Morphological and molecular characterization of twenty-five new Diploneis species (Bacillariophyta) from Lake Tanganyika and its surrounding areas Author Jovanovska, Elena 0000-0002-3413-3683 Department of Paleoanthropology, Senckenberg Research Institute and Natural History Museum Frankfurt, Frankfurt, Germany & jovanovska. eci @ gmail. com; https: // orcid. org / 0000 - 0002 - 3413 - 3683 jovanovska.eci@gmail.com Author Wilson, Mallory C. 0000-0002-2852-125X Department of Paleoanthropology, Senckenberg Research Institute and Natural History Museum Frankfurt, Frankfurt, Germany & Indiana State University, Indiana State University, Terre Haute, IN, USA & mwilson 108 @ sycamores. indstate. edu; https: // orcid. org / 0000 - 0002 - 2852 - 125 X mwilson108@sycamores.indstate.edu Author Hamilton, Paul B. 0000-0001-6938-6341 Phycology Section, Research and Collections Division, Canadian Museum of Nature, Ottawa, Canada & phamilton @ nature. ca; https: // orcid. org / 0000 - 0001 - 6938 - 6341 phamilton@nature.ca Author Stone, Jeffery 0000-0002-1313-0643 Department of Paleoanthropology, Senckenberg Research Institute and Natural History Museum Frankfurt, Frankfurt, Germany & Indiana State University, Indiana State University, Terre Haute, IN, USA & Department of Paleoanthropology, Senckenberg Research Institute and Natural History Museum Frankfurt, Frankfurt, Germany & jeffery. stone @ indstate. edu; https: // orcid. org / 0000 - 0002 - 1313 - 0643 * Corresponding author & Department of Paleoanthropology, Senckenberg Research Institute and Natural History Museum Frankfurt, Frankfurt, Germany jeffery.stone@indstate.edu text Phytotaxa 2023 2023-04-21 593 1 1 102 http://dx.doi.org/10.11646/phytotaxa.593.1.1 journal article 10.11646/phytotaxa.593.1.1 ef558f00-24a4-4671-bf56-df3c1d61ecd1 1179-3163 7875089 Diploneis clara sp. nov . (LM Figs 384–408 , SEM Figs 409–418 ) Valves are weakly asymmetric, linear-elliptic with slightly convex margins and round ends ( Figs 384–409 ). Valve length is 17.5–43.5 μm and width is 9.5–16 μm. The axial area is linear to lanceolate, widening at the center to form a longitudinally elongate and weakly asymmetric central area ( Figs 386 , 409, 411 ), 2–3.5 μm wide. Externally, the canal is linear to lanceolate, slightly expanded in the middle of the valve with two rows of cribrate (ca. 15 poroids) areolae narrowing into one at the valve apices ( Figs 386 , 409–411, 413 ). Internally, a thick non-porous slightly raised silica plate encloses the longitudinal canal ( Figs 415, 418 ). Externally, the raphe is filiform, curved with simple and deflected proximal ends; a linear expanded teardrop depression contains the proximal ends ( Figs 409, 411, 412 ). The distal raphe ends are unilaterally bent to the same side as the proximal ends, terminating on the valve face at apex margin ( Figs 409, 410, 413, 414 ). Internally, the raphe is present in a depression formed by the longitudinal canal; the raphe is curved with simple proximal and distal ends that are slightly elevated in helictoglossae ( Figs 415, 418 ). The striae are parallel at mid-valve becoming radiate towards the apices, 10–12 in 10 μm. Striae are uniseriate becoming biseriate towards the valve margins (white arrow in Figs 410 , 417 ; Figs 409, 414 ). The alveolate striae are composed of round to rectangular areolae covered externally with a pored cribra (10–15 poroids), 15–25 in 10 μm. Each areola opens into shallow pits ( Fig. 411 ). The inter-areolar thickenings have serrated fin-like silica ridges (number of notched edges hardly visible on the illustrated images). The areolae increase in size towards the valve margins ( Figs 409, 413 ). Internally, the alveoli open via a single elongated opening covered with a thin silica layer ( Fig. 416 ). The valvocopula has serrated advalvar edges ( Figs 415, 416 ). Type:— REPUBLIC OF ZAMBIA , Lake Tanganyika , Chituta Bay , at 768 m elevation; mud and mussels, 33 m water depth, collected SCUBA diving, 8°42’56.0” S 31°09’15.0” E , W. Salzburger , 5 th September 2018 ( holotype designated here, circled specimen BM-108991! = Fig. 399 , isotypes ANSP-GC17220 !, CANA-129333!). Type material CANA-129321. Registration: http://phycobank.org/103699 Pictures of the isolated specimen:— LM micrograph on 1000× magnification ( Fig. S3z ). Sequence data:— Plastid gene rbc L sequence (GenBank accession: OQ 660281). Etymology:— The specific epithet ‘ clara ’ refers to bright, radiant appearance in the light microscope. Ecology and distribution:— Diploneis clara sp. nov. has only been observed in Lake Tanganyika in the three sub-basins on the Tanzanian and Zambian sides. The species is abundant in the alkaline, moderately mineral-rich and highly transparent lake. It is typically found on sand and mud (sometimes with shells) between 7.5 and 30 m water depth at Kalambo Falls Lodge, Isanga Bay, Chituta Bay, Mutondwe Island, Cape Nangu at Kasaba Bay, Ndole Bay, Mahale National Park, and Kiganza Bay (see Fig. 1c–f ). It has also been found on submerged rocks in the littoral areas at Jakobsen Beach near Kigoma , probably resulting from currents and water turbulence. This very common species cohabits with various Diploneis species at different sites, but mainly with D. cocquytiana sp. nov. , D. serrulata sp. nov. , D. kilhamiana sp. nov. , D. duplex sp. nov. , D. cristata sp. nov. , D. salzburgeri sp. nov. , and D. angusta sp. nov. Main differential characters:— Valve shape, striae pattern, external fin-like ornamentations across the valve, fins fine at mid-valve, and poroids 10–15 per areola. Similar species:— Diploneis angusta sp. nov. and Diploneis fontannella Lange-Bertalot in Werum & Lange-Bertalot (2004: 141) .