Calcarisporium yuanyangense sp. nov. (Calcarisporiaceae, Hypocreales), a fungal pathogen isolated from Ophiocordyceps nutans Author Zhu, Juye 0000-0002-4184-5646 Yunnan Herbal Laboratory, College of Ecology and Environmental Sciences, Yunnan University, Kunming 650504, China School of Life Science, Yunnan University, Kunming, Yunnan 650504, China & 2214416799 @ qq. com; https: // orcid. org / 0000 - 0002 - 4184 - 5646 2214416799@qq.com Author Tang, Dexiang 0000-0002-7662-224X Yunnan Herbal Laboratory, College of Ecology and Environmental Sciences, Yunnan University, Kunming 650504, China School of Life Science, Yunnan University, Kunming, Yunnan 650504, China & 1347974204 @ qq. com; https: // orcid. org / 0000 - 0002 - 7662 - 224 X 1347974204@qq.com Author Liu, Zuoheng 0000-0003-4118-3694 Yunnan Herbal Laboratory, College of Ecology and Environmental Sciences, Yunnan University, Kunming 650504, China School of Life Science, Yunnan University, Kunming, Yunnan 650504, China & 2416338332 @ qq. com; https: // orcid. org / 0000 - 0003 - 4118 - 3694 2416338332@qq.com Author Luo, Lijun 0000-0002-1709-0781 Yunnan Herbal Laboratory, College of Ecology and Environmental Sciences, Yunnan University, Kunming 650504, China School of Life Science, Yunnan University, Kunming, Yunnan 650504, China & 1211918264 @ qq. com; https: // orcid. org / 0000 - 0002 - 1709 - 0781 1211918264@qq.com Author Yu, Hong 0000-0002-2149-5714 Yunnan Herbal Laboratory, College of Ecology and Environmental Sciences, Yunnan University, Kunming 650504, China School of Life Science, Yunnan University, Kunming, Yunnan 650504, China & hongyu @ ynu. edu. cn, herbfish @ 163. com; https: // orcid. org / 0000 - 0002 - 2149 - 5714 text Phytotaxa 2023 2023-09-06 612 3 272 282 http://dx.doi.org/10.11646/phytotaxa.612.3.3 journal article 266699 10.11646/phytotaxa.612.3.3 a8f51ab4-9038-4f4c-9941-efceaa10b65c 1179-3163 8323590 Calcarisporium yuanyangense H. Yu bis & J.Y. Zhu, sp. nov. Fig. 2 MycoBank: MB 848860 Etymology:— The epithet “ yuanyangense ” referred to collection sites for this species, Yuanyang County. Holotype :— Xinjie Town , Yuanyang County , Yunnan Province , China ( 23°04′42.98″N , 102°51′41.61″E , alt. 2053.70m ), on an O. nutans , September 25, 2022 , Hong Yu bis , YHH 2209025 , holotype; YFCC 22099256 , ex-type living culture. Description:—Sexual morph: Sexual morphs were not found. Asexual morph: Colonies effuse, woolly-felty texture, yellowish-white, mycelium immersed, consisting of branched, septate, smooth, hyaline hyphae, hyphae 1.81– 2.89 μm wide. colonies on PDA reached 20–22 mm in diameter after 10 days at 25 ℃. Conidiophores 12.49–119.42 × 1.49–3.01 μm, erect, hyaline, verticillately branched, with 1–5 verticils, each verticil with 1–5 conidiogenous cells. Conidiogenous cells 12.31–51.84 × 1.40–3.08 μm, hyaline, swollen at base, gradually tapering near the apex, holoblastic to polyblastic, sympodial, apically with a cluster of conidium-bearing denticles. conidiogenous scars 0.86–1.78μm. Conidia 6.33–9.68 × 1.87–2.63 μm, unicellular, hyaline, smooth-walled, thin-walled, ovoid to ellipsoid, acuminate. Know distribution:— Xinjie Town, Yuanyang County, Yunnan Province , China . Note:— Calcarisporium yuanyangense was isolated from O. nutans and was different from the host reported in previous studies. Calcarisporium yuanyangense was identified as a unique species based on both morphological characteristics and phylogenetic analysis; both demonstrate that it was distinct from other recognized species in the genus Calcarisporium . Phylogenetic analysis based on five genes showed strong support (BP = 100% and PP = 100%) with being an independent branch. Calcarisporium yuanyangense and C. arbuscula were similar in conidia shape, but the conidia of C. yuanyangense (6.33–9.68 × 1.87–2.63 μm) were larger and had a higher length/width ratio than those of C. arbuscula ( Table 2 ).