A review of insect parasitoids associated with Lobesiabotrana (Denis & Schiffermueller, 1775) in Italy. 1. DipteraTachinidae and HymenopteraBraconidae (Lepidoptera, Tortricidae) Author Scaramozzino, Pier Luigi Author Loni, Augusto Author Lucchi, Andrea text ZooKeys 2017 647 67 100 http://dx.doi.org/10.3897/zookeys.647.11098 journal article http://dx.doi.org/10.3897/zookeys.647.11098 1313-2970-647-67 80483F136B92468AB4CC5AD347ACD66F 80483F136B92468AB4CC5AD347ACD66F Phytomyptera nigrina (Meigen, 1824) (Pn) Fig. 2 Phytomyptera nigrina Laccone 1978 , Nuzzaci and Triggiani 1982 , Luciano et al. 1988 , Marchesini and Dalla Monta 1992 , 1994 , 1998 , Coscolla 1997 , Colombera et al. 2001 , Baur 2005 , Marchesini et al. 2006 , Bagnoli and Lucchi 2006 , Martinez et al. 2006 , Cerretti and Tschorsnig 2010 , Scaramozzino et al. (in press) . Phytomyptera unicolor Rond.: Del Guercio 1899 Phytomyptera nitidiventris Rond.: Silvestri 1912 , Catoni 1914 , Leonardi 1925 , Boselli 1928 , Stellwaag 1928 , Thompson 1946 Phytomyptera nitidiventris var. unicolor Rond.: Leonardi 1925 Phytomyptera spp. Moleas 1995 , Coscolla 1997 Italian distribution of reared parasitoids. Apulia: Nuzzaci and Triggiani 1982 , Laccone 1978 Campania: Silvestri 1912 (Portici, Nola) Piedmont: Colombera et al. 2001 , Baur 2005 Sardinia: Luciano et al. 1988 Tuscany: Del Guercio 1899 , Bagnoli and Lucchi 2006 , Scaramozzino et al. (in press) Umbria: Silvestri 1912 (Bevagna) Veneto: Marchesini and Dalla Monta 1992 , 1994 , 1998 , Marchesini et al. 2006 Emilia-Romagna: Baur 2005 (Bologna, leg. Campadelli) Distribution. North Central and South Europe, Russia North West, Ukraine (Fauna Europaea) Host range. Larval endophagous koinobiont parasitoid, Phytomyptera nigrina (see Tab. 3) recurs very often in all researches conducted in Italy on parasitoids of Lobesia botrana . Table 3. Phytomyptera nigrina : percentages of parasitism on the European Grapevine Moth reported in Italy by different authors.
Author/s, publication year Italian Region Host plant Year 1 st generation (antophagous) 2 nd generation (carpophagous) 3 rd generation (carpophagous)
Colombera et al. 2001
Colombera et al. 2001
Laccone 1978
Marchesini et al. 1994
Marchesini et al. 1994
Marchesini et al. 1994
Marchesini et al. 1994
Marchesini et al. 1994
Marchesini et al. 1994
Marchesini et al. 1994 Marchesini et al. 2006
Marchesini et al. 2006
Marchesini et al. 2006
Nuzzacci and Triggiani 1982 Daphne gnidium
Luciano et al. 1988 Daphne gnidium
This insect is associated to 29 species of Lepidoptera : Pterophoridae , Pyralidae , Sesiidae , Yponomeutidae and various genera and species of Tortricidae , included Eupoecilia ambiguella . Among the Tachinidae living on the vine moths, Pn shows the lowest number of hosts. For more details, see Martinez et al. (2006) and with regard to the hosts reported in Italy see Cerretti and Tschorsnig (2010) . As known, Pn larva hatches from an egg placed on the integument of the victim and, once actively penetrated, consumes its internal organs and kills it ( Bagnoli and Lucchi 2006 ). The existence of the puparium inside the host cocoon tight to the skin of the larva is a distinctive character for the species (Fig. 2F). Though Pn plays an important role in the natural control of Lobesia botrana , especially reducing the summer population ( Bagnoli and Lucchi 2006 , Thiery et al. 2006 ), it was not considered suitable for the control of Paralobesia viteana in the US, because of its relatively low host specificity, the low rate of parasitism reported in nature, and, referring in general to Tachinidae , due to previous experiences of unsuccessful releases ( Martinez et al. 2006 ). Figure 2. Phytomyptera nigrina (Meigen, 1824), female. A habitus, lateral view B habitus, dorsal view C head and anterior part of thorax, dorsal view D head, lateral view E wing F opened puparium tight to the skin of the EGVM dead larva.
Ecological role. Its importance as parasitoid depends on the host generation; indeed, various authors found that the parasitism rates are more generally related to the EGVM antophagous generation on grapevine: in this case they can overcome 25% of parasitism rate, both on grapevine in Apulia ( Laccone 1978 ) and on Daphne gnidium in Sardinia ( Luciano et al. 1988 ) (see table 2). In Tuscany, Phytomyptera nigrina (Pn) was mostly found in the vineyards of the medium and lower Arno valley, especially on larvae of the anthophagous generation ( Bagnoli and Lucchi 2006 ). In the natural reserve of San Rossore (Pisa), during several years of investigation carried out on Daphne gnidium , a single specimen of Pn was obtained from EGVM larvae of the second generation, collected in late July 2014 ( Scaramozzino et al. in press ), in contrast to observations carried out on the same host plant by other authors (see Table 3), whereas Actia pilipennis was more frequent in our case. In Piedmont, Pn reached on the first generation of EGVM and EGBM, in two successive years, significant parasitization rates (17.3 and 6.5%), but it was virtually absent (only two individuals obtained) in the second overwintering generation ( Colombera et al. 2001 ). Silvestri (1912) collected Pn from June to mid-October, Nuzzaci and Triggiani (1982) cited it as the more frequent parasitoid on Daphne gnidium in summer, with parasitism rates close to 30%. Laccone (1978) obtained Pn also in the second generation, with significant parasitization rates (from 11.4 to 14.7%). In Veneto, parasitization levels detected for this species were very low in the first generation (0.36 and 0.64%; Marchesini and Dalla Monta 1994 ), slightly higher, but with a significant 14.6%, in the second generation ( Marchesini et al. 2006 ). In France, Thiery et al. (2006) found Pn on the first generation of EGVM; they reported parasitization rates ranging from 5.2 to 41.2%. Pn has not been detected for the moment on EGVM overwintering generation, apart from what has been reported in the work of Colombera et al. (2001) .