Studies on the free-living protozoan fauna of estuaries in the coastal zone of Tamil Nadu, India Author Bindu, L. text Records of the Zoological Survey of India 2021 121 1 61 75 journal article 10.26515/rzsi/v121/i1/2021/141506 2581-8686 13186453 32. Difflugia gramen Penard, 1902 Material examined : Mi-123, 2 ex. , Marakkanam estuary, 05.vii.2012 ; Mi-138, 4 ex. , Vellar estuary, 08.i.2013 ; Mi- 224, 3 ex. , Kaduviyar estuary, 06.xii.2013 ; Mi-249, 4 ex. , Vellar estuary, 20.vii.2014 ; Mi-269, 4 ex. , Arasalar estuary, 13.x.2015 . Diagnosis : Test ovoid, brown in colour, not compressed, composed of mineral particles of various sizes, surface of test more or less rough in appearance; aperture with three lobes having small ridges, often surrounded with a row of small granules; aperture sometimes with collar; diameter of test 61-125 µm. Distribution in India : Andhra Pradesh , Tamil Nadu (present record), West Bengal .