A checklist of the bats of Peninsular Malaysia and progress towards a DNA barcode reference library Author Voon-Ching Lim Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur, Malaysia voonchinglim@gmail.com Author Rosli Ramli Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur, Malaysia Author Subha Bhassu Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur, Malaysia, Author John-James Wilson International College Beijing, China Agricultural University, Beijing, P. R. China wilso04@gmail.com text PLoS ONE 2017 2017-07-25 12 7 1 65 journal article 10.1371/journal.pone.0179555 6ea2cc5c-857b-4b47-8135-8bff7efbd1fc PMC5526618 28742835 4251352 Kerivoula minuta Miller, 1898 Kerivoula minuta Miller, 1898: 321 ; Lay Song Hong , Trang , south THAILAND ( Dr. W. L. Abbott , collector; USNM 83547 ) [ 174 ]. Common English name: Least Woolly Bat Barcode Index Number: BOLD : AAC 1298 (9 DNA barcodes from Peninsular Malaysia ; Fig 6 ) Remarks: DNA barcodes recorded as K . minuta are associated with six BINs, BOLD: AAC1296, BOLD:AAC1297, BOLD:AAC1298, BOLD:AAC1299, BOLD:ACF4510, and BOLD:ACF451 ( Fig 6 ). Khan [ 175 ] reported 4.44% of divergence in cytochrome b mtDNA between K . minuta from Peninsular Malaysia and Malaysian Borneo ( Sabah and Sarawak ) with no shared haplotypes. Our NJ analysis also showed a divergence between K . minuta from Peninsular Malaysia and Borneo ( Fig 6 ). The taxon occurring in Peninsular Malaysia represents K . minuta based on the type locality. IUCN status: Near Threatened Recorded at: Johor : Endau Rompin National Park (BM422-04 and ABRSS347-06 [ 4 ]), Gunung Panti and Labis Forest Reserve [ 100 ]; Selangor : Ulu Gombak and Hulu Langat [ 32 ]; Pahang : Krau Wildlife Reserve and Lakum [ 32 ], Kuala Atok, National Park [ 32 , 44 ], Bukit Ibam, Kenong and Gunung Aais [ 100 ]; Perak : Maxwell Hill [ 32 ], Temengor Forest Reserve [ 46 , 47 ], Royal Belum State Park [ 66 ]; Kedah : Bukit Hijau and Ulu Muda Forest Reserve [ 32 , 57 , 100 ]; Kelantan : Gua Musang [ 61 , 62 ]; Negeri Sembilan : Gunung Angsi Forest Reserve [ 100 ]. K . minuta has been recorded in the understory of lowland forests and disturbed areas [ 14 ]. Kerivoulapapillosa and K . lenis . K . lenis iscloselyassociatedwith K . papillosa [ 9 ] butthe former has a smaller skull and smaller teeth, shorter muzzle and narrower palate [ 169 , 176 ]. The two species are 10.85% divergent in cytochrome b mtDNA and possess unique karyotypic characters: K . papillosa has a diploid number of chromosomes = 38 and fundamental number = 54 whereas K . lenis has a diploid number of chromosomes = 38 and fundamental number = 52 [ 32 , 175 ]. Analyses of COI mtDNA by Francis et al. [ 4 ] suggested that there are at least four distinct clusters among specimens recorded as K . papillosa and K . lenis . Douangboubpha et al. [ 170 ] reported that specimens from Thailand referred as K . papillosa represent five morphological forms but only three distinct clusters based on COI mtDNA analyses.