Polyphasic classification of Nonomuraea strains isolated from the Karakum Desert and description of Nonomuraea deserti sp. nov., Nonomuraea diastatica sp. nov., Nonomuraea longispora sp. nov. and Nonomuraea mesophila sp. nov. Author Saygin, Hayrettin Author Nouioui, Imen Author Ay, Hilal Author Guven, Kiymet Author Cetin, Demet Author Klenk, Hans-Peter Author Goodfellow, Michael Author Sahin, Nevzat text International Journal of Systematic and Evolutionary Microbiology 2020 2020-02-03 70 1 636 647 journal article 10.1099/ijsem.0.003808 e70e39cf-ffb6-4178-989c-6a42a3c0c3bf 1466-5034 3745026 DESCRIPTION OF NONOMURAEA MESOPHILA SP. NOV. Nonomuraea mesophila (me.so ′ phi.la Gr. n. mesos middle; Gr. fem. adj. philos loving; N.L. fem. adj. mesophila middle temperature loving). Aerobic, Gram-stain-positive, non-motile actinobacterium that forms extensively branched substrate and aerial mycelia. Aerial hyphae differentiate into spore chains with smooth surfaces. Good growth occurs on ISP 2, ISP 4, modified Bennett’s and tryptic soy agar and moderate growth on Czapek’s, nutrient and ISP 5–7 agar. A range of substrate mycelial pigments are formed including cream, light brown, dark brown, reddish brown, reddish black or claret red ones. Melanoid pigments are not produced on the ISP 6 agar. Red coloured diffusible pigments are observed on modified Bennett’s agar. Grows from 20–37 °C (optimum, 28 °C), from pH 6.0–8.0 (optimum, pH 7.0) and in the presence of up to 5 % (w/v) NaCl. Hydrolyses aesculin, reduces nitrate, but does not produce H 2 S, does not hydrolyse allantoin, arbutin or urea. Degrades hypoxanthine, starch and Tweens (20, 40 and 80), but not adenine, casein, chitin, gelatin, guanine, xanthine or xylan. Adonitol, D-arabinose, D-fructose, raffinose, cellobiose, D-galactose, melezitose, melibiose, dextran, D-glucose, inulin, lactose, D-maltose, D-mannitol, sucrose and D-xylose are used as sole carbon and energy sources but not D-sorbitol, D-mannose, dextrin, L-sorbose, L-arabinose, L-rhamnose, L-glutamine, myo-inositol, sodium succinate or xylitol. L-Alanine, L-arginine, L-asparagine, L-cysteine, glycine, L-hydroxyproline, α-isoleucine, L-methionine, L-phenylalanine, L-proline, L-serine, L-tyrosine and L-valine are utilized as sole nitrogen sources, but not L-histidine. The predominant menaquinones are MK-9(H 4 ) and MK-9(H 6 ). The polar lipid profile include diphosphatidylglycerol, phosphatidylmonomethylethanolamine, phosphatidylethanolamine, hydroxy-phosphatidylethanolamine, dihydroxy-phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol, two unidentified aminophospholipids, an unidentified glycophospholipid, four unidentified phospholipids and an unidentified lipid. Whole-cell hydrolysates include meso -A 2 pm, glucose, mannose, madurose and ribose. The major fatty acids are C 16:0 , iso-C 16:0 and C 17:0 10-methyl. The DNA G+C content of strain 6K102 T is 70.8 mol% and its genome size 10.14 Mbp. The type strain, 6K102 T (=CGMCC 4.7541 T =JCM 32916 T ), was isolated from desert soil sample collected from the Karakum Desert , Turkmenistan . The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain 6K102 T is MG770753 and draħ genome sequence accession number SMLD00000000 .