Ophiostomatoid species associated with pine trees (Pinus spp.) infested by Cryphalus piceae from eastern China, including five new species Author Chang, Runlei https://orcid.org/0000-0001-6613-9062 College of Life Sciences, Shandong Normal University, Jinan 250014, China Author Zhang, Xiuyu College of Life Sciences, Shandong Normal University, Jinan 250014, China Author Si, Hongli https://orcid.org/0000-0003-0585-3478 College of Life Sciences, Shandong Normal University, Jinan 250014, China Author Zhao, Guoyan College of Life Sciences, Shandong Normal University, Jinan 250014, China Author Yuan, Xiaowen Kunyushan Forest Farm, Yantai 264112, China Author Liu, Tengteng https://orcid.org/0000-0002-0182-2562 College of Life Sciences, Shandong Normal University, Jinan 250014, China Author Bose, Tanay Forestry and Agricultural Biotechnology Institute (FABI), Department of Biochemistry, Genetics & Microbiology, University of Pretoria, Pretoria 0002, South Africa Author Dai, Meixue College of Life Sciences, Shandong Normal University, Jinan 250014, China daimeixue@sdnu.edu.cn text MycoKeys 2021 2021-10-13 83 181 208 http://dx.doi.org/10.3897/mycokeys.83.70925 journal article http://dx.doi.org/10.3897/mycokeys.83.70925 1314-4049-83-181 44A7323B8C5254CFAF89C7FCB718EDC2 3. Graphilbum translucens R.L. Chang & X.Y. Zhang sp. nov. Fig. 11 Holotype. China. Shandong province: Zhujiajuan village, Huancui District, Weihai City, from the gallery of Cryphalus piceae on Pinus thunbergii , 10 Oct. 2019, R. L. Chang (HMAS 249925-holotype; SNM144 = CGMCC 3.20263 - ex-holotype culture). Additional cultures checked. China. Shandong province: Laojiangou village, Laoshan District, Qingdao City, from the gallery of Cryphalus piceae on Pinus densiflora , 2, Aug. 2020, R. L. Chang (SNM104). Etymology. The name refers to the translucent appearance of the colony on MEA. Diagnosis. Graphilbum translucens can be distinguished from other closely related species, Gr. puerense and Gr. acuminatum , by the shorter hyalorhinocladiella-like conidiophores, smaller conidia and no pesotum-like asexual state. Description. Sexual morph is unknown. Asexual state hyalorhinocladiella-like: the conidiophores directly arising from the vegetative hyphae, measuring (3.6-) 8.6-42.2 (-72.3) μm x (0.9-) 1.1-1.7 (-2.0) μm (Fig. 11b-e ); conidia hyaline, smooth, unicellular short oblong, with rounded ends or ellipsoidal to ovoid, measuring (2.1-) 2.4-3.5 (-4.1) x (0.8-) 1.3-2.0 (-2.7) μm (Fig. 11b-e ). Figure 11. Morphological characters of asexual structures of Graphilbum translucens sp. nov. a fourteen-day-old culture on MEA b-e conidiophores and conidia. - Scale bars: 10 μm . Culture characteristics. The colonies are light brown in color on MEA (Fig. 11a ). Mycelia are partially submerged in the agar. The optimal temperature for growth is 30 °C, reaching 74.0 mm diam in 5 days. Growth slower at 35°C, 24 mm diam in 5 days. No growth was observed at 5 °C. Distribution. Currently known from Qingdao City and Weihai City in Shandong Province, China. Note. Based on morphology coupled with single-gene (ITS, EF, BT, and CAL) phylogenies, Graphilbum translucens is phylogenetically close to Gr. puerense and Gr. acuminatum . In the ITS tree, Gr. translucens grouped with Gr. puerense (Fig. 3 ) and Gr. acuminatum whereas it formed distinct clades in the BT and EF trees (Figs 4 and 5 ). The hyalorhinocladiella-like asexual state was observed in Gr. translucens and Gr. puerense , but it is absent in Gr. acuminatum ( Chang et al. 2017 ; Jankowiak et al. 2020 ). The conidiophores of Gr. translucens are shorter than the Gr. puerense ( Chang et al. 2017 ). Conidia of Gr. translucens and Gr. puerense form hyalorhinocladiella-like asexual states that are similar in shape, yet the conidia size of Gr. translucens is smaller than Gr. puerense (2.1-4.1 x 0.8-2.7 vs. 3.5-12 x 1-3 μm , Fig. 11b-e ) ( Chang et al. 2017 ). Unlike Gr. puerense and Gr. acuminatum , a pesotum-like asexual state was not observed among the isolates of Gr. translucens recovered in this study.