Systematic revision of the parasitoid wasp genus Glyptapanteles Ashmead (Hymenoptera: Braconidae: Microgastrinae) for Australia results in a ten-fold increase in species Author Fagan-Jeffries, Erinn P. C724E269-029E-49E8-8D95-6F5A5DA6BAAF Australian Centre for Evolutionary Biology & Biodiversity and School of Biological Sciences, The University of Adelaide, Australia. & South Australian Museum, Adelaide, Australia. erinn.fagan-jeffries@adelaide.edu.au Author McCLELLAND, Alana R. 3FDC78D1-CDF3-472F-B4EE-63A43C1730AF Australian Centre for Evolutionary Biology & Biodiversity and School of Biological Sciences, The University of Adelaide, Australia. alana.mcclelland@adelaide.edu.au Author Bird, Andrew J. DC97FEB2-1BB0-48CE-9178-0C5F98131CC0 Australian Centre for Evolutionary Biology & Biodiversity and School of Biological Sciences, The University of Adelaide, Australia. andrewbird@ozemail.com.au Author Giannotta, Madalene M. FF66BA72-4585-402F-AA42-61C9B7856048 Australian National Insect Collection, CSIRO, Black Mountain, ACT, Australia and Centre for Biodiversity Analysis, Research School of Biology, Australian National University, Acton, ACT, Australia. madalene.giannotta@gmail.com Author Bradford, Tessa M. D018F430-ED59-47BA-BF6A-EF8C6675AC20 Australian Centre for Evolutionary Biology & Biodiversity and School of Biological Sciences, The University of Adelaide, Australia. & South Australian Museum, Adelaide, Australia. tessa.bradford@samuseum.sa.gov.au Author Austin, Andrew D. DE71F924-750D-490D-84A7-F5960066F7CC Australian Centre for Evolutionary Biology & Biodiversity and School of Biological Sciences, The University of Adelaide, Australia. & South Australian Museum, Adelaide, Australia. andrew.austin@adelaide.edu.au text European Journal of Taxonomy 2022 2022-02-08 792 1 1 116 http://dx.doi.org/10.5852/ejt.2022.792.1647 journal article 20694 10.5852/ejt.2022.792.1647 0d881922-a259-4986-99d8-8fc3919204b0 2118-9773 6037052 18DB5F54-5CEB-498E-A6F1-E570E6A57833 Glyptapanteles arcanus Fagan-Jeffries, Bird & Austin sp. nov. urn:lsid:zoobank.org:act: FC2D5B3D-1AD2-478C-8920-8706F1BD594F Fig. 19 Diagnosis Glyptapanteles arcanus sp. nov. is in the G. arcanus species group and can be separated from the other members of the species group (other than from G. vergrandiacus sp. nov. ) by the propodeum with having coarser sculpturing than all but G. lambkinae sp. nov. and G. erucadesolator sp. nov. and with the T1 less strongly sculptured than in G. lambkinae sp. nov. and G. erucadesolator sp. nov. It is noted, however, that all these species (along with G. goodwinnoakes sp. nov. , which has T1 smooth) are morphologically very similar and identifications should be made by comparison of DNA barcodes. There was not a morphological character found to easily differentiate G. arcanus sp. nov. from G. vergrandiacus sp. nov. , which are sister lineages in the current phylogeny ( Fig. 2 ), but these two species differ in the wingless barcode by a single base pair and can also be separated by the COI barcodes, which have a>3% divergence. Etymology The species epithet ‘ arcanus ’ is a Latin adjective meaning ‘mysterious’ and refers to the cryptic nature of this species’ morphology, which has few distinctive features. Material examined Holotype AUSTRALIA • ♀ ; Queensland , Lamington National Park ; -28.207 , 153.137 ; 560 m a.s.l. ; 9–19 Mar. 2007 ; C. Lambkin and N. Starick leg.; IBISCA Plot # IQ-500-A rainforest Malaise trap , bulk vial 22028; Extraction1462, BOLD: AUGLY082-21; QM T250938 . Paratype AUSTRALIA • 1 ♀ ; Queensland , Lamington National Park ; -28.155 , 153.139 ; 282 m a.s.l. ; 13–23 Jan. 2007 ; C. Lambkin