L . albiterga sp. nov. ; I, J. L . angustifoliacea sp. nov. . FIGURE 8. Dorsal and lateral thorax of male: A, B. Leucophenga baculifoliacea sp. nov. ; C, D. L . cornuta sp. nov. ; E, F. L . hirticeps sp. nov. ; G, H, I. L . latifascia sp. nov. ; J. L . pinguifoliacea sp. nov. . FIGURE 9. Dorsal and lateral thorax of male: A, B. Leucophenga pinguifoliacea sp. nov. ; C, D. L . retihirta sp. nov. ; E, F. L . securis sp. nov. ; G, H. L . sinupenis sp. nov. ; I. J. L . villosa sp. nov. FIGURE 10. Abdominal tergite pattern in male: A. Leucophenga atrinervis ; B, C. L . digmasoma ; D. L . multipunctata ; E. L . nigrinervis ; F. L . pectinata ; G, H. L . pentapunctata ; I, J. L . quadripunctat ; K. L . regina ; L. L . trivittata . FIGURE 11. Abdominal tergite pattern in male: A. Leucophenga acutifoliacea sp. nov. ; B. L . albiterga sp. nov. ; C. L . angustifoliacea sp. nov. ; D. L . baculifoliacea sp. nov. ; E. L . cornuta sp. nov. ; F, G, H. L . hirticeps sp. nov. ; I, J, K. L . latifascia sp. nov. ; L. L . pinguifoliacea sp. nov. FIGURE 12. Abdominal tergite pattern in male: A. Leucophenga pinguifoliacea sp. nov. ; B. L . retihirta sp. nov. ; C. L . securis sp. nov. ; D, E, F. L . sinupenis sp. nov. ; G. L . villosa sp. nov. ; H. Color legend. FIGURE 13. Male terminalia: A, B. Leucophenga atrinervis ; C, D. L . digmasoma ; E, F. L . multipunctata ; G, H. L . nigrinervis ; I, J. L . pectinata . FIGURE 14. Male terminalia: A, B. Leucophenga pentapunctata ; C, D. L . quadripunctata ; E, F. L . regina ; G, H. L . trivittata ; I, J. L . acutifoliacea sp. nov. FIGURE 15. Male terminalia: A, B. Leucophenga albiterga sp. nov. ; C, D. L . angustifoliacea sp. nov. ; E, F. L . baculifoliacea sp. nov. ; G, H. L . cornuta sp. nov. ; I, J. L . hirticeps sp. nov. FIGURE 16. Male terminalia: A, B. Leucophenga latifascia sp. nov. ; C, D. L . pinguifoliacea sp. nov. ; E, F. L . retihirta sp. nov. ; G, H. L . securis sp. nov. ; I, J. L . sinupenis sp. nov. FIGURE 17. Male terminalia: A, B. Leucophenga villosa sp. nov. FIGURE 18. Leucophenga atrinervis , male terminalia in lateral view. A. epandrium (epand), surstylus (sur) and cercus (cerc); B. hypandrium (hypd), paramere (pm), gonopods (gon), aedeagus (aed) and aedeagal apodeme (aed a); C. paramere; D. aedeagus. Scale bars = 0.1 mm. Measurements. BL = 3.53–4.13 mm in ♂, 3.43–4.33 mm in ♀ (range in 5♂ and 5♀), THL = 1.60–2.13 mm in ♂, 1.63–2.13 mm in ♀, WL = 2.83–3.60 mm in ♂, 3.00– 3.93 mm in ♀, WW = 1.30–1.63 mm in ♂, 1.37–1.83 mm in ♀, arb = 6–8/3–4, avd = 0.76–0.93, adf = 1.41–1.76, flw = 1.45–1.78, FW/HW = 0.42–0.47, ch/o = 0.05–0.07, prorb = 0.64–0.90, rcorb = 0.62–0.94, vb = 0.31–0.42, dcl = 0.42–0.65, presctl = 0.44–0.73, sctl = 1.11–1.49, sterno = 0.64–0.89, orbito = 1.69–2.20, dcp = 0.24–0.36, sctlp = 0.92–1.35, C = 2.13–2.65, 4c = 1.23–1.53, 4v = 1.90–2.40, 5x = 0.96–1.14, ac = 2.41–2.88, M = 0.54–0.63, C3F = 0.81–0.86. Specimens examined. CHINA : 1♀ (SCAU, No. 122952 ), Guanmenshan, Benxi, Liaoning, 41˚13'N, 124°38'E , 400m , 27.vi.2009 , ex tree trunk, HW Chen; 22♂ , 23♀ (SCAU, Nos 122953 –97), Baiyunshan, Luoyang, Henan, 33°31'N , 111°17'E , 1200m , 29.vi.2005 , ex tree trunks, JJ Jiang, T Li; 1♂ , 1♀ (SCAU, Nos 123034 , 35), Maoniuhe, Danba, Sichuan, 30˚41'N, 101˚45'E, 2650m , 14.ix.2005 , ex tree trunks, HW Chen; 1♂ (SCAU, No. 123036 ), Wuliangshan, Jingdong, Yunnan, 18°41'N , 108°52'E , 1800m , 4.viii.2006 , ex tree trunk, T Li. JAPAN : 1♂ (SCAU, No. 122998 ), Izumimura, Kumamoto, 18.viii.2002 , tree trunk, HW Chen. Distribution. China (Liaoning, Henan, Sichuan, Yunnan), Japan (Honshu).