A new species of Neostenoptera (Diptera: Cecidomyiidae: Winnertziinae) from eastern North America Author Plakidas, John D. Author Ferro, Michael L. text Insecta Mundi 2016 2016-10-21 2016 510 1 9 journal article 10.5281/zenodo.5170875 1942-1354 5170875 2A31F4CB-356E-408E-93EB-7CC1FE91E3E7 Neostenoptera appalachiensis Plakidas and Ferro , new species ( Fig. 1–13 ) Diagnosis. Adult ( Fig. 1 ), body length (measured from head to end of genitalia) male 1.3 mm (n=4); female 1.8–2.0 (n=3) mm. Color: antennae light brown; head, thorax, and legs brown; eyes reddish brown, with a dark brown spot below each eye; abdomen light brown; wings teneral, with only veins R 1 and CuA 1 present, and a fringe of long, brown setae along wing margin except on proximal 1/4. Males of N . kiefferi differ from males of this new species by their flagellomere 11 which is more broadly rounded on its base, and the tarsomere ratio is 1: 0.5: 0.4: 0.7. In addition, N. congoensis differs from this new species in that vein R 5 is present at midlength, the scutellum possesses two setae, and females have digitate sensoria on flagellomeres 1–8 and foreleg tarsomere ratio of: 1: 0.6: 0.5: 0.7 (female foreleg tarsomere ratio in N. appalachiensis is 1: 0.4: 0.3: 0.5). Head. Eyes situated laterally, separate, without an eye bridge. Occiput with two stout setae. Palpus and labellum absent; oral opening small ( Fig. 2 ). Frons with 2–3 setae, no scales. Antennal scape with one seta; pedicel with horizontal rows of microtrichia, no setae ( Fig. 3, 4 ); flagellum of female with10 flagellomeres with 2 digitate sensoria on flagellomeres 1–9 ( Fig. 4, 5, 6 , only ventral sensoria shown), each with a single lateral seta and short necks, flagellomere10 shorter, narrower than preceding flagellomeres ( Fig. 6 ); flagellum of male with 11 flagellomeres with either digitate sensoria or simple sensoria on flagellomeres 1–2 or 1–3, flagellomeres 4–11 lack sensoria ( Fig. 3, 7 ). Thorax and chaetotaxy. Two dorsolateral, 1–2 dorsocentral setae; 4 shorter setae on pronotum; lateral sclerites bare except for a fine covering of microtrichia. Wing ( Fig. 1 ) elongate, narrow, with a row of long brown setae along the entire wing margin except on proximal ¼; vein R 1 closely joined to C, reaching the wing margin before midlength, vein R 5 absent; vein CuA 1 visible near wing base. Halter with a single long seta near its base. Legs with fore femur inflated distally, mid, hind femora slender, or only slightly inflated distally; tarsi 4-segmented, tarsomere 1 about twice as long as 2, T1>T2>T3<T4, tarsal claws falcate, empodia shorter than claws ( Fig. 8 ); male foreleg tarsal ratio (excluding tarsal claw): 1: 0.4: 0.3: 0.5; female foreleg tarsal ratio (excluding tarsal claw): 1: 0.4: 0.3: 0.5. Male abdomen and chaetotaxy. Tergites, and sternites membranous. Tergite 1 with 2 lateral setae; tergites 2–7 with 2 setae situated anteriorly and posteriorly, and 2 closely approximated trichoid sensillae; tergite 8 ( Fig. 9 ) with 2 setae and 2 trichoid sensillae. Sternite 1 membranous, without setae; sternites 2–7 with 4 setae and 2 closely approximated trichoid sensillae; sternite 8 with 2 setae and 2 trichoid sensillae. Tergite 9 (epandrium) with 5–6 setae along posterior margin, no lateral setae or trichoid sensillum ( Fig. 9 ). Cerci elliptical ( Fig. 9 ), hypoproct (sternite 10) not discernible. Gonocoxites fused along anterior margin ( Fig. 10 ), covered with microtrichia and lateral, ventral setae. Gonostyli about 3 x longer than wide ( Fig. 9, 10 ), covered with fine setulae and a few setae, inner margin with longer, stouter hair-like setae forming a dense comb. Ejaculatory apodeme brown, heavily sclerotized, extending above the tegmen and longer than gonocoxal apodemes ( Fig. 10 ). Tegmen positioned dorsal to ejaculatory apodeme ( Fig. 10 ), heavily sclerotized, interrupted basally by a series of horizontal ridges ( Fig. 10, 11 ), and fused basally with the gonocoxal apodemes. Aedeagus membranous the apex extends beyond the tip of ejaculatory apodeme ( Fig. 10, 11 ). Female abdomen and chaetotaxy. Tergites, sternites 1–7 similar to male, except for distortion due to slide mounting, and therefore the trichoid sensillae are not visible. Tergite 8 with 2 anterior, 2 posterior setae; sternite 8 with 4 setae and one seta positioned laterally ( Fig. 12 ); other tergites, sternites with more numerous setae and a group of lateral setae ( Fig. 12 ). Hypoproct bilobed; cerci two segmented ( Fig. 12 ), disticercus slightly longer than basicercus, with horizontal rows of microtrichia and 2 short dorsal setae located at the distal corners ( Fig. 12 ). Two ovoid, lightly sclerotized spermathecae at level of tergite 8. Type material. Holotype male, labeled: USA : TN: Sevier Co. GSMNP Sugarlands QW , 24 VI-15 VII 2006 , N35˚39.826’, W83˚31.509’, reared 1- M. Ferro. Deposited in USNM . Six paratypes : 3 males , 3 females , with same pertinent data as the holotype. One female deposited in USNM ; 1 female deposited in LSAM ; 1 male , 1 female deposited in CUAC ; and 1 male , 1 female deposited in JPPC . Additional non-paratype material from USNM : 3 females , 1 male , Alabama , Baldwin Co. Shell mounds, Malaise trap , 5/19/2006 , John McCreadle , mounted in Hoyer’s solution ; 1 female , 3 males , North Carolina , Mecklenburg Co. Charlotte , VII-21-23-1991 , J.F. Cornell , lot 91 09754, in Hoyer’s solution. The above material from the USNM is labeled N. appalachiensis Plakidas and Ferro . Etymology. The specific name appalachiensis , Latin , “from the Appalachians”, in reference to the collection site of the type series from Tennessee , located along the western flank of the Appalachian Mountains.