and N. Starick leg.; IBISCA Plot # IQ-300-B rainforest Malaise trap ; Extraction641, BOLD: AUMIC423-18; QM T208391 . Description Female COLOURATION. Gena without a pale spot; labrum reddish-brown; scape colour in ventral half uniformly paler than flagellomeres; flagellomeres all black/dark brown; tegula light brown; wing veins uniformly reddish-brown; anteromesoscutum dark; scutellar disk and metanotum dark with red tinge; propodeum dark; fore coxa dark; mid coxa dark; hind coxa dark; fore femur pale yellow; mid femur light brown; hind femur dark reddish-brown or orange to light brown; fore tibia pale yellow; mid tibia pale yellow; hind tibia darkening posteriorly; hind basitarsus light brown; T1 dark; T2 sclerotised area dark reddishbrown, dark area extending past indentation to non-sclerotised area, but T2 lateral area then pale; T3 mostly dark with paler lateral areas; T4+ reddish-brown. HOLOTYPE BODY MEASUREMENTS. Body length 2.3 mm ; fore wing length 2.3 mm ; antennal length similar to body length. HEAD. Antennal flagellomere 14 length/width 1.75–2.16; antennal flagellomere 2 length/width 3.57– 3.71; OOD/POD 1.86–2.17; IOD/POD 1.14–1.50. MESOSOMA. Anteromesoscutum sculpturing with shallow to deep punctures, space between punctures a mixture of smaller than diameter of punctures and of similar size, often smoother in posterior centre; scutellar disk sculpturing with shallow punctures scattered over most of area; 8–9 pits in scutellar sulcus. Propodeum with median carina absent, propodeum coarsely rugose. WINGS. Pterostigma length 0.59 mm ; pterostigma width 0.12 mm ; r 0.17 mm ; 2RS 0.12 mm ; 2m 0.1 mm ; (RS+M)b 0.08 mm . Fig. 19. Glyptapanteles arcanus Fagan-Jeffries, Bird & Austin sp. nov. , paratype, ♀ (QM T208391). A . Lateral habitus. B . Lateral head. C . Dorsal head. D . Anterior head. E . Dorsal habitus. F . Fore wing. METASOMA. T1 lateral edges parallel for anterior ½ to ⅔ of length, then narrowing posteriorly or broadest at centre of length, narrowing both posteriorly and anteriorly from mid-point; T1 smooth and shiny, some shallow scattered punctures on lateral edges and posterior half or indistinct sculpturing for most of length; T1 length 0.44 mm ; T1 width at posterior edge 0.1 mm ; T2 an isosceles trapezoid, lateral edges straight; T2 smooth and shiny; T2 length 0.12 mm ; T2 width at posterior edge 0.28 mm ; ovipositor slightly protruding from end of metasoma. Male Unknown. Remarks The wingless barcode for this species is shared with G. doreyi sp. nov. (one haplotype, as there are two wingless haplotypes present within G. doreyi sp. nov. ) and G. goodwinnoakes sp. nov. and differs by 1 bp from the barcodes of G. vergrandiacus sp. nov. , G. doreyi sp. nov. (second haplotype), and G. lessardi sp. nov. This group of species are very closely related and would benefit from future, more detailed taxonomic work to ensure that they are not simply divergent populations of the same species. For this species hypothesis, we have made the decision to split it into different species based on the COI barcode sequences being>3% divergent; however, we note that this is a hypothesis and is open to change with future work. Glyptapanteles arcanus sp. nov. constitutes BIN BOLD:ADL5262 and is 3.67% (p-dist.) divergent from the closet BIN in the database (BOLD:ADL3012; Glyptapanteles vergrandiacus sp. nov. ). Using the BOLD Batch ID engine, the COI sequence of the holotype is 3.5% different from the most similar COI sequence from an Australian specimen (AUGLY102-21; Glyptapanteles vergrandiacus sp. nov. ). Distribution This species is known only from Lamington National Park in southern QLD.