Nitella sonderi A. Braun (Charales, Charophyceae) - a new record for South America, and first record from outside Australia Author Romanov, Roman E. Komarov Botanical Institute of the Russian Academy of Sciences, Prof. Popova str., 2, 197376 St. Petersburg (Russia) and Institute for Water and Environmental Problems, Siberian Branch of the Russian Academy of Sciences, Molodezhnaya str., 1, Barnaul, 656038 (Russia) romanov _ r _ e @ ngs. ru (corresponding author) e@ngs.ru Author Vidal-Russell, Romina INIBIOMA, CONICET - Universidad Nacional del Comahue, Bariloche, Río Negro (Argentina) vidalrussell @ comahue-conicet. gob. ar Author Nikulin, Vyacheslav Yu. Author Gontcharov, Andrey A. Federal Scientific Center of the East Asia Terrestrial Biodiversity, Far Eastern Branch of the Russian Academy of Sciences, 100 - letiya Vladivostoka Ave, 159, 690022, Vladivostok (Russia) nikulinvyacheslav @ gmail. com gontcharov @ biosoil. ru nikulinvyacheslav@gmail.com Author Quiroga, Maria Paula INIBIOMA, CONICET - Universidad Nacional del Comahue, Bariloche, Río Negro (Argentina) paulaquiroga @ comahue-conicet. gob. ar text Cryptogamie, Algologie 2022 2022-11-28 20 13 201 213 http://dx.doi.org/10.5252/cryptogamie-algologie2022v43a13 journal article 245031 10.5252/cryptogamie-algologie2022v43a13 19e22b3e-3f3a-4b64-ad33-d50ea6653556 1776-0992 7829496 Nitella sonderi A.Braun SPECIMENS EXAMINED. — Argentina . Neuquén Province , Alicurá Reservoir, 40°35’19”S , 70°52’34”W , 690 m a.s.l., 07.IV.2019 , P. Quiroga & R. Vidal-Russell (LE[A0000321, A0000322], in spirit) ( Figs 1-3 ). Australia . Queensland , 3.5 mi [ 5.6 km ] S of Stanthorpe post office, W of road, SW shore of metal (gravel) quarry, in clear water, 20.XI.1960 , R .D. Wood 60-11-20-22 ( LE [ A 0001490], GenBank accession: rbc L [ OM 311638 ]); 50.9 mi [ 81.9 km ] S of Miriam Vale on road to Gin Gin, abundant in c . eight inches of water, mud bottom, creek in deep valley (Black Creek?), 30.XI.1960 , R .D. Wood 60-11-30-10 ( LE [ A 0001491], GenBank accessions: rbc L [ OM 311639 ], ITS[ OM 338646 ]); 3.5 mi [ 6.5 km ] S of Stanthorpe, post office, W of road, selected slender specimens common along S shore of metal (gravel) quarry, 20.XI.1960 , R .D. Wood 60- 11-20-20 ( LE [ A 0001488]); Victoria , Benalla, N arm of Broken River, c . 300 yd. [ c . 274 m ] N of bridge (behind swimming pool), N shore of river, common at water’s edge, in c . 3 inches of fairly clear water, sandy gray mud bottom, 26.III.1963 , R .D. Wood 61- 3-26-2 ( LE [ A 0001489]). PHENOLOGY. — Male plants of N. sonderi were observed in the Patagonian locality during summer and autumn. A single plant producing antheridia has been successfully growing in an indoor aquarium since March 2020 . DESCRIPTION OF PATAGONIAN PLANTS Plants dioecious (only male plants were found), green, not encrusted with lime, unbranched, longer than 13 cm , with isolated homeoclemous whorls, neither condensed nor clumped at the apex ( Fig. 1 ), with obvious and wide, somewhat diffluent mucilage envelope of upper part of plants covering fertile whorls and internodes with a layer ( Fig. 2D, F, G ), approximately as thick as the diameter of the internodes and uniting whole branchlets within common envelope, sometimes resulting in lax apical heads ( Figs 1 ; 2C, D ). Stem diameter is (196-)337-509(-540) µm, increasing towards the basal part. Romanov R. E. et al . FIG. 1. – Apical parts of Nitella sonderi A.Braun from Argentina with sterile and fertile whorls (LE). Note: arrowhead , sterile whorls; double arrowhead , fertile whorls of male plants. Photo taken by R. E. Romanov. Scale bar: 1 cm. The transversely elliptical cells extending from the base of the branchlet and forming a ring surrounding it are easily recognisable at appropriate magnification ( Fig. 3A, B ). The pair of these cells is commonly present below the branchlet base, one of the cells as an exception can be missing. These cells contain chloroplasts, not starch, and are not discoloured, 42-109 µm in length, 71-164 µm in width. Their growth seems to be able to culminate in nodal bulbil formation, recognisable as overgrowth of stem nodes inside and outside of the whorl before destruction of branchlets. The sterile and fertile branchlets are macrodactylous, with similar principal arrangement and quite uniform appearance within the same whorl. However, fertile ones have shorter penultimate rays, resulting in a tassel-like appearance at the ends of the central rays because of aggregated dactyls (cf. Figs 1 ; 2 A-D). The sterile branchlets are 2-2.8-times shorter than the internodes; the length of internodes and the proportion between internode and branchlet length decrease towards the apex. The whorls of sterile branchlets are 32-44 mm in span, more lax and diffuse, spreading more in contrast to fertile ones, which are shorter and compact at their ends, with a diameter of (10-) 16-29 mm . The length of sterile branchlets is (10.9-)12.5-21.5(-23)mm,withprimaryrays(5-)7.7-9.5(-10)mm long, i.e., half of the total branchlet length or slightly shorter.The lateral secondary rays of sterile branchlets are 3.5-6(-6.5) mm in length, whereas