A reassessment of the Neotropical genus Pseudonannolene Silvestri, 1895: cladistic analysis, biogeography, and taxonomic review (Spirostreptida: Pseudonannolenidae) Author Iniesta, Luiz Felipe Moretti DEEF048E-97FB-4CCD-875F-5FA6184CA8AB&14A15A7F-730F-4D41-BDAC-D53514FAB85D Pós-graduação em Zoologia, Instituto de Biociências, Universidade de São Paulo, São Paulo, Brazil. & Laboratório de Coleções Zoológicas, Instituto Butantan, Avenida Vital Brasil, 1500, 05503 - 090, São Paulo, Brazil. rodrigobouzan@outlook.com Author Bouzan, Rodrigo Salvador DEEF048E-97FB-4CCD-875F-5FA6184CA8AB&14A15A7F-730F-4D41-BDAC-D53514FAB85D Pós-graduação em Zoologia, Instituto de Biociências, Universidade de São Paulo, São Paulo, Brazil. & Laboratório de Coleções Zoológicas, Instituto Butantan, Avenida Vital Brasil, 1500, 05503 - 090, São Paulo, Brazil. rodrigobouzan@outlook.com Author Brescovit, Antonio Domingos 14A15A7F-730F-4D41-BDAC-D53514FAB85D&D5B81D79-AFAE-47B1-8A6E-DAB448A24BCC Laboratório de Coleções Zoológicas, Instituto Butantan, Avenida Vital Brasil, 1500, 05503 - 090, São Paulo, Brazil. rodrigobouzan@outlook.com&antonio.brescovit@butantan.gov.br text European Journal of Taxonomy 2023 2023-04-27 867 1 1 312 http://dx.doi.org/10.5852/ejt.2023.867.2109 journal article 57602 10.5852/ejt.2023.867.2109 1d2570da-e150-4d75-94d8-bfc5813062bc 2118-9773 7891021 8DEF295C-A8B1-4A6B-B873-B30949F64E07 Pseudonannolene segmentata Silvestri, 1895 Figs 123–124 , 172C , 178M , 188 Pseudonannolene segmentata Silvestri, 1895b: 7 . Pseudonannolene segmentata – Silvestri 1902: 19 (description of female topotype). — Viggiani 1973: 367 . — Jeekel 2004: 91 . — Iniesta & Ferreira 2013a: 92 ; 2013b: 366 . Diagnosis Males of P. segmentata slightly resemble those of P. bucculenta sp. nov. and P. morettii sp. nov. by having the internal branch narrow, foliaceus ( Fig. 124D–F ), but differing by the short prefemoral process on the first leg-pair ( Fig. 124A ); solenomere with ectal process subtriangular, separated from the apicomesal process by a shallow notch ( Fig. 124D ). Etymology Named after the Latin adjective ‘ segmentatus ’ = ‘adorned with borders or patches’. Unspecified in the original description. Material examined (total: 9 ♂♂ , 6 ♀♀ , 9 immatures ) BRAZIL – Mato Grosso do Sul • 1 ♂ , 1 ♀ immature; Bonito , Pitangueira ; [ -21.136212 , -56.485720 ]; 297 m a.s.l. ; Oct. 2002 ; C.A. Rheims leg.; IBSP 1929 • 1 ♂ , 1 immature ; same locality data as for preceding; V.C. Onofre leg.; IBSP 1928 • 1 ♂ , 1 ♀ , 2 ♀♀ immatures; same collection data as for preceding; IBSP 1931 • 1 ♂ , 1 ♀ ; same collection data as for preceding; I. Cizauskas leg.; IBSP 1930 • 1 ♀ ; Bonito ; [ -21.128974 , -56.481720 ]; 294 m a.s.l. ; 14–23 Oct. 2002 ; Equipe Biota leg.; IBSP 2592 • 1 ♀ ; same collection data as for preceding; IBSP 2599 • 1 ♀ immature; same collection data as for preceding; IBSP 2601 • 1 ♂ ; same collection data as for preceding; IBSP 2605 • 1 ♀ ; same collection data as for preceding; IBSP 2583 • 1 ♀ ; same collection data as for preceding; IBSP 2603 • 1 ♂ ; same collection data as for preceding; IBSP 2602 • 2 ♂♂ , 2 ♀♀ immatures; same collection data as for preceding; IBSP 2609 • 1 ♂ , 2 ♀♀ immatures; same collection data as for preceding; 16 Jul. 1992 ; E. Trajano and P. Gnaspini leg; MZSP . Descriptive notes MEASUREMENTS . 55–62 body rings (1–2 apodous + telson). Males: body length 45.6–52.5 mm ; maximum midbody diameter 2.8–4.1 mm . Females: body length 37.4–42.5 mm ; maximum midbody diameter 2.7–3.1 mm . COLOR . Body color reddish brown; head, antennae, and collum little darker; prozonites anteriorly greyish; metazonites with a medial brown band and a posterior lighter; legs yellowish brown. HEAD . Antennae short ( Fig. 123A ), just reaching back to end of ring 5 when extended dorsally; relative antennomere lengths 1<2≈3>4>5≈6>7. Mandibular cardo with ventral margin narrow. Ommatidial cluster well-developed, elliptical; ca 25 ommatidia in 5 rows. BODY RINGS . Collum with lateral lobes broadly rounded, with ca 8 striae, curved ectad ( Fig. 123A ). Very faintly constricted between prozonite and metazonite; prozonites smooth; metazonites laterally with transverse striae below ozopore. Anterior sterna in midbody rings subrectangular, without transverse striae ( Fig. 172C ). FIRST LEG-PAIR OF MALES . Coxae ( cx ) short (less than half of remaining podomere lengths), subtriangular, densely setose ( Fig. 124A ); prefemoral process ( prf ) about as wide as half of prefemur, subcylindrical, densely setose up to its median region ( Fig. 124B ); remaining podomeres with setae along the mesal region. SECOND LEG-PAIR OF MALES . Coxa ( cx ) large and rounded; penis ( pn ) located at proximal region, rounded, not extended basally ( Fig. 124C ); prefemur compressed dorsoventrally; remaining podomeres setose. GONOPODS . Gonocoxa ( gcx ) elongated, almost twice as long as telopodite, with the base slightly arched; antero-posteriorly flattened ( Fig. 124D–F ); with rows of papillae mesally. Seminal groove ( sg ) curved; arising medially on mesal cavity and terminating apically on the seminal apophysis ( sa ). Shoulder ( sh ) subtriangular. Telopodite ( tp ) as wide as half of gcx ( Fig. 124D ); solenomere ( sl ) with apicomesal process ( amp ) short, rounded; ectal process ( ep ) subtriangular, perpendicular to amp ; sa located at mesal portion, visible apically. Internal branch ( ib ) narrow, foliaceus; almost not surrounding basally tp ; ib with setae along its entire margin exceeding apically seminal region of sl ( Fig. 124D–F ). VULVAE . As typical for the genus. Bursa subtriangular, glabrous ( Fig. 178M ); internal valve subtriangular, with mesal region rounded; operculum slightly curved ectad, expanded apically; external valve wide, subtriangular. Distribution Known from the Cerrado biome (tropical savanna ecoregion) on the border of the Brazilian state of Mato Grosso do Sul and the Paraguayan department of Concepción ( Fig. 188 ). Comments The type material described by Silvestri (1895b) was not found after consulting the Museo Regionale Scienze Naturali, Torino, Italy (MRSN). Nevertheless, topotypes from surrounding areas in the Apa River were examined ( Fig. 188 ). Other specimens from Apa River in Paraguay were also recorded by Silvestri (1902) .