Pseudococcidae (Hemiptera: Coccomorpha) in Uruguay: morphological identification and molecular characterization, with descriptions of two new species Author Pacheco Da Silva, Vitor C. Unidad de Entomología, Departamento de Protección Vegetal, Facultad de Agronomía, Universidad de la República, Montevideo, Uruguay Author Kaydan, Mehmet Bora Imamoglu Vocational School, Çukurova University, 01330, Adana, Turkey & Çukurova University, Biotechnology Application Research Centre, Balcalı, Adana, Turkey Author Basso, Cesar Unidad de Entomología, Departamento de Protección Vegetal, Facultad de Agronomía, Universidad de la República, Montevideo, Uruguay & cbasso. bis @ gmail. com; https: // orcid. org / 0000 - 0003 - 0427 - 3631 text Zootaxa 2020 2020-12-11 4894 4 501 520 journal article 9088 10.11646/zootaxa.4894.4.1 4f029708-021a-4c44-8e87-bb9ed9846cf6 1175-5326 4316657 EEC841C8-6550-4B44-B688-C020DED10671 Chorizococcus jeanfrancoisi Pacheco da Silva & Kaydan , sp. n. ( Fig. 3 ) Material studied. Holoype, adult female: URUGUAY : left label: Uruguay , 185 / Melilla / Bidens pilosa / Asteraceae / 13.iii.2019 / 34°44’48.9”S 56°17’22.1”W / coll. VCPS .; right label: Holotype / Chorizococcus jeanfrancoisi sp. n. / Pacheco da Silva & Kaydan. ECFA No. 185. Non-type material. Same data as holotype , 1 adult ♀ (in poor condition, not used for description or illustration) . GenBank accession number: MW 246246 for COI and MW 251836 for 28S. Appearance in life. Unknown. Species diagnosis. Chorizococcus jeanfrancoisi Pacheco da Silva & Kaydan sp. n. is characterized by the following combination of features: (i) antenna with 7 or 8 segments; (ii) circulus absent; (iii) 4 pairs of cerarii present on posteriormost abdominal segments; and (iv) translucent pores restricted to hind tibiae. TABLE 1. Identification of mealybugs ( Hemiptera : Pseudococcidae ) in Uruguay.
Mealybug species Host plant
Chorizococcus jeanfrancoisi Pacheco da Silva & Kaydan sp. n. Bidens pilosa L.
Chorizococcus rostellum (Lobdell) “ Poaceae ”
Dysmicoccus bonariensis Granara de Willink Gamochaeta coarctata (Willd.) Kerguélen
Ferrisia cristinae Kaydan & Gullan Citrus x limonia Osbeck
Ferrisia meridionalis Williams Cydonia oblonga Mill.
Phenacoccus madeirensis Green Aeonium sp.; Bulbine frutescens (L.) Willd.; Cestrum nocturnum L.; Chrysanthemum sp.; Cuphea hyssopifolia Kunth ; Fragaria x
ananassa (Weston) Duchesne ex Rozier; Gynura aurantiaca (Blume) DC ; Lavandula dentata L.; Pelargonium graveolens L’Hér ; Pelargonium hortorum L.H. Bailey ; Salvia farinacea Benth.
Phenacoccus montevidensis Pacheco da Silva & Kaydan sp. n. Helminthotheca echioides (L.) Holub
Phenacoccus peruvianus Granara de Willink Aucuba japonica Thunb. ; Bougainvillea sp.; Hypoestes phyllostachya Baker
Phenacoccus solenopsis Tinsley Capsicum annuum L.
Planococcus citri (Risso) Aeonium sp.; Cannabis sativa L.; Capsicum sp.; Citrus x limonia; Ficus lyrata Warb.; Hedera sp.; Hoya carnosa (L.) R.Br.; Ipomoea batatas (L.) Lam.; Kalanchoe coccinea var. Blossfeldiana (Poelln.) Boiteau; Nerium oleander L.; Solanum lycopersicum L.; Coleus scutellarioides (L.) Benth.
Planococcus ficus (Signoret) Ficus carica L.; Vitis vinifera L.
Pseudococcus cryptus Hempel Citrus limon (L.) Osbeck; Citrus × limonia
Pseudococcus longispinus (Targioni Tozzetti) Anoda sp.; Goeppertia zebrina (Sims) Nees ; Citrus sp.; Clivia miniata (Lindl.) Verschaff. ; Codiaeum variegatum (L.) Rumph. ex A.Juss.; Epipremnum aureum (Linden & André) G.S. Bunting ; Nerium oleander ; Platanus occidentalis L.
Pseudococcus pabulum Granara de Willink Erigeron bonariensis L.; Sonchus oleraceus L.; Trifolium pratense L.
Pseudococcus scatoterrae Granara de Willink Cydonia oblonga ; Malus domestica (Suckow) Borkh. ; Pyrus communis L.
