Morphological and molecular characterization of twenty-five new Diploneis species (Bacillariophyta) from Lake Tanganyika and its surrounding areas Author Jovanovska, Elena 0000-0002-3413-3683 Department of Paleoanthropology, Senckenberg Research Institute and Natural History Museum Frankfurt, Frankfurt, Germany & jovanovska. eci @ gmail. com; https: // orcid. org / 0000 - 0002 - 3413 - 3683 jovanovska.eci@gmail.com Author Wilson, Mallory C. 0000-0002-2852-125X Department of Paleoanthropology, Senckenberg Research Institute and Natural History Museum Frankfurt, Frankfurt, Germany & Indiana State University, Indiana State University, Terre Haute, IN, USA & mwilson 108 @ sycamores. indstate. edu; https: // orcid. org / 0000 - 0002 - 2852 - 125 X mwilson108@sycamores.indstate.edu Author Hamilton, Paul B. 0000-0001-6938-6341 Phycology Section, Research and Collections Division, Canadian Museum of Nature, Ottawa, Canada & phamilton @ nature. ca; https: // orcid. org / 0000 - 0001 - 6938 - 6341 phamilton@nature.ca Author Stone, Jeffery 0000-0002-1313-0643 Department of Paleoanthropology, Senckenberg Research Institute and Natural History Museum Frankfurt, Frankfurt, Germany & Indiana State University, Indiana State University, Terre Haute, IN, USA & Department of Paleoanthropology, Senckenberg Research Institute and Natural History Museum Frankfurt, Frankfurt, Germany & jeffery. stone @ indstate. edu; https: // orcid. org / 0000 - 0002 - 1313 - 0643 * Corresponding author & Department of Paleoanthropology, Senckenberg Research Institute and Natural History Museum Frankfurt, Frankfurt, Germany jeffery.stone@indstate.edu text Phytotaxa 2023 2023-04-21 593 1 1 102 http://dx.doi.org/10.11646/phytotaxa.593.1.1 journal article 10.11646/phytotaxa.593.1.1 ef558f00-24a4-4671-bf56-df3c1d61ecd1 1179-3163 7875089 Diploneis fossa sp. nov. (LM Figs 178–185 , SEM Figs 186–192 ) Valves are weakly asymmetric, elliptical to linear–elliptical with parallel margins and bluntly round apices ( Figs 178–188 ). The length of the valve is 47.5–64 μm and the width of the valve is 23–27 μm. The axial area is narrow, lanceolate, widening at the center to form a longitudinally elongate and weakly asymmetric central area ( Figs 178 , 187, 190 ), 4–5 μm wide. Externally, the canal is lanceolate and slightly expanded in the middle of the valve with three rows of cribrate (<15 poroids) areolae narrowing into one at the valve apices ( Fig. 189 ). Externally, the raphe is filiform, curved with simple proximal ends bent to the same side of the valve; the proximal fissures are positioned in expanded teardrop depressions ( Figs 187, 190 ). The distal raphe ends are unilaterally bent to the same side and terminate at the valve face mantle junction ( Figs 188, 189, 192 ). The striae are parallel at mid-valve becoming radiate towards the valve apices, 8–9 in 10 μm. Striae are uniseriate throughout ( Figs 189, 191 ). The striae are composed of large round to rectangular areolae covered externally with fine pored cribra (>45 poroids), 8–10 in 10 μm. Externally, each areola opens into deep pits. The inter-areolar thickenings have fin-like crest silica ridges serrated with ca. 9–12 fine notched edges ( Fig. 189 ). The fin-like ridges along the canal are slightly bent into semi-circular shape, positioned towards the striae whereas those of the striae are only slightly bent positioned towards the canal, changing opposite direction only at valve mantle ( Figs 187, 192 ). Type:— REPUBLIC OF ZAMBIA , Lake Tanganyika , Isanga Bay , at 768 m elevation; rock scrape, 20 m water depth, collected SCUBA diving, 8°39’10.7” S 31°11’34.8” E , A. Jordan , 8 th October 2021 ( holotype designated here, circled specimen BM-108982! = Fig. 184 , isotypes ANSP-GC17211 !, CANA-129334!). Type material CANA-129329. Registration: http://phycobank.org/103708 Pictures of the isolated specimen:— LM micrograph on 1000× magnification ( Fig. S2c ). Sequence data:— Plastid gene rbc L sequence (GenBank accession: OQ 660301). Etymology:— The specific epithet ‘ fossa ’ refers to the ditch-like formation of the fin-like crest ridges. Ecology and distribution:— This species has only been observed on the Zambian and Tanzanian coasts of Lake Tanganyika. In the alkaline, moderately mineral-rich and highly transparent waters, it inhabits sandy and muddy substrates (sometimes with mollusk shells) and submerged rocks between 5 and 33 m water depth. The largest population sizes of this species have been found in the southern parts of the lake in Isanga Bay and Mutondwe Island. Smaller population sizes were found in Chituta Bay, Kalambo Falls Lodge, Ndole Bay and in the central parts of the lake in Kalya Bay and Mahale National Park ( Fig. 1c, e, f ). In the type material, it mostly coexists with D. major sp. nov. while at all other sites it occurs with D. kilhamiana sp. nov. , D. cocquytiana sp. nov. , D. tumida sp. nov. , D. cristata sp. nov. , D. salzburgeri sp. nov. , D. serrulata sp. nov. , and D. duplex sp. nov. Main differential characters:— Valve shape, striae pattern, striae density, external fin-like ornamentations across the valve, fins scattered on the longitudinal canals, areolae formation over the longitudinal canal (thickened virgae), and poroids>45 per areola. Similar species:— Diploneis elongata sp. nov. and Diploneis raetica Lange-Bertalot & Fuhrmann (2020: 119) .