Sulcisporasupratumida sp. nov. (Phaeosphaeriaceae, Pleosporales) on Anthoxanthumodoratum from Italy Author Senanayake, Indunil C. Author Jeewon, Rajesh Author Camporesi, Erio Author Hyde, Kevin D. Author Zeng, Yu-Jia Author Tian, Sheng-Li Author Xie, Ning text MycoKeys 2018 38 35 46 http://dx.doi.org/10.3897/mycokeys.38.27729 journal article http://dx.doi.org/10.3897/mycokeys.38.27729 1314-4049-38-35 Sulcispora supratumida Senan., Camporesi & K.D. Hyde sp. nov. Figure 2 Etymology. The species epithet is based on the two Latin words "supra" meaning upper and "tumidus" meaning swollen, referring to the position of swollen cells of ascospores. Type. ITALY. Province of Forli-Cesena, Premilcuore, Passodella Valbura, on dead leaves of Anthoxanthum odoratum L. ( Poaceae ), 25 May 2013, Erio Camporesi, IT 1306 (MFLU 15-0038, holotype; HKAS 83865, paratype): living cultures, MFLUCC 14-0995. Description. Saprobic on leaves of Anthoxanthum odoratum L., visible as black spots, occurring on the upper surface of entire leaf. Sexual morph. Ascomata 110-150 x 90-140 µm ( x- = 140-125 µm , n = 10), scattered, solitary, immersed, uniloculate, globose, black. Ostiole 35-40 µm ( x- = 39 µm , n = 10) wide, papillate, central, periphysate. Periphyses 15-20 µm long, hyaline. Peridium comprising 2-4 layers of brown to dark brown, thick-walled, cells of textura angularis to textura globularis. Hamathecium comprising 2-4 µm wide, cellular, hyaline, branched, septate, pseudoparaphyses, constricted at the septa, anastomosing mostly above the asci and embedded in a mucilaginous matrix. Asci 85-125 x 20-35 µm ( x- = 100 x 30 µm , n = 20), 8-spored, few, bitunicate, fissitunicate, subglobose to clavate, short pedicellate, apically rounded, with an ocular chamber, arising from the base of the ascoma and attached to parenchymatous cell matrix at base. Ascospores 30-35 x 6-9 µm ( x- = 35 x 7 µm , n = 25), bi-seriate to tri-seriate, narrowly fusiform, narrowing towards the end cells, reddish to dark brown, 6-septate, second septum supra-median, slightly constricted, not constricted at other septa, second segment swollen, straight, with 12-16 longitudinal furrows on surface, lacking a mucilaginous sheath. Asexual morph . Undetermined. Figure 2. Sulcispora supratumida (MFLU 15-0038). a Leaves of Anthoxanthum odoratum b Appearance of ascomata on host surface c Cross section of ascoma d Peridium e Pseudoparaphyses f-i Asci j-n Ascospores o Upper surface of the culture p Lower surface of the culture. Scale bars: 200 µm (b), 50 µm (c), 20 µm ( d-i ), 10 µm ( j-n ). Culture characteristics. 2 cm diameter after 4 weeks incubated in dark at 25 °C on MEA, pinkish-white, circular, slightly woolly, margin lobate, effuse, lacking aerial mycelium, tightly attached to the media.