Pseudococcus sociabilis Hambleton Citrus limon
Pseudococcus viburni (Signoret) Cydonia oblonga ; Bidens pilosa ; Citrus sp.; Cucurbita maxima Duchesne ; Malus domestica ; Mammillaria sp.; Nicotiana tabacum L.; Solanum lycopersicum . Solanum melongena L.; Sonchus oleraceus
Saccharicoccus sacchari Cockerell Saccharum officinarum L.
Spilococcus mamillariae (Bouché) Mammillaria sp.
Description. Adult female (based on holotype ). Body oval, 1.32 mm long, 0.66 mm wide. Eyes situated on margins, each 37.5 wide.Antenna with 7 or 8 segments, 280–290 long, with 5 fleshy setae, 3 on distal two segments and 2 on the basal segment, each seta 20.0–27.5 long; apical segment 77.5–82.5 long, 32.5–35.0 wide, with apical seta about 25.0 long. Clypeolabral shield 145 long, 142 wide. Labium 3-segmented, 125 long, 95 wide. Anterior spiracles each 48.5–52.5 long, 25.0–25.5 wide across atrium; posterior spiracles each about 55.0 long, 25.0–27.5 wide across atrium. Circulus absent. Legs well-developed; posterior leg segment lengths: coxa 245–250, trochanter + femur 390–400, tibia + tarsus about 400, claw about 30. Ratio of length of tibia + tarsus to trochanter + femur, 1.02–1.0:1; ratio of length of tibia to tarsus, 1.57–1.60:1; ratio of length of trochanter + femur to greatest width of femur, 2.88–2.96:1; tibia with 4–6 translucent pores. Tarsal digitules capitate, each 25.0–27.5 long. Claw digitules capitate, each about 20 long. Both pairs of ostioles present; anterior ostioles each with a total of 17 trilocular pores and 4 setae for both lips. Posterior ostioles each with a total of 18–21 trilocular pores and 0 setae for both lips. Anal ring about 87.5 wide, with 6 setae, each seta 107–125 long. Dorsum. Derm membranous, with 4 pairs of cerarii on posteriormost abdominal segments, each cerarius with 2 cerarian setae and a few auxiliary setae. Each anal lobe cerarius set on membranous cuticle and containing 2 enlarged setae each 20.0 long, and 15–16 trilocular pores. Dorsal setae flagellate, each 30.0–32.5 long, scattered throughout dorsum. Trilocular pores, each 3.00– 3.75 in diameter, and minute discoidal pores, each about 2.5 in diameter, scattered. Dorsal oral rim tubular ducts absent. Multilocular disc pores absent. Venter. Setae flagellate, each 12.5–55.0 long, longest setae located medially on head. Apical setae of anal lobes broken. Circulus absent. Multilocular disc pores each 7.0– 7.5 in diameter, present on abdomen, number on each abdominal segment as follows: I–II each with 0, III 18, IV 21, V 26, VI 30, VII 22, VIII + IX 2. Trilocular pores and minute discoidal pores scattered. Trilocular pores, each 3.00– 3.75 in diameter, scattered throughout venter. Oral collar tubular ducts of 2 sizes: (i) larger ducts, each 7.50–8.00 long, about 3.75 wide, present as segmental clusters on body margin and singly throughout, as follows: segments I–III each with 0, IV 5, V 43, VI 33, VII 31, VIII + IX 13; and (ii) smaller ducts, each 7.5–8.0 long, about 2.5 wide, present throughout, but in bands across abdominal segments, as follows: segments I–II each with 0, III 11, IV 17, V 6, VI 10, VII 12, VIII + IX 2. Comments. The new species most closely resembles Chorizococcus wilkeyi McKenzie and Chorizococcus variabilis McKenzie in having: (i) circulus absent, (ii) dorsum of abdomen without multilocular disc pores, and (iii) cerarii on at least the posteriormost 3 abdominal segments; however, C. jeanfrancoisi differs from both the other species in lacking oral rim tubular ducts on the dorsum. Etymology. The species epithet jeanfrancoisi was chosen in honor of the Coccomorpha taxonomist Jean-François Germain, from ANSES (Agence Nationale de Sécurité Sanitaire), France . Host plant. Bidens pilosa L. Distribution. Uruguay ( Montevideo , Melilla ). Molecular characterization. A BLAST hit with a sequence similarity of 98.67% to a 28S sequence assigned to Paracoccus galzerae Pacheco da Silva & Kaydan (KY565044.1) was obtained. This high similarity can be explained by the fact that Paracoccus is a non-monophyletic genus ( Hardy et al. 2008 ) that must be further studied. For COI, a BLAST hit of 94.55% of similarity was obtained with Atrococcus paludinus (Green) (KY372816.1). Comments. This new species is described based on one specimen, in excellent condition. The morphological characters were sufficiently different from other known species to consider this specimen to represent an undescribed species.