A checklist of the bats of Peninsular Malaysia and progress towards a DNA barcode reference library Author Voon-Ching Lim Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur, Malaysia voonchinglim@gmail.com Author Rosli Ramli Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur, Malaysia Author Subha Bhassu Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur, Malaysia, Author John-James Wilson International College Beijing, China Agricultural University, Beijing, P. R. China wilso04@gmail.com text PLoS ONE 2017 2017-07-25 12 7 1 65 journal article 10.1371/journal.pone.0179555 6ea2cc5c-857b-4b47-8135-8bff7efbd1fc PMC5526618 28742835 4251352 Rhinolophus stheno Andersen, 1905 Rhinolophus stheno Andersen, 1905: 91 , pl. 3; Selangor , MALAYSIA ( H. N. Ridley , Esq. , presenter; BM(NH) 98.3.13.1) [ 147 ]. Common English name: Lesser Brown Horseshoe Bat Barcode Index Number: DNA barcodes recorded as R . stheno are associated with two BINs, BOLD :AAO1567 and BOLD :ABY7284, but there are no DNA barcodes from Peninsular Malaysia in these BINs. Two DNA barcodes recorded as R . stheno ( BM 504-04 and BM 505-04) are from Peninsular Malaysia but are not placed in any BIN due to short sequence length ( < 500bp) Based on our NJ analysis, neither of the Peninsular Malaysia barcodes are associated with BOLD :AAO1567 or BOLD :ABY7284 ( Fig 5 ). Remarks: R . microglobosus was described as a subspecies of R . stheno based on its smaller skull and globular anterior median rostral swellings [ 167 ]. The taxa were later found to be morphometrically and acoustically distinct, and R . microglobosus was consequently raised as a distinct species with a distribution covering Thailand , Myanmar , Cambodia , Vietnam and Laos whereas R . stheno is restricted to southern Thailand , Peninsular Malaysia and central Vietnam [ 168 ]. Therefore, DNA barcodes recorded as R . stheno associated with the BIN, BOLD:AAO 1567 may represent R . microglobosus , and DNA barcodes, BM504-04 and BM505- 04 [ 4 ] may represent the R . stheno sensu stricto as they were collected at Kuala Lompat, Pahang, close to the type locality. The BIN, BOLD:ABY7284 which contains DNA barcodes recorded as R . stheno and R . shameli may be erroneous ( Fig 5 ). IUCN status: Least Concern Recorded at: Pahang : Krau Wildlife Reserve [ 11 , 42 ], Tasik Chini [ 43 ], Lata Bujang Forest Reserve [ 56 ], Cameron Highland [ 60 ], Bukit Ibam, Kemasul, Jengka, Pulau Tioman [ 79 ], Klau Besar, Kenong and Gunung Aais [ 100 ]; Pulau Pinang : Bukit Panchor [ 23 , 100 ]; Perak : Temengor Forest Reserve [ 46 , 47 , 111 ], Royal Belum State Park [ 66 ], Kledang Saiong Forest Reserve [ 101 ]; Perlis : Wang Kelian State Park [ 50 ]; Selangor : Bukit Kutu Wildlife Reserve [ 51 ], Ulu Gombak [ 54 , 101 ], Semangkok Forest Reserve [ 101 ]; Kedah : Ulu Muda Forest Reserve [ 57 , 100 ], Bukit Hijau [ 100 ]; Kelantan : Air Panas-Gua Musang [ 61 ], Gua Musang [ 62 ], Gunung Stong State Park [ 100 ]; Johor : Gunung Panti and Labis Forest Reserve [ 100 ]; Negeri Sembilan : Gunung Angsi Forest Reserve [ 100 , 101 ], Berembun Forest Reserve [ 101 ]; Terengganu : Gunung Tebu Forest Reserve [ 101 ] . R . stheno roosts in limestone caves and sometimes in smaller colonies in rock crevices and tree hollows [ 11 , 14 ]. Individuals have been reported roosting with R . lepidus [ 11 ].