Arachidicoccus ginsenosidivorans sp. nov., with ginsenosideconverting activity isolated from ginseng cultivating soil Author Siddiqi, Muhammad Zubair Author Aslam, Zubair Author Im, Wan-Taek text International Journal of Systematic and Evolutionary Microbiology 2017 2017-04-01 67 4 1005 1010 http://dx.doi.org/10.1099/ijsem.0.001720 journal article 20554 10.1099/ijsem.0.001720 b06ff60a-17b8-4ac9-84b8-bc63f2963e86 1466-5034 6223652 DESCRIPTION OF ARACHIDICOCCUS GINSENOSIDIVORANS SP. NOV. Arachidicoccus ginsenosidivorans (gin.se.no.si.di.vo′ rans. N. L. n. ginsenosidum ginsenoside; L. part. adj. vorans eating, devouring; N.L. part. adj. ginsenosidivorans ginsenosidedevouring). Cells are Gram-reaction-negative, aerobic, non-motile and rod shaped (width, 0.4–1 µm; length, 1.5–3 µm). Colonies grown at 30 Ǫ C on nutrient agar for 2 days are light yellow, smooth, convex, opaque, circular with regular margins, and 2–3.5 mm in diameter. Growth occurs at 10–37 Ǫ C and pH 5–7 with 0–4 % NaCl (w/v). Optimum growth occurs at 30 Ǫ C and pH 7.0 with 0–1 % NaCl (w/v). Indole production is negative. In the API kits (API 20 NE, 32 GN and API ZYM), positive for the reduction of nitrate, and alkaline phosphatase, esterase, leucine arylamidase, valine arylamidase, cystine arylamidase, acid phosphatase, napthol-AS-BI-phosphohydrolase, a- galactosidase, b- galactosidase, a- glucosidase, N -acetyl-b- glucosaminidase and a - fucosidase activties. L- Rhamnose, sucrose, L- arabinose and melibiose are utilized. Negative for lipase, trypsin, b- glucuronidase, a- mannosidase, L- tryptophan, urease and gelatin hydrolysis. Does not utilize maltose, D- ribose, inositol, itaconic acid, suberic acid, sodium malonate, sodium acetate, lactic acid, L- alanine, potassium 5-ketogluconate, glycogen, 3-hydroxybenzoic acid, L- serine, D- mannitol, capric acid, valeric acid, trisodium citrate, potassium 2-ketogluconate, 3-hydroxybutyric acid, 4- hydroxybenzoic acid, potassium gluconate, adipic acid, malic acid and phenylacetic acid. MK-7 is the predominant respiratory quinone and iso-C 15: 0 , iso-C 15: 1 G, iso-C 17: 0 3- OH and summed feature 3 (comprising iso-C 16: 1 Ɯ 7 c and/ or C 16: 1 Ɯ 6 c ) are the major cellular fatty acids. The polar lipid profile is composed of PE, six unknown polar lipids (L1–L6) and an unknown aminolipid. TLC analysis shows that the type strain Gsoil 809 T converts major ginsenoside Rg1 (PPT-type) to minor ginsenoside Rh1, while the ginsenoside Re is not changed (Fig. S5). Table 2. Cellular fatty acid contents (%) of strain Gsoil 809T and the type strain of the phylogenetically related species of the genus Arachidicoccus Strain: 1, Gsoil 809T; 2, A. rhizosphaerae KCTC 22378T. Both strains were cultured on nutrient agar for 48 h at 30 Ǫ C. Results are presented as percentages of the total fatty acids. – , Not detected. The type strain, isolated from ginseng cultivating soil, Republic of Korea , is Gsoil 809 T (= KCTC 22820 T = JCM 30984 T ). The G +C content of the genomic DNA of the type strain is 39.4 mol%. The polyamines are homospermidine and methylbenzoate.