Anatolian endemic genus Bolua (Orthoptera: Tettigoniidae: Tettigoniinae): genetic and phenotypic data indicate inconsistent diversity and evolutionary patterns Author Uluar, Onur 0000-0002-5039-8024 Department of Biology, Faculty of Science, Akdeniz University, 07058 Antalya, Turkey. & onuruluar @ gmail. com; https: // orcid. org / 0000 - 0002 - 5039 - 8024 onuruluar@gmail.com Author Yahyaoğlu, Özgül 0000-0001-7965-7117 Department of Biology, Faculty of Science, Akdeniz University, 07058 Antalya, Turkey. & ozgulyahyaoglu @ gmail. com; https: // orcid. org / 0000 - 0001 - 7965 - 7117 ozgulyahyaoglu@gmail.com Author Çiplak, Battal Department of Biology, Faculty of Science, Akdeniz University, 07058 Antalya, Turkey. text Zootaxa 2022 2022-10-12 5195 3 224 240 journal article 163378 10.11646/zootaxa.5195.3.2 fe55986d-056b-4413-b38d-b0bfa32549db 1175-5326 7187756 78D4FF8E-52AF-4941-A9D1-4A4F1D92A0D8 Bolua turkiyae Ünal, 1999 ( Figures 1–3, 6, 9, 12, 15 , 18, 21, 24 , 27 , 31 ; Tables 1–5 ) Bolua turkiyae Ünal ; 1999: 246 Type information: Holotype male, TURKEY : Bolu , Gerede , Aktas II , 1200 m , 21.08.1996 , leg. M. Ünal (coll. M. Ünal in Abant Izzet Baysal University, Bolu , Turkey ) . Paratypes 7 males , 11 females , same data as holotype ; 8 males , 5 females , same locality as holotype, 4.08.1995 , leg M. Ünal (coll. M. Ünal in Abant Izzet Baysal University, Bolu , Turkey ) . Material examined: 10 males , 8 females TURKEY : Çankırı , Çerkes , Isık Dağı , 1050–1250 m , 12.08.1996 (leg. B. Çıplak ) ( 1 male , 1 female in The Natural History Museum , London (E-1997/322), others in AUZM ) ; 2 males , 5 females , TURKEY : Kastamonu , Araç , Sıragömü yaylası, 900 m , 11.09.1998 (leg. B. Çıplak ) ( AUZM ) ; 4 males , 8 females , TURKEY , Bolu , Yedigöller National Park , 40.8804 °N , 31.68140°E , 1725m , 15.08.2010 (leg. B.Çıplak , S.Kaya & Z.Boztepe ) ( MEVBIL ) ; 16 males , 11 females , TURKEY , Kastamonu , Safranbolu , Karaevler , 41.34805°N , 32.69805°E , 996 m , 17.07.2017 (leg. B. Çıplak & O. Uluar ) ( MEVBIL ) ; 2 males , 2 females , TURKEY , Kastamonu , Şenpazar-Ağlı road, 41.724°N , 33.48833°E , 997m , 18.07.2017 ((leg. B. Çıplak & O. Uluar ) ( AUZM ) ; 2 males , 3 females , TURKEY , Kastamonu , Yenice , 41.23168°N , 33.79239°E , 1025m , 13.08.2004 (leg. D. Berger , D. Şirin , M.S. Taylan ) ( MEVBIL ) ; 1 male , 4 females ; TURKEY , Karabük , Keltepe , 41.0555°N , 32.468°E , 1453m , 16.07.2017 (leg. B. Çıplak & O. Uluar )( MEVBIL ) . FIGURES 3–17. 3–5. Male head+pronotum+tegmina in Bolua (3— B. turkiyae , 4— B. balıkesirensis , 5— B. bursaensis ; D— dorsal view, L—lateral view). 6–8 . Male anal tergite in Bolua (6— B. turkiyae , 7— B. balıkesirensis , 8— B. bursaensis ). 9–11 . Male cercus in Bolua (9— B. turkiyae , 10— B. balıkesirensis , 11— B. bursaensis ) . 12–14 . Male subgenital plate in Bolua (12— B. turkiyae , 13— B. balıkesirensis , 14— B. bursaensis ). 15–17 . Titillators in Bolua (15— B. turkiyae , 16— B. balıkesirensis , 17— B. bursaensis ) Description, diagnosis and remarks: Description of the type species Bolua turkiyae is given in Ünal (1999) and supplemented by Çıplak & Heller (2001) . Data presented here (see Figures 3, 6, 9, 12, 15 , 18, 21, 24 , 27 ) are consistent with these descriptions. This species can be distinguished from B. balikesirensis sp. n. by male cercus (compare Figures 9 and 10 ). However, differences between B. turkiyae with B. bursaensis sp. n. are not prominent, but they can be identified by male cercus again (compare Figures 9 and 11 ). Apart from the qualitative morphology, the PCA analyses applied to female measurements distinguished B. turkiyae and B. bursaensis sp. n. from each other ( Figure 27 ). Along with morphological differences, B. turkiyae corresponding PG 1 in phylogenetic tree ( Figure 2 ) is definable by 53 unique mutations ( Figure 31 ). The high net pairwise distance and F ST values with the other two phylogroups also support its distinctness as a species ( Table 3 ). Although Çıplak & Heller (2001) presented and described a male calling song for this species, that song record is from Uludağ— Bursa Province , the type locality of the new species described here, P. bursaensis sp. n. Presently song of this species is not available to study and to compare with the other two. This species occupies the eastern part of the generic range in the western part of the Anatolian Black Sea Basin ( Figure 1 ).