the central secondary ones are approximately 4.5-5 mm . FIG. 2. – Male plants of Nitella sonderi A.Braun from Argentina (LE): A , whorl of sterile branchlets; B , sterile branchlet; C , apex with whorls of fertile branchlets forming lax head; D , lax apical head consisting of fertile branchlets, embedded in mucilage 3-furcate “prolification” looking as a fertile branchlet without a central secondary ray from the lateral tertiary ray at the furcation of the central secondary ray ( double arrowhead ); E , last furcations of fertile branchlets with long straight dactyls; F , fertile branchlet with antheridia obviously unequal each other; G , mucilage cover of fertile branchlet ( double arrowhead ), mucilage cover of branchlet primary ray; H , antheridia at last furcations of branchlet surrounded with short dactyls, arcuate at their basal parts. Note: A -C , arrowheads represent central secondary ray; D , F , G , arrowheads represent surface of mucilage cover. Photos taken by R. Vidal-Russell. Scale bars: A, E, F, 2 mm; B-D, 5 mm; G, H, 1 mm. FIG. 3. – Male plants of Nitella sonderi A.Braun from Argentina (LE): A , B , cells extending from the node at the base of the lowest branchlet cell at different focus ( arrowheads at A ); C -E , shortly narrowing ends of bicellulate dactyls with discoloured tiny confluent end cells having thickened cell walls at the tip; F , end of dactyl after loss of end cell; G , H , triangular shields of antheridia. All photos taken by R. E. Romanov, but H by R. Vidal-Russell. Scale bars: 100 μm. The fertile branchlets form at apical parts of the plants ( Fig. 1 ). The length of fertile branchlets is 9-15 mm , with primary rays having a length of 5-8.5 mm , i.e., mostly somewhat exceeding half of the total branchlet length. The primary ray at the basal part is 155-382 µm in diameter, and in the apical part, in fertile whorls, it is 110-235 µm in diameter. The lateral and central secondary rays of fertile branchlets are shorter in absolute values (approximately two times less in case of the latter ones) in comparison with sterile branchlets. Each branchlet is 2-3-times forked, with a central secondary ray, surrounded by 6-8 more or less equal or somewhat shorter lateral secondary rays (sometimes c . 0.4-0.7 the length of the central secondary ray), mostly not differing in width from the central one, but sometimes obviously more robust ( Fig. 2A, C, F, G ). As an exception, the branchlet can produce 3-furcate “prolification”, looking like a fertile branchlet without a central secondary ray. It forms from the lateral tertiary ray at the central secondary ray furcation ( Fig. 2D ). The lateral secondary rays are 1- or 1- and 2-times forked; the latter is less frequent at sterile branchlets in contrast with the common pattern of fertile ones. The lateral secondary rays are longer than the dactyls in sterile whorls and longer, nearly equal to mostly slightly or obviously shorter than the dactyls in fertile whorls (to approximately 0.8 of dactyl length). The dactyls of 1- and 2-times forked lateral secondary rays in sterile whorls are 1.8-2-times shorter than those in tertiary rays. The central tertiary ray is neither formed at the furcation of lateral rays nor at the central secondary ray; the tertiary rays are 1-3 (or more?) in furcation and, as a rule, shorter than dactyls. The central secondary rays are 2-times furcated. The dactyls are 2-4(-5) in sterile branchlets, and 5-6 in fertile branchlets, strictly bicellulate, 0.8-2.8(-3) mm in length in sterile branchlets, 0.8-1.3 mm long in fertile branchlets, at second and third furcations, and at the first furcation of sterile branchlets, straight at sterile and fertile nodes in case of dactyls significantly longer of the antheridium, usually more or less arcuate in their basal parts at nodes with the antheridium in case of short dactyls, 1.2-2-5-times longer than the antheridium ( Fig. 2C , E-H). The penultimate cell is long cylindrical with a shortly narrowing end ( Fig. 3 C-E). The base of the end cell is confluent with the tip of penultimate cells. The end cell is small, conical, pointed, straight or somewhat curved through the whole length or only at the tip, mostly discoloured in living, pressed and fixed states. The dactyls appear single-celled at lower magnification because of tiny and mostly discoloured confluent end cells. The width of dactyls at their base (above the antheridium) is (112-)130-250 µm, at the apical part (below the shortly narrowing end) it is 123-187 µm. The end cells are 46-68 µm in length and 15-31 µm in width. The tip of the end cell has an obviously thickened cell wall ( Fig. 2 C-D). The end cells can be lost over time ( Fig. 3F ). The antheridia are solitary, strictly terminal at the second and third furcations of the branchlet, never at the first one, sessile, round with a slightly attenuated base, octoscutate (i.e., with triangular shields; Fig. 3G, H ), frequently looking robust in contrast with the length and width of dactyls surrounding them ( Fig. 2 E-H), (370-)399-655(-762)µm in diameter; their diameter can be different within the same branchlet ( Fig. 2